TSH beta Antibody (442506) [Unconjugated]

• Reactivity: Rt
• Applications: WB
• Clone: 442506
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Concentration: LYOPH

TSH beta Antibody (442506) [Unconjugated] Summary

• Immunogen: E. coli-derived recombinant rat TSH beta
  Phe21-Gly138
  Accession # P04652
• Specificity: Detects rat TSH beta in direct ELISAs and Western blots. In direct ELISAs and Western blots, this antibody shows approximately 5% cross‑reactivity with recombinant human TSH beta .
• Source: N/A
• Isotype: IgG2b
• Clonality: Monoclonal
• Host: Mouse
• Gene: TSHB
• Purity Statement: Protein A or G purified from hybridoma culture supernatant

Applications/Dilutions

• Dilutions:
  • Western Blot 1 ug/mL

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS and NaCl with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Preservative: No Preservative
• Concentration: LYOPH
• Reconstitution Instructions: Reconstitute at 0.5 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for TSH beta Antibody (442506) [Unconjugated]

• thyroid stimulating hormone, beta
• thyroid-stimulating hormone subunit beta
• thyrotropin beta chain
• thyrotropin beta subunit
• Thyrotropin beta
• thyrotropin subunit beta
• TSH beta
• TSHB
• TSH-B
• TSH-BETA

Background

TSH beta is the 138 amino acid (aa), 15 kDa unique subunit of Thyroid Stimulating Hormone (TSH), which shares a common alpha subunit with other glycoprotein hormones lutropin (LH), follitropin (FSH) and chorionic gonadotropin (CG). Production of TSH by the anterior pituitary gland is stimulated by the hypothalamic peptide TRH, and inhibited by feedback from the thyroid hormones triiodothyronine (T3) and thyroxine (T4). Mature rat TSH beta shares 92% and 90% aa sequence identity with mouse and human TSH beta , respectively.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for TSH beta Antibody (MAB4507)(1)

Publication using MAB4507

• H Wu, W Zhang, Y Zhang, Z Kang, X Miao, X Na Novel insights into di?(2?ethylhexyl)phthalate activation: Implications for the hypothalamus?pituitary?thyroid axis Molecular Medicine Reports, 2021-03-02;23(4):. 2021-03-02 [PMID: 33649816] (Western Blot, Rat)

Bioinformatics

• Gene Symbol: TSHB
• Uniprot:
  • P04652()