Recombinant Human Trypsin 3/PRSS3 Protein

Product Discontinued

Recombinant Human Trypsin 3/PRSS3 Protein Summary

• Description: A denatured recombinant protein with a N-Terminal His-tag and corresponding to the amino acids 81-304 of Human Trypsin 3/PRSS3
  
  

  Source: E.coli

  Amino Acid Sequence: MGSSHHHHHH SSGLVPRGSH MGSIVGGYTC EENSLPYQVS LNSGSHFCGG SLISEQWVVS AAHCYKTRIQ VRLGEHNIKV LEGNEQFINA AKIIRHPKYN RDTLDNDIML IKLSSPAVIN ARVSTISLPT APPAAGTECL ISGWGNTLSF GADYPDELKC LDAPVLTQAE CKASYPGKIT NSMFCVGFLE GGKDSCQRDS GGPVVCNGQL QGVVSWGHGC AWKNRPGVYT KVYNYVDWIK DTIAANS

• Source: E. coli
• Protein/Peptide Type: Recombinant Protein
• Gene: PRSS3
• Purity: >90%, by SDS-PAGE

Applications/Dilutions

• Dilutions:
  • SDS-Page
• Application Notes: Denatured protein is most likely not the best option for functional studies. It is better suited for Western Blot (WB) or imaging assays.
• Theoretical MW: 26 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: 20 mM Tris-HCl buffer (pH 8.0), 0.4M UREA, 10% glycerol
• Preservative: No Preservative
• Concentration: 1 mg/ml
• Purity: >90%, by SDS-PAGE

Alternate Names for Recombinant Human Trypsin 3/PRSS3 Protein

• Brain trypsinogen
• EC 3.4.21
• EC 3.4.21.4
• Mesotrypsin
• mesotrypsinogen
• MTG
• pancreatic trypsinogen III
• protease, serine, 3 (mesotrypsin)
• protease, serine, 3
• protease, serine, 4 (trypsin 4, brain)
• PRSS3
• PRSS4
• Serine protease 3
• Serine protease 4
• T9
• TRY3MTG
• TRY4mesotrypsin
• Trypsin 3
• Trypsin 4
• Trypsin III
• Trypsin IV
• trypsin-3
• Trypsinogen 15
• trypsinogen 4
• trypsinogen 5
• trypsinogen IV

Background

PRSS3 is a trypsinogen, which is a member of the trypsin family of serine proteases. This enzyme is expressed in the brain and pancreas and is resistant to common trypsin inhibitors. It is active on peptide linkages involving the carboxyl group of lysine or arginine. This gene is localized to the locus of T cell receptor beta variable orphans on chromosome 9. Four transcript variants encoding different isoforms have been described for this gene. Recombinant human PRSS3 protein, fused to His-tag at N-terminus, was expressed in E.coli.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Peptides and proteins are guaranteed for 3 months from date of receipt.

Bioinformatics

• Gene Symbol: PRSS3