Troponin I Type 1 (slow skeletal) Antibody

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Western Blot: Troponin I Type 1 (slow skeletal) Antibody [NBP2-20733] - Sample (30 ug of whole cell lysate) A: Raji 12% SDS PAGE gel, diluted at 1:1000.

Product Details

Summary
Reactivity Hu, Mu, Rt, Po, Bv, MkSpecies Glossary
Applications WB
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Concentration
1 mg/ml

Order Details

Troponin I Type 1 (slow skeletal) Antibody Summary

Immunogen
Recombinant protein encompassing a sequence within the center region of human Troponin I. The exact sequence is proprietary.
Predicted Species
Mouse (98%), Rat (97%), Porcine (98%), Bovine (97%), Monkey (100%). Backed by our 100% Guarantee.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
TNNI1
Purity
Immunogen affinity purified
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Applications/Dilutions

Dilutions
  • Western Blot 1:500-1:3000
Theoretical MW
22 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Reactivity Notes

Rhesus Monkey (100%).

Packaging, Storage & Formulations

Storage
Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Buffer
PBS (pH 7.0), 1.0% BSA and 20% Glycerol
Preservative
0.01% Thimerosal
Concentration
1 mg/ml
Purity
Immunogen affinity purified

Alternate Names for Troponin I Type 1 (slow skeletal) Antibody

  • DKFZp451O223
  • slow-twitch isoform
  • SSTNI
  • TNN1
  • troponin I type 1 (skeletal, slow)
  • troponin I, skeletal, slow
  • troponin I, slow skeletal muscle

Background

Troponin proteins associate with tropomyosin and regulate the calcium sensitivity of the myofibril contractile apparatus of striated muscles. Troponin I (TnI), along with troponin T (TnT) and troponin C (TnC), is one of 3 subunits that form the troponin complex of the thin filaments of striated muscle. TnI is the inhibitory subunit; blocking actin-myosin interactions and thereby mediating striated muscle relaxation. The TnI subfamily contains three genes: TnI-skeletal-fast-twitch, TnI-skeletal-slow-twitch, and TnI-cardiac. The TnI-fast and TnI-slow genes are expressed in fast-twitch and slow-twitch skeletal muscle fibers, respectively, while the TnI-cardiac gene is expressed exclusively in cardiac muscle tissue. This gene encodes the Troponin-I-skeletal-slow-twitch protein. This gene is expressed in cardiac and skeletal muscle during early development but is restricted to slow-twitch skeletal muscle fibers in adults. The encoded protein prevents muscle contraction by inhibiting calcium-mediated conformational changes in actin-myosin complexes. [provided by RefSeq]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Blogs on Troponin I Type 1 (slow skeletal).

Troponin I Type 1 - Find me in your slow-twitch skeletal muscles
Troponin I is part of a heteromeric thin filament regulatory complex that is responsible for modulating skeletal and cardiac muscle contraction. Troponin complex consists of three subunits: troponin I (TnI), troponin T (TnT), and troponin C (TnC). Eac...  Read full blog post.

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Bioinformatics

Gene Symbol TNNI1
Uniprot