TRIM21/SSA1 Antibody

Western Blot: TRIM21/SSA1 Antibody [NB100-1209] - HEK293 lysate (10ug protein in RIPA buffer) overexpressing Human TRIM21 with C-terminal MYC tag probed (1ug/ml) in Lane A and probed with anti-MYC Tag (1/1000) in lane C. Mock-transfected HEK293 probed (1mg/ml) in Lane B.

• Reactivity: Hu
• Applications: WB, ELISA
• Clonality: Polyclonal
• Host: Goat
• Conjugate: Unconjugated
• Concentration: 0.5 mg/ml

TRIM21/SSA1 Antibody Summary

• Immunogen: Peptide with sequence CPLNIGSQGSTDY corresponding to C-Terminus according to NP_003132.2.
• Localization: Cytoplasmic and nuclear.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Gene: TRIM21
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Peptide ELISA Detection limit 1:16000
  • Western Blot 0.2 - 1 ug/ml
• Application Notes: WB: In transfected HEK293 transiently expressing full-length human TRIM21 (myc and DYKDDDDK tagged), a band of approx. 60 kDa band was observed. No bands were observed in mock-transfected HEK293 and the same band was observed using anti-myc tag antibody.
• Control:

  TRIM21/SSA1 Overexpression Lysate

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA
• Preservative: 0.02% Sodium Azide
• Concentration: 0.5 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for TRIM21/SSA1 Antibody

• 52 kDa ribonucleoprotein autoantigen Ro/SS-A
• 52 kDa Ro/SSA
• E3 ubiquitin-protein ligase TRIM21
• EC 6.3.2.-
• rhTRIM21
• RNF81
• RNF81RING finger protein 81
• Ro(SS-A)
• Ro52
• RO52SS-A
• Sicca syndrome antigen A
• Sjoegren syndrome type A antigen
• Sjogren syndrome antigen A1 (52kDa, ribonucleoprotein autoantigen SS-A/Ro)
• SSA1
• SSA1SSA
• TRIM21
• tripartite motif containing 21,52 kDa Ro protein
• tripartite motif-containing 21
• Tripartite motif-containing protein 21

Background

The protein encoded by this gene is a member of the tripartite motif (TRIM) family. The TRIM motif includes three zinc-binding domains, a RING, a B-box type 1 and a B-box type 2, and a coiled-coil region. This protein is part of the RoSSA ribonucleoprotein which includes a single polypeptide and one of four small RNA molecules. The RoSSA particle localizes to both the cytoplasm and the nucleus. RoSSA interacts with autoantigens in patients with Sjogren syndrome and systemic lupus erythematosus. The function of the RoSSA particle has not been determined. Two alternatively spliced transcript variants for this gene have been described; however, the full length nature of one variant has not been determined.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for TRIM21/SSA1 Antibody (NB100-1209)(1)

Publication using NB100-1209

• Chan EK, Di Donato F, Hamel JC et al. 52-kD SS-A/Ro: genomic structure and identification of an alternatively spliced transcript encoding a novel leucine zipper-minus autoantigen expressed in fetal and adult heart. J Exp Med 1995-10-01 [PMID: 7561701]

Bioinformatics

• Gene Symbol: TRIM21
• Entrez:
  • 6737(Human)
• Uniprot:
  • NP_003132.(Human)