TROY/TNFRSF19 Antibody (323606) [Alexa Fluor® 532]

• Applications: WB
• Clone: 323606
• Clonality: Monoclonal
• Host: Rat
• Conjugate: Alexa Fluor 532

TROY/TNFRSF19 Antibody (323606) [Alexa Fluor® 532] Summary

• Immunogen: S. frugiperda insect ovarian cell line Sf 21-derived recombinant mouse TROY/TNFRSF19
  Glu30-Leu170
  Accession # Q9JLL3
• Specificity: Detects mouse TROY/TNFRSF19 in direct ELISAs and Western blots. In Western blots, no cross-reactivity with recombinant mouse (rm) 4‑1BB, rmBAFF R, recombinant human RELT, rmCD3, rhDR6, rmDR3, rhTRADD, rmEDAR, recombinant rat FAS, rmFAS, rmGITR, rmHVE
• Isotype: IgG2a
• Clonality: Monoclonal
• Host: Rat
• Purity Statement: Protein A or G purified

Packaging, Storage & Formulations

• Storage: Protect from light. Do not freeze. 12 months from date of receipt, 2 to 8 °C as supplied
• Buffer: Supplied 0.2mg/ml in 1X PBS with RDF1 and 0.09% Sodium Azide

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Background

TROY (also known as TAJ and TNFRSF19) is a 45‑55 kDa member of the TNF receptor superfamily of molecules (1‑4). It is widely expressed in the embryo, but shows limited expression in adult. In particular, embryonic TROY is found in embryonic epithelium, neural tube, and mesenchyme, and developing neurons of the hippocampus and thalamus (5, 6). In the adult, TROY is found in the nervous system associated with dorsal root and retinal ganglia neurons, astrocytes, and microglia, and in hair follicles where it is expressed by dermal papilla matrix cells (4, 7, 8, 9). In human, TROY has also been found on melanoma cells, not normal melanocytes (10). TROY is reportedly a component of the Nogo-A receptor, taking the place of p75 in a membrane-bound LINGO-1:NgR1:TROY complex (6  8, 9). This complex purportedly mediates axon repulsion initiated by one of five myelin-associated inhibitory factors (OMpg; CD100; Ephrin B3; Nogo-A; MAG) (6, 11). In the hair follicle, TROY binds LTa and acts with Eda to drive secondary hair follicle development (4, 12). Mature human TROY is a 394 amino acid (aa) type I transmembrane protein (1‑3). It possesses a 141 aa extracellular region (aa 30‑170) that contains three 40 aa TNFR cysteine motifs, and an extended 232 aa cytoplasmic domain (aa 192‑423). There is one potential isoform that shows a two aa substitution for aa 416 ‑ 423. The extracellular domains of human and mouse TROY share 90% aa identity.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.