TRPC3 Antibody

• Reactivity: Hu, Mu, Rt
• Applications: WB, IHC, IP
• Clonality: Polyclonal
• Host: Goat
• Conjugate: Unconjugated
• Concentration: LYOPH

TRPC3 Antibody Summary

• Description: Novus Biologicals Goat TRPC3 Antibody (NBP1-70352) is a polyclonal antibody validated for use in IHC, WB and IP. Anti-TRPC3 Antibody: Cited in 1 publication. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: A synthetic peptide from amino acid region 780-835 of rat TRPC3 conjugated to an immunogenic carrier protein was used as the antigen.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Gene: TRPC3
• Purity: Unpurified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry 1:300-1:2000
  • Immunohistochemistry-Paraffin 1 : 300 -1 : 2000
  • Immunoprecipitation
  • Western Blot 1:300-1:2000
• Application Notes: Use in immunoprecipitation reported in scientific literature (PMID: 27861908).

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: Lyophilized from whole antisera
• Preservative: No Preservative
• Concentration: LYOPH
• Purity: Unpurified
• Reconstitution Instructions: Reconstitute in 0.1 ml of sterile water. Centrifuge to remove any insoluble material. Glycerol may be added (1:1) for additional stability. Please note the sample size is provided in reconstituted format.

Alternate Names for TRPC3 Antibody

• hTrp3
• hTrp-3
• short transient receptor potential channel 3
• transient receptor potential cation channel, subfamily C, member 3
• transient receptor potential channel 3
• Transient receptor protein 3
• TRP3
• TRP-3
• TRPC3

Background

FUNCTION: Thought to form a receptor-activated non-selective calcium permanent cation channel. Probably is operated by a phosphatidylinositol second messenger system activated by receptor tyrosine kinases or G-protein coupled receptors. Activated by diacylglycerol (DAG) in a membrane-delimited fashion, independently of protein kinase C, and by inositol-1,4,5-triphosphate receptors (ITPR) with bound IP3. May also be activated by internal calcium store depletion. SUBCELLULAR LOCATION: Membrane; Multi-pass membrane protein. TISSUE SPECIFICITY: Abundantly expressed in brain. Concentrated in cerebellar Purkinje cells and sparsely localized in cerebellar granule lyer, pontine nuclei and thalamus. Lower levels detected in other tissues. SIMILARITY: Belongs to the transient receptor family, STrpC subfamily. SIMILARITY: Contains 4 ANK repeats.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for TRPC3 Antibody (NBP1-70352)(1)

Publication using NBP1-70352

• Alvarez-Miguel I, Cidad P, Perez-Garcia MT, Lopez-Lopez JR. Differences in TRPC3 and TRPC6 channels assembly in mesenteric vascular smooth muscle cells in essential hypertension. J. Physiol. (Lond.). 2016-11-12 [PMID: 27861908] (IP, Mouse)

FAQs for TRPC3 Antibody (NBP1-70352). (Showing 1 - 1 of 1 FAQ).

1. I would like to test some of your TRPC3 antibodies in my model system (Xenopus), but they have not been proven to work in this species. Do you offer any guarantee, or beta testing program. We would be happy to send you any images we produce if the antibody works (WB, IHC, ICC). We know that TRPC1-5 are expressed in our system (Xenopus spinal cord).
   • We do offer a testing program, the Innovators Reward Program. Our Innovator’s Reward program is designed to support your innovative research with minimal financial risk to you. Should you decide to use one of our products in an application or species for which it has not been tested all you need to do is submit a full review of the antibody with an image to qualify.

Bioinformatics

• Gene Symbol: TRPC3