Troponin I Type 1 (slow skeletal) Antibody - BSA Free

Immunohistochemistry-Paraffin: Troponin I Type 1 (slow skeletal) Antibody [NBP1-90923] - Staining of human Skeletal muscle shows strong cytoplasmic positivity in myocytes.

Immunohistochemistry-Paraffin: Troponin I Type 1 (slow skeletal) Antibody [NBP1-90923] - Staining of human skeletal muscle shows distinct cytoplasmic positivity in subsets of myocytes.

Immunohistochemistry-Paraffin: Troponin I Type 1 (slow skeletal) Antibody [NBP1-90923] - Staining of human Endometrium shows no cytoplasmic positivity in glandular cells as expected.

Immunohistochemistry-Paraffin: Troponin I Type 1 (slow skeletal) Antibody [NBP1-90923] - Staining of human Liver shows no cytoplasmic positivity in hepatocytes as expected.

Immunohistochemistry-Paraffin: Troponin I Type 1 (slow skeletal) Antibody [NBP1-90923] - Staining of human Stomach shows no cytoplasmic positivity in glandular cells as expected.

• Reactivity: Hu, Mu, Rt
• Applications: WB, ICC/IF, IHC
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: BSA Free

Troponin I Type 1 (slow skeletal) Antibody - BSA Free Summary

• Immunogen: This antibody was developed against Recombinant Protein corresponding to amino acids: PEVERKPKITASRKLLLKSLMLAKAKECWEQEHEEREAEKVRYLAERIPTLQTRGLSLSALQDLCREL
• Predicted Species: Rat (96%). Backed by our 100% Guarantee.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: TNNI1
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence
  • Immunohistochemistry 1:10-1:500
  • Immunohistochemistry-Paraffin 1:50-1:200
  • Western Blot 1:100-1:500
• Application Notes: For IHC-Paraffin, HIER pH 6 retrieval is recommended. ICC/IF, WB, IHC-P reactivity reported in (PMID: 27035161).
• Control Peptide:

  Troponin I Type 1 (slow skeletal) Protein (NBP1-90923PEP)

Reactivity Notes

Reactivity reported in scientific literature (PMID: 23435261). Mouse reactivity reported in scientific literature (PMID: 27035161).

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS (pH 7.2) and 40% Glycerol
• Preservative: 0.02% Sodium Azide
• Purity: Immunogen affinity purified

Alternate Names for Troponin I Type 1 (slow skeletal) Antibody - BSA Free

• DKFZp451O223
• slow-twitch isoform
• SSTNI
• TNN1
• troponin I type 1 (skeletal, slow)
• troponin I, skeletal, slow
• troponin I, slow skeletal muscle

Background

Troponin proteins associate with tropomyosin and regulate the calcium sensitivity of the myofibril contractile apparatus of striated muscles. Troponin I (TnI), along with troponin T (TnT) and troponin C (TnC), is one of 3 subunits that form the troponin complex of the thin filaments of striated muscle. TnI is the inhibitory subunit; blocking actin-myosin interactions and thereby mediating striated muscle relaxation. The TnI subfamily contains three genes: TnI-skeletal-fast-twitch, TnI-skeletal-slow-twitch, and TnI-cardiac. The TnI-fast and TnI-slow genes are expressed in fast-twitch and slow-twitch skeletal muscle fibers, respectively, while the TnI-cardiac gene is expressed exclusively in cardiac muscle tissue. This gene encodes the Troponin-I-skeletal-slow-twitch protein. This gene is expressed in cardiac and skeletal muscle during early development but is restricted to slow-twitch skeletal muscle fibers in adults. The encoded protein prevents muscle contraction by inhibiting calcium-mediated conformational changes in actin-myosin complexes. (provided by RefSeq)

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Troponin I Type 1 (slow skeletal) Antibody (NBP1-90923)(3)

Publications using NBP1-90923

• Park S, Choi Y, Kwak G et al. Application of differentiated human tonsil-derived stem cells to trembler-J mice Muscle Nerve 2017-08-10 [PMID: 28796340] (Human)
• Park S, Choi Y, Jung N et al. Myogenic differentiation potential of human tonsil-derived mesenchymal stem cells and their potential for use to promote skeletal muscle regeneration. Int. J. Mol. Med. 2016-05-01 [PMID: 27035161] (IHC-P, WB, ICC/IF, Human, Mouse)
• Stadler C, Rexhepaj E, Singan VR et al. Immunofluorescence and fluorescent-protein tagging show high correlation for protein localization in mammalian cells. Nat Methods 2013-04-01 [PMID: 23435261]

Bioinformatics

• Gene Symbol: TNNI1