TRIM21/SSA1 Antibody (672722) [Unconjugated]

TRIM21 was detected in immersion fixed human peripheral blood mononuclear cells (PBMCs) stimulated for 8 hours with 20 ng/mL of Recombinant Human IFN-gamma (285-IF) using Mouse Anti-Human TRIM21 Monoclonal Antibody (Catalog # MAB6219) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 493-conjugated Anti-Mouse IgG Secondary Antibody (green; NL009) and counterstained with DAPI (blue). Specific staining was localized to cell surfaces and cytoplasm. View our protocol for Fluorescent ICC Staining of Non-adherent Cells.

A549 human lung carcinoma cell line lysates were prepared and immunoprecipitation was performed using 2.0 μg of Mouse Anti-Human TRIM21 Monoclonal Antibody (Catalog # MAB6219) pre-coupled to Dynabeads Protein G. Immunoprecipitated TRIM21 was detected in Western Blot with a rabbit TRIM21 antibody used at 1/2000. The Ponceau stained transfer of the blot is shown. SM=4% starting material; UB=4% unbound fraction; IP=immunoprecipitate; HC=antibody heavy chain. Image, protocol and testing courtesy of YCharOS Inc. (ycharos.com).

• Reactivity: Hu
• Applications: IP, ICC/IF
• Clone: 672722
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Concentration: LYOPH

TRIM21/SSA1 Antibody (672722) [Unconjugated] Summary

• Immunogen: E. coli-derived recombinant human TRIM21
  Arg195-Pro293
  Accession # P19474
• Specificity: Detects human TRIM21 in direct ELISAs. In direct ELISAs, no cross-reactivity with recombinant human (rh) TRIM, rhTRIM5, rhTRIM5a, rhTRIM32, rhTRIF (aa 29-204), or rhTRIF (aa 474-618) is observed
• Source: N/A
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Mouse
• Gene: TRIM21
• Purity Statement: Protein A or G purified from hybridoma culture supernatant

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry 8-25 ug/mL
  • Immunoprecipitation 2 ug/mL

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. See Certificate of Analysis for details.
  *Small pack size (-SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Preservative: No Preservative
• Concentration: LYOPH
• Reconstitution Instructions: Sterile PBS to a final concentration of 0.5 mg/mL. For liquid material, refer to CoA for concentration.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for TRIM21/SSA1 Antibody (672722) [Unconjugated]

• 52 kDa ribonucleoprotein autoantigen Ro/SS-A
• 52 kDa Ro/SSA
• E3 ubiquitin-protein ligase TRIM21
• EC 6.3.2.-
• rhTRIM21
• RNF81
• RNF81RING finger protein 81
• ro(SS-A)
• Ro52
• RO52SS-A
• Sicca syndrome antigen A
• Sjoegren syndrome type A antigen
• Sjogren syndrome antigen A1 (52kDa, ribonucleoprotein autoantigen SS-A/Ro)
• SSA1
• SSA1SSA
• TRIM21
• tripartite motif containing 21,52 kDa Ro protein
• tripartite motif-containing 21
• Tripartite motif-containing protein 21

Background

TRIM21 (Tripartite motif-containing protein 21; also Ro(SS-A), 52 kDa Ro Protein/Ro52, and RING finger protein 81) is a 52-56 kDa member of the RING finger-B-box-coiled-coil family of proteins. It is an E3 ligase that is found in both nucleus and cytoplasm, where it is often associated with microtubules. TRIM21 ubiquitinates select proteins. In B cells, it targets the Fc fragment of misfolded IgG, providing QC on its production. In macrophages, it acts in a nondegradative manner on IRF8, promoting innate immunity. Human TRIM21 is 475 amino acids (aa) in length. It contains one E3 ligase RING finger domain (aa 16-55), a B-Box type zinc-finger region (aa 92-123), a coiled-coil region (aa 128-238) and a C-terminal SPRY/B30.2 Ig binding domain (aa 268-465). TRIM21 is reported to form trimers. Over aa 195‑293, human TRIM21 exhibits 72% aa identity with mouse TRIM21.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: TRIM21
• Uniprot:
  • P19474(Human)