TRIM5 Antibody

Western Blot: TRIM5 Antibody [NB100-1226] - Staining of Human Thymus lysate (35 ug protein in RIPA buffer) with (B) and without (A) blocking with the immunizing peptide. Antibody at 1 ug/mL. Detected by chemiluminescence.

Immunohistochemistry-Paraffin: TRIM5 Antibody [NB100-1226] - Negative Control showing staining of paraffin embedded Human Colon, with no primary antibody.

Immunohistochemistry-Paraffin: TRIM5 Antibody [NB100-1226] - Staining of paraffin embedded Human Colon. Antibody at 3 ug/mL. Heat induced antigen retrieval with citrate buffer pH 6, HRP-staining.

• Reactivity: Hu
• Applications: WB, ELISA, IHC
• Clonality: Polyclonal
• Host: Goat
• Conjugate: Unconjugated
• Concentration: 0.5 mg/ml

TRIM5 Antibody Summary

• Immunogen: Peptide with sequence ASGILVNVKEEVTC corresponding to N-Terminus according to NP_149023.1, NP_149083.1, NP_149084.1.
• Specificity: This antibody is expected to recognise all three human isoforms of this protein.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Gene: TRIM5
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry 3 ug/mL
  • Immunohistochemistry-Paraffin 3 ug/mL
  • Peptide ELISA Detection limit 1:16000
  • Western Blot 1 - 3 ug/mL

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA
• Preservative: 0.02% Sodium Azide
• Concentration: 0.5 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for TRIM5 Antibody

• EC 6.3.2
• EC 6.3.2.-
• RING finger protein 88
• RNF88
• RNF88tripartite motif protein TRIM
• TRIM5
• TRIM5alpha
• tripartite motif containing 5
• tripartite motif protein TRIM5
• tripartite motif-containing 5
• tripartite motif-containing protein 5

Background

The protein encoded by this gene is a member of the tripartite motif (TRIM) family. The TRIM motif includes three zinc-binding domains, a RING, a B-box type 1 and a B-box type 2, and a coiled-coil region. The protein localizes to cytoplasmic bodies. Its function has not been identified. Five alternatively spliced transcript variants for this gene have been described. However, the full length nature of two of the variants has not been determined.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for TRIM5 Antibody (NB100-1226)(1)

Publication using NB100-1226

• Reymond A, Meroni G, Fantozzi A et al. The tripartite motif family identifies cell compartments. EMBO J 2001-05-01 [PMID: 11331580]

Bioinformatics

• Gene Symbol: TRIM5
• Entrez:
  • 85363(Human)
• Uniprot:
  • NP_149023(Human)