TRIM5 Antibody

Images

 
Western Blot: TRIM5 Antibody [NB100-1226] - (2ug/ml) staining of Human Placenta lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
Immunohistochemistry-Paraffin: TRIM5 Antibody [NB100-1226] - (1ug/ml) staining of paraffin embedded Human Tonsil. Microwaved antigen retrieval with citrate buffer pH 6, HRP-staining. A) positive cells next to a ...read more

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, IHC, IHC-P, PEP-ELISA
Clonality
Polyclonal
Host
Goat
Conjugate
Unconjugated
Concentration
0.5 mg/ml

Order Details

TRIM5 Antibody Summary

Immunogen
Peptide with sequence ASGILVNVKEEVTC corresponding to N-Terminus according to NP_149023.1, NP_149083.1, NP_149084.1.
Specificity
This antibody is expected to recognise all three human isoforms of this protein.
Isotype
IgG
Clonality
Polyclonal
Host
Goat
Gene
TRIM5
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 2 - 6 ug/ml
  • Immunohistochemistry 1:10 - 1:500
  • Immunohistochemistry-Paraffin 1 ug/ml
  • Peptide ELISA Detection limit 1:16000
Application Notes
WB: Approx. 38 kDa band observed in human Plancenta and human uterus lysates (calculated MW of 37.7 kDa band according to NP_149084.1). IHC successful usage on human tonsil. Data obtained from a previous batch (different goat).
Publications
Read Publication using NB100-1226.

Packaging, Storage & Formulations

Storage
Store at -20C. Avoid freeze-thaw cycles.
Buffer
0.5 mg/ml Tris (pH 7.3) and 0.5% BSA
Preservative
0.02% Sodium Azide
Concentration
0.5 mg/ml
Purity
Immunogen affinity purified

Alternate Names for TRIM5 Antibody

  • EC 6.3.2
  • EC 6.3.2.-
  • RING finger protein 88
  • RNF88
  • RNF88tripartite motif protein TRIM
  • TRIM5
  • TRIM5alpha
  • tripartite motif containing 5
  • tripartite motif protein TRIM5
  • tripartite motif-containing 5
  • tripartite motif-containing protein 5

Background

The protein encoded by this gene is a member of the tripartite motif (TRIM) family. The TRIM motif includes three zinc-binding domains, a RING, a B-box type 1 and a B-box type 2, and a coiled-coil region. The protein localizes to cytoplasmic bodies. Its function has not been identified. Five alternatively spliced transcript variants for this gene have been described. However, the full length nature of two of the variants has not been determined.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for TRIM5 Antibody (NB100-1226)(1)


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Video Protocols

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Bioinformatics

Gene Symbol TRIM5
Entrez
Uniprot