TSH beta Antibody (512908) [Unconjugated]

Western blot shows purified human pituitary TSH beta (10 ng/lane). PVDF Membrane was probed with 2 µg/mL of Human TSH beta Monoclonal Antibody (Catalog # MAB57941) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band was detected for TSH beta at approximately 25 kDa (as indicated). This experiment was conducted under non-reducing conditions and using Immunoblot Buffer Group 1.

TSH beta was detected in immersion fixed paraffin-embedded sections of human pituitary using Human Thyroid Stimulating Hormone Subunit Beta Monoclonal Antibody (Catalog # MAB57941) at 15 µg/mL overnight at 4 °C. Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (Catalog # CTS013). Tissue was stained using the Anti-Mouse HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS002) and counterstained with hematoxylin (blue). Specific staining was localized to cytoplasm of thyrotrope cells. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.

• Reactivity: Hu
• Applications: WB, IHC
• Clone: 512908
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Concentration: LYOPH

TSH beta Antibody (512908) [Unconjugated] Summary

• Immunogen: Chinese hamster ovary cell line CHO-derived recombinant human TSH beta
  Phe21-Val138
  Accession # NP_000540
• Specificity: Detects TSH beta in direct ELISAs and Western blots. In direct ELISAs, no cross-reactivity with recombinant rat TSH beta was observed.
• Source: N/A
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Mouse
• Gene: TSHB
• Purity Statement: Protein A or G purified from hybridoma culture supernatant

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry 8-25 ug/mL
  • Western Blot 2 ug/mL

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Preservative: No Preservative
• Concentration: LYOPH
• Reconstitution Instructions: Sterile PBS to a final concentration of 0.5 mg/mL.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for TSH beta Antibody (512908) [Unconjugated]

• thyroid stimulating hormone, beta
• thyroid-stimulating hormone subunit beta
• thyrotropin beta chain
• thyrotropin beta subunit
• Thyrotropin beta
• thyrotropin subunit beta
• TSH beta
• TSHB
• TSH-B
• TSH-BETA

Background

TSH beta is the 138 amino acid (aa), 15 kDa unique subunit of Thyroid Stimulating Hormone (TSH), which shares a common alpha subunit with other glycoprotein hormones lutropin (LH), follitropin (FSH) and chorionic gonadotropin (CG). Production of TSH by the anterior pituitary gland is stimulated by the hypothalamic peptide TRH, and inhibited by feedback from the thyroid hormones triiodothyronine (T3) and thyroxine (T4). Mature human TSH beta shares 89% and 90% aa sequence identity with mouse and rat TSH beta , respectively.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: TSHB
• Uniprot:
  • NP_000540()