TRIM72 Antibody

Western Blot: TRIM72 Antibody [NBP1-52104] - Staining of mouse heart lysate (35 ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

Immunohistochemistry-Paraffin: TRIM72 Antibody [NBP1-52104] - Staining of paraffin embedded human skeletal muscle. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.

• Reactivity: Hu, Mu, Rt, Bv, Ca
• Applications: WB, ELISA, IHC
• Clonality: Polyclonal
• Host: Goat
• Conjugate: Unconjugated
• Concentration: 0.5 mg/ml

TRIM72 Antibody Summary

• Description: Novus Biologicals Goat TRIM72 Antibody (NBP1-52104) is a polyclonal antibody validated for use in IHC, WB and ELISA. Anti-TRIM72 Antibody: Cited in 1 publication. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: Peptide with sequence C-RPQALSTNLQ corresponding to internal region according to NP_001008275.1.
• Epitope: C-RPQALSTNLQ
• Predicted Species: Rat (100%), Canine (100%), Bovine (100%). Backed by our 100% Guarantee.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Gene: TRIM72
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry 3 - 5 ug/ml
  • Immunohistochemistry-Paraffin
  • Peptide ELISA Detection limit 1:16000
  • Western Blot 0.01 - 0.03 ug/ml
• Application Notes: WB: Approx. 52 kDa band observed in mouse heart lysates (calculated MW of 52.8 kDa band according to mouse NP_001073401.1). IHC: Human skeletal muscle shows staining of the fibre membranes, detached artificially by the sectioning.

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA
• Preservative: 0.02% Sodium Azide
• Concentration: 0.5 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for TRIM72 Antibody

• MG53Mg53
• Mitsugumin-53
• tripartite motif containing 72
• tripartite motif-containing 72
• tripartite motif-containing protein 72

Background

Muscle-specific protein that plays a central role in cell membrane repair by nucleating the assembly of therepair machinery at injury sites. Specifically binds phosphatidylserine. Acts as a sensor of oxidation: upon membranedamage, entry of extracellular oxidative environment results in disulfide bond formation and homooligomerization atthe injury site. This oligomerization acts as a nucleation site for recruitment of TRIM72-containing vesicles to theinjury site, leading to membrane patch formation. Probably acts upstream of the Ca(2+)-dependent membrane resealingprocess. Required for transport of DYSF to sites of cell injury during repair patch formation. Regulates membranebudding and exocytosis. May be involved in the regulation of the mobility of KCNB1-containing endocytic vesicles (Bysimilarity)

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for TRIM72 Antibody (NBP1-52104)(1)

Publication using NBP1-52104

• Park EY, Kwon OB, Jeong BC et al. Crystal structure of PRY-SPRY domain of human TRIM72. Proteins 2010-02-15 [PMID: 19967786]

Bioinformatics

• Gene Symbol: TRIM72
• Entrez:
  • 493829(Human)
  • 434246(Mouse)
  • 365377(Rat)
• Uniprot:
  • NP_001008275.1(Human)