Trypsin 3/PRSS3 Antibody (420025) [Alexa Fluor™ Plus 594]

• Reactivity: Hu
• Clone: 420025
• Clonality: Monoclonal
• Host: Rat
• Conjugate: Alexa Fluor Plus 594

Trypsin 3/PRSS3 Antibody (420025) [Alexa Fluor™ Plus 594] Summary

• Specificity: Detects human Trypsin 3/PRSS3 in Western blots. In Western blots, no cross-reactivity with recombinant human (rh) Trypsin-1 or rhTrypsin-2 is observed.
• Isotype: IgG2a
• Clonality: Monoclonal
• Host: Rat

Packaging, Storage & Formulations

• Storage: Protect from light. Do not freeze. 12 months from date of receipt, 2 to 8 °C as supplied
• Buffer: Supplied 0.2 mg/mL in a saline solution containing BSA and Sodium Azide.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Trypsin 3/PRSS3 Antibody (420025) [Alexa Fluor™ Plus 594]

• Brain trypsinogen
• EC 3.4.21
• EC 3.4.21.4
• Mesotrypsin
• mesotrypsinogen
• MTG
• pancreatic trypsinogen III
• protease, serine, 3 (mesotrypsin)
• protease, serine, 3
• protease, serine, 4 (trypsin 4, brain)
• PRSS3
• PRSS4
• Serine protease 3
• Serine protease 4
• T9
• TRY3MTG
• TRY4mesotrypsin
• Trypsin 3
• Trypsin 4
• Trypsin III
• Trypsin IV
• trypsin-3
• Trypsinogen 15
• trypsinogen 4
• trypsinogen 5
• trypsinogen IV

Background

Human Trypsin 3, encoded by the PRSS3 gene, is also known as mesotrypsin (1). Constituting less than 10% of the total trypsin content in normal pancreatic juice, it is one of the three trypsin isoforms produced by the pancreas (2). Compared to trypsin 1 and 2, one intriguing feature of Trypsin 3 is its resistance to polypeptide trypsin inhibitors, such as the Kunitz-type soybean trypsin inhibitor or the Kazal-type pancreatic secretory trypsin inhibitor. As revealed by the crystal structure, this resistance is likely due to the presence of an arginine residue in place of the highly conserved Gly198 (3). Trypsin 3 is synthesized in the pancreas and secreted into the duodenum lumen, where it is activated by enterokinase. One proposed physiological function of Trypsin 3 is degradation of trypsin inhibitors, which facilitates the digestion of those foods rich in these proteins (4).

1. Nyaruhucha, C. N. M. et al. (1997) J. Biol. Chem. 272:10573.
2. Rinderknecht, H. et al. (1984) Gastroenterology 86:681.
3. Katona, G. et al. (2002) J. Mol. Biol. 315:1209.
4. Szmola, R. et al. (2003) J. Biol. Chem. 278:48580.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.