Trypsin 1/PRSS1 Antibody - BSA Free

Western Blot: Trypsin 1/PRSS1 Antibody [NBP2-44111] - Western Blot of Trypsin 1/PRSS1 1/PRSS1 antibody. Lane 1: Trypsin 1/PRSS1 (Bovine). Load: 50 ng per lane.Primary antibody: Trypsin 1/PRSS1 antibody at 1:1,000 overnight at 4C.Secondary antibody: Peroxidase rabbit secondary antibody at 1:40,000 for 30 min at RT.Block: Blocking Buffer for Fluorescent Western Blotting for 30 min at RT.Predicted/Observed size: 25 kDa for Trypsin 1/PRSS1. Other band(s): Low molecular weight bands could correspond to autocatalytic fragments.

• Reactivity: Bv
• Applications: WB, ELISA
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: BSA Free
• Concentration: LYOPH

Trypsin 1/PRSS1 Antibody - BSA Free Summary

• Description: Trypsin Antibody is an IgG fraction purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated above. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Rabbit Serum as well as purified and partially purified Trypsin (Bovine Pancreas)
  
  Store vial at 4C prior to restoration. For extended storage aliquot contents and freeze at -20C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4C as an undiluted liquid. Dilute only prior to immediate use.
• Immunogen: Trypsin 1/PRSS1 Antibody was produced by repeated immunizations with bovine pancreatic trypsin protein purified. (Uniprot: P00760)
• Specificity: Cross-reactivity against Trypsin 1/PRSS1 from other tissues and species may occur but has not been specifically determined.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: PRSS1
• Purity: Multi-step

Applications/Dilutions

• Dilutions:
  • ELISA 1:5000-1:20000
  • Western Blot 1:1000-1:5000
• Application Notes: This product has been tested by western blotting and for ELISA. Researchers should determine optimal titers for applications that are not stated below.

Reactivity Notes

Cross-reactivity against Trypsin 1/PRSS1 from other tissues and species may occur but has not been specifically determined.

Packaging, Storage & Formulations

• Storage: Store lyophilized antibody at 4C. Aliquot reconstituted liquid and store at -20C. Avoid freeze-thaw cycles.
• Buffer: Lyophilized from 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
• Preservative: 0.01% Sodium Azide
• Concentration: LYOPH
• Purity: Multi-step
• Reconstitution Instructions: Reconstitute with 100 ul deionized water (or equivalent)

Alternate Names for Trypsin 1/PRSS1 Antibody - BSA Free

• Beta-trypsin
• Cationic trypsinogen
• digestive zymogen
• DKFZp779A0837
• EC 3.4.21
• EC 3.4.21.4
• FLJ57296
• MGC120175
• MGC149362
• nonfunctional trypsin 1
• protease, serine, 1 (trypsin 1)
• PRSS1
• PTRYI
• Serine protease 1
• TRP1
• TRY1beta-trypsin
• TRY4
• Trygn16
• TRYP1
• Trypsin 1
• Trypsin I
• trypsin-1
• Trypsinogen 1
• trypsinogen A

Background

The Trypsin gene encodes a trypsinogen, which is a member of the trypsin family of serine proteases. This enzyme is secreted by the pancreas and cleaved to its active form in the small intestine. It is active on peptide linkages involving the carboxyl group of l

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: PRSS1
• Uniprot:
  • P00760(Bovine)