Trim22 Antibody (OTI1D11) - Azide and BSA Free

Images

 
Western Blot: Trim22 Antibody (OTI1D11) - Azide and BSA Free [NBP2-74608] - Analysis of HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY TRIM22.
Immunocytochemistry/ Immunofluorescence: Trim22 Antibody (OTI1D11) - Azide and BSA Free [NBP2-74608] - Analysis of COS7 cells transiently transfected by pCMV6-ENTRY TRIM22.
Immunohistochemistry: Trim22 Antibody (OTI1D11) - Azide and BSA Free [NBP2-74608] - Analysis of Human tonsil tissue. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min)
Immunohistochemistry: Trim22 Antibody (OTI1D11) - Azide and BSA Free [NBP2-74608] - Analysis of Carcinoma of Human thyroid tissue. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min)
Immunohistochemistry: Trim22 Antibody (OTI1D11) - Azide and BSA Free [NBP2-74608] - Analysis of Human colon tissue. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min)
Immunohistochemistry: Trim22 Antibody (OTI1D11) - Azide and BSA Free [NBP2-74608] - Analysis of Human endometrium tissue. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min)

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, ICC/IF, IHC
Clone
OTI1D11
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Format
Azide and BSA Free
Concentration
LYOPH

Order Details

Trim22 Antibody (OTI1D11) - Azide and BSA Free Summary

Immunogen
Human recombinant protein fragment corresponding to amino acids 61-406 of human TRIM22(NP_006065) produced in E.coli.
Isotype
IgG1
Clonality
Monoclonal
Host
Mouse
Gene
TRIM22
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:500
  • Immunocytochemistry/Immunofluorescence 1:100
  • Immunohistochemistry 1:150
Theoretical MW
56.8 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

Storage
Store at -20C. Avoid freeze-thaw cycles.
Buffer
Lyophilized from PBS (pH 7.3) with 8% Trehalose
Preservative
No Preservative
Concentration
LYOPH
Purity
Immunogen affinity purified
Reconstitution Instructions
Reconstitute with PBS (pH 7.3). To use this carrier-free antibody for conjugation experiments, another round of desalting is highly recommended.

Alternate Names for Trim22 Antibody (OTI1D11) - Azide and BSA Free

  • 50 kDa-stimulated trans-acting factor
  • E3 ubiquitin-protein ligase TRIM22
  • EC 6.3.2
  • EC 6.3.2.-
  • GPSTAF50
  • RING finger protein 94
  • RNF94stimulated trans-acting factor (50 kDa)
  • Staf-50
  • STAF50staf-50
  • tripartite binding motif 22
  • tripartite motif containing 22
  • tripartite motif protein TRIM22
  • tripartite motif-containing 22
  • Tripartite motif-containing protein 22

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

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Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for Trim22 Antibody (NBP2-74608) (0)

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Secondary Antibodies

 

Isotype Controls

Other Available Formats

Alexa Fluor 350 NBP2-74608AF350
Alexa Fluor 405 NBP2-74608AF405
Alexa Fluor 488 NBP2-74608AF488
Alexa Fluor 532 NBP2-74608AF532
Alexa Fluor 594 NBP2-74608AF594
Alexa Fluor 647 NBP2-74608AF647
Alexa Fluor 700 NBP2-74608AF700
Alexa Fluor 750 NBP2-74608AF750
Biotin NBP2-74608B
DyLight 350 NBP2-74608UV
DyLight 405 NBP2-74608V
DyLight 488 NBP2-74608G
DyLight 550 NBP2-74608R
DyLight 594 NBP2-74608DL594
DyLight 650 NBP2-74608C
DyLight 680 NBP2-74608FR
DyLight 755 NBP2-74608IR
FITC NBP2-74608F
HRP NBP2-74608H
Janelia Fluor 549 NBP2-74608JF549
Janelia Fluor 646 NBP2-74608JF646

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Bioinformatics

Gene Symbol TRIM22