TRIM21/SSA1 Antibody

Images

 
Western Blot: TRIM21/SSA1 Antibody [NB100-1209] - HEK293 lysate (10ug protein in RIPA buffer) overexpressing Human TRIM21 with C-terminal MYC tag probed (1ug/ml) in Lane A and probed with anti-MYC Tag (1/1000) in lane ...read more

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, PEP-ELISA
Clonality
Polyclonal
Host
Goat
Conjugate
Unconjugated
Concentration
0.5 mg/ml

Order Details

TRIM21/SSA1 Antibody Summary

Immunogen
Peptide with sequence CPLNIGSQGSTDY corresponding to C-Terminus according to NP_003132.2.
Localization
Cytoplasmic and nuclear.
Isotype
IgG
Clonality
Polyclonal
Host
Goat
Gene
TRIM21
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 0.2 - 1 ug/ml
  • Peptide ELISA Detection limit 1:16000
Application Notes
WB: In transfected HEK293 transiently expressing full-length human TRIM21 (myc and DYKDDDDK tagged), a band of approx. 60 kDa band was observed. No bands were observed in mock-transfected HEK293 and the same band was observed using anti-myc tag antibody.
Positive Control
TRIM21/SSA1 Lysate (NBL1-17281)
Publications
Read Publication using NB100-1209.

Packaging, Storage & Formulations

Storage
Store at -20C. Avoid freeze-thaw cycles.
Buffer
0.5 mg/ml Tris (pH 7.3) and 0.5% BSA
Preservative
0.02% Sodium Azide
Concentration
0.5 mg/ml
Purity
Immunogen affinity purified

Notes

Please note, this antibody is considered Innovators Grade. Innovators Grade antibodies are generally unvalidated and require additional characterization for most new species/applications. Novus has made these antibodies available through our Innovators Reward program. Complete an online review with image, detailing your positive or negative results. In return, you receive a discount voucher for 100% of the purchase price of the reviewed product. Please contact us at innovators@novusbio.com for more details.

Alternate Names for TRIM21/SSA1 Antibody

  • 52 kDa ribonucleoprotein autoantigen Ro/SS-A
  • 52 kDa Ro/SSA
  • E3 ubiquitin-protein ligase TRIM21
  • EC 6.3.2.-
  • RNF81
  • RNF81RING finger protein 81
  • ro(SS-A)
  • Ro52
  • RO52SS-A
  • Sicca syndrome antigen A
  • Sjoegren syndrome type A antigen
  • Sjogren syndrome antigen A1 (52kDa, ribonucleoprotein autoantigen SS-A/Ro)
  • SSA1
  • SSA1SSA
  • TRIM21
  • tripartite motif containing 21,52 kDa Ro protein
  • tripartite motif-containing 21
  • Tripartite motif-containing protein 21

Background

The protein encoded by this gene is a member of the tripartite motif (TRIM) family. The TRIM motif includes three zinc-binding domains, a RING, a B-box type 1 and a B-box type 2, and a coiled-coil region. This protein is part of the RoSSA ribonucleoprotein which includes a single polypeptide and one of four small RNA molecules. The RoSSA particle localizes to both the cytoplasm and the nucleus. RoSSA interacts with autoantigens in patients with Sjogren syndrome and systemic lupus erythematosus. The function of the RoSSA particle has not been determined. Two alternatively spliced transcript variants for this gene have been described; however, the full length nature of one variant has not been determined.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Customers Who Viewed This Item Also Viewed...

NBP1-48002
Species: Hu, Ca, Pm
Applications: WB, ICC/IF, IHC, IHC-P, IP
NB600-101
Species: Hu, Mu, Rt, Bv, Ha, Pm
Applications: WB, Simple Western, B/N, DB, EM, ICC/IF, IHC, IHC-P, IP, PA
NBP1-86998
Species: Hu
Applications: WB, IHC, IHC-P, IP
NBP1-81696
Species: Hu, Mu, Rt
Applications: WB, ICC/IF, IHC, IHC-P
NBP2-57100
Species: Hu
Applications: ICC/IF
MAB2358
Species: Hu, Mu
Applications: IHC, ICC
NBP2-03433
Species: Hu, Ca
Applications: WB, Flow, ICC/IF, IHC, IHC-P
NBP2-34234
Species: Hu
Applications: ICC/IF, IHC-Fr, IHC-P
NB200-540
Species: Mu
Applications: WB, Flow, IA, ICC/IF, IHC, IHC-Fr, IHC-P, IP, CyTOF-ready, Flow-IC
NB500-526
Species: Hu
Applications: WB, ELISA, IHC, IHC-P
NBP2-31898
Species: Hu
Applications: ICC/IF, IHC, IHC-P
NB600-41532
Species: Mu
Applications: WB, ELISA, ICC/IF, IHC, IHC-P
NBP1-89649
Species: Hu
Applications: WB, ICC/IF, IHC, IHC-P
NBP1-87914
Species: Hu, Mu, Rt
Applications: WB, ICC/IF, IHC, IHC-P
NBP1-57487
Species: Hu
Applications: WB, IHC, IHC-P
NB100-1209
Species: Hu
Applications: WB, PEP-ELISA

Publications for TRIM21/SSA1 Antibody (NB100-1209)(1)

Reviews for TRIM21/SSA1 Antibody (NB100-1209) (0)

There are no reviews for TRIM21/SSA1 Antibody (NB100-1209). By submitting a review you will receive an Amazon e-Gift Card or Novus Product Discount.
  • Review with no image -- $10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen
  • Review with an image -- $25/€18/£15/$25 CAD/¥150 Yuan/¥2500 Yen
  • 1st to review with an image -- $50/€35/£30/$50 CAD/¥300 Yuan/¥5000 Yen

Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol

FAQs for TRIM21/SSA1 Antibody (NB100-1209) (0)

There are no specific FAQs related to this product. Read our general customer & technical service FAQs.

Positive Control Lysate(s)

Secondary Antibodies

 

Isotype Controls

Additional TRIM21/SSA1 Products

Bioinformatics Tool for TRIM21/SSA1 Antibody (NB100-1209)

Discover related pathways, diseases and genes to TRIM21/SSA1 Antibody (NB100-1209). Need help? Read the Bioinformatics Tool Guide for instructions on using this tool.
Visit Tool

Diseases for TRIM21/SSA1 Antibody (NB100-1209)

Discover more about diseases related to TRIM21/SSA1 Antibody (NB100-1209).
 

Pathways for TRIM21/SSA1 Antibody (NB100-1209)

View related products by pathway.

PTMs for TRIM21/SSA1 Antibody (NB100-1209)

Learn more about PTMs related to TRIM21/SSA1 Antibody (NB100-1209).
 

Research Areas for TRIM21/SSA1 Antibody (NB100-1209)

Find related products by research area.

Blogs on TRIM21/SSA1

There are no specific blogs for TRIM21/SSA1, but you can read our latest blog posts.

Customers Who Bought This Also Bought

Contact Information

Product PDFs

Calculators

Concentration Calculator

The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.

=
÷

Review this Product

Be the first to review our TRIM21/SSA1 Antibody and receive a gift card or discount.

Bioinformatics

Gene Symbol TRIM21
Entrez
Uniprot