TSC1 Antibody

Western Blot: TSC1 Antibody [NB100-80855] - TSC1 in C2C12 cell lysate with TSC1 antibody at (A) 2 and (B) 4 ug.

Immunohistochemistry-Paraffin: TSC1 Antibody [NB100-80855] - Staining of human skeletal muscle. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.

Immunohistochemistry-Paraffin: TSC1 Antibody [NB100-80855] - Staining of human heart. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.

Product Discontinued

TSC1 Antibody Summary

• Immunogen: A 15 amino acid synthetic peptide from the C-terminus of human TSC1. The immunogen is located within amino acids 850 - 900 of TSC1.
• Epitope: Near the center, aa 850-900
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: TSC1
• Purity: Peptide affinity purified

Applications/Dilutions

• Dilutions:
  • ELISA
  • Immunohistochemistry 5 ug/ml
  • Immunohistochemistry-Paraffin 5 ug/ml
  • Western Blot 4 ug/ml
• Control:

  TSC1 Overexpression Lysate

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS
• Preservative: 0.02% Sodium Azide
• Concentration: 1.0 mg/ml
• Purity: Peptide affinity purified

Alternate Names for TSC1 Antibody

• Hamartin
• KIAA0243MGC86987
• LAMhamartin
• TSC
• TSC1
• Tuberous sclerosis 1 protein
• tuberous sclerosis 1
• tumor suppressor

Background

Hamartin, or TSC1, is a suspected tumor suppressor implicated in the disease tuberous sclerosis 1. It is a negative regulator of cell division controlling the transition from G0/G1 to S phase, and it seems to act through the phosphatidylinositol 3 kinase/Akt pathway. TSC1 interacts with tuberin m(TSC2), which is thought to be a GAP (GTPase Activating Protein) for the Rap1 and Rab5 small G Proteins. The Hamartin/Tuberin complex has been shown to inhibit mTor. Hamartin has also been shown to interact with ERM (Ezrin-Radixin-Moesin) proteins and with F-actin, suggesting a role for TSC proteins in modulation of cell adhesion and morphology.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: TSC1
• Entrez:
  • 7248(Human)
  • 64930(Mouse)
• Uniprot:
  • Q92574()