Trypsin 1/PRSS1 Antibody (446628)

Protein expression levels of (A) PRSS1 and (B) SPINK1 in AP patients before and after treatment. Patients were divided into three groups based on the severity of their AP; mild, modest and severe. Out of a total of 50 AP patients that underwent ITCM therapy, 26 exhibited mild AP (light grey), 13 exhibited modest AP (medium grey) and 11 patients were diagnosed with severe AP (dark grey). Following ITCM treatment, 29 patients exhibited excellent therapeutic results (26 mild, two modest and one severe), 17 patients exhibited an effective outcome (11 modest and six severe) and four severe patients showed an ineffective response. Data are represented as the mean ± standard deviation of three independent experiments. *P<0.01, vs. value before treatment. AP, acute pancreatitis; ITCM, integrated traditional Chinese medicine. Image collected and cropped by CiteAb from the following open publication (//pubmed.ncbi.nlm.nih.gov/25667658), licensed under a CC-BY license. Not internally tested by R&D Systems.

• Reactivity: Hu
• Applications: WB, IP
• Clone: 446628
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Concentration: LYOPH

Trypsin 1/PRSS1 Antibody (446628) Summary

• Immunogen: Mouse myeloma cell line NS0-derived recombinant human Trypsin 1/PRSS1
  Ala16-Ser247
  Accession # P07477
• Specificity: Detects human Trpysin 1/PRSS1 in direct ELISAs and Western blots. Does not cross-react with recombinant mouse (rm) Trypsin 3, recombinant human (rh) Trypsin 2, or rhTrypsin 3.
• Source: N/A
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Mouse
• Gene: PRSS1
• Purity Statement: Protein A or G purified from hybridoma culture supernatant

Applications/Dilutions

• Dilutions:
  • Immunoprecipitation 25 ug/mL
  • Western Blot 1 ug/mL

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  12 months from date of receipt, -20 to -70 degreesC as supplied. 1 month, 2 to 8 degreesC under sterile conditions after reconstitution. 6 months, -20 to -70 degreesC under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Preservative: No Preservative
• Concentration: LYOPH
• Reconstitution Instructions: Reconstitute at 0.5 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Trypsin 1/PRSS1 Antibody (446628)

• Beta-trypsin
• Cationic trypsinogen
• digestive zymogen
• DKFZp779A0837
• EC 3.4.21
• EC 3.4.21.4
• FLJ57296
• MGC120175
• MGC149362
• nonfunctional trypsin 1
• protease, serine, 1 (trypsin 1)
• PRSS1
• PTRYI
• Serine protease 1
• TRP1
• TRY1beta-trypsin
• TRY4
• Trygn16
• TRYP1
• Trypsin 1
• Trypsin I
• trypsin-1
• Trypsinogen 1
• trypsinogen A

Background

Human Trypsin 1, encoded by the PRSS1 gene, is also known as cationic trypsinogen (1). Constituting approximately two-thirds of the total trypsin content in normal pancreatic juice, it is the most abundant trypsin isoform produced by the pancreas. It contains a signal peptide (residues 1-15), a pro region (residues 16-23), and a mature chain (residues 24-247). Trypsin 1 is synthesized in the pancreas and secreted into the duodenum lumen, where it is activated by enterokinase. Its major physiologic function is to digest food and to activate other pro-enzymes (2). Mutations in the PRSS1 gene can cause hereditary pancreatitis (3).

1. Emi, M. et al. (1986) Gene 41:305.
2. Halfon, S. et al. (2004) in Handbook of Proteolytic Enzymes (ed. Barrett, et al.) p. 1483, Academic Press, San Diego.
3. Teich, N. et al. (2006) Hum. Mutat. 27:721.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Trypsin 1/PRSS1 Antibody (MAB3848)(2)

Publications using MAB3848

• QIANG GAO, NUSHENG LIANG Integrated traditional Chinese medicine improves acute pancreatitis via the downregulation of PRSS1 and SPINK1 Experimental and Therapeutic Medicine 2015-03-01 [PMID: 25667658]
• Lohr JM, Faissner R, Koczan D, Bewerunge P, Bassi C, Brors B, Eils R, Frulloni L, Funk A, Halangk W, Jesenofsky R, Jesnowski R, Kaderali L, Kleeff J, Kruger B, Lerch MM, Losel R, Magnani M, Neumaier M, Nittka S, Sahin-Toth M, Sanger J, Serafini S, Schnolzer M, Thierse HJ, Wandschneider S, Zamboni G, Kloppel G Autoantibodies against the exocrine pancreas in autoimmune pancreatitis: gene and protein expression profiling and immunoassays identify pancreatic enzymes as a major target of the inflammatory process. Am. J. Gastroenterol., 2010-04-20;105(9):2060-71. 2010-04-20 [PMID: 20407433] (Western Blot, Human)

Reviews for Trypsin 1/PRSS1 Antibody (MAB3848) (2)

Average Rating: 5

(Based on 2 reviews)

We have 2 reviews tested in 2 species: Human, Human and Rat.

reviewed by: Scott Messenger WB Human and Rat 05/05/2017

Summary

• Application: Western Blot
• Sample Tested: acini (group of isolated acinar cells) pancreas,AR42J rat acinar pancreatic cancer cell line
• Species: Human and Rat

Comments

• Comments: 50 ug whole cell lysates. antibody 1/1000

reviewed by: Verified Customer ICC Human 02/20/2015

Summary

• Application: Immunocytochemistry
• Sample Tested: See PMID 20407433
• Species: Human

Bioinformatics

• Gene Symbol: PRSS1
• Uniprot:
  • P07477()