TREML1/TLT-1 Antibody

Images

 
There are currently no images for TREML1/TLT-1 Antibody (AF2424).

Every product we sell is backed by Novus' 100% Guarantee. If you have used this product, please submit your images and reviews to earn reward points.

Product Details

Summary
Reactivity MuSpecies Glossary
Applications WB
Clonality
Polyclonal
Host
Goat
Conjugate
Unconjugated
Concentration
LYOPH

Order Details

View Available Conjugates
Catalog# & Conjugate Size Price
View Available Formulations
Catalog# & Formulation Size Price

TREML1/TLT-1 Antibody Summary

Immunogen
Mouse myeloma cell line NS0-derived recombinant mouse TREML1/TLT‑1
Gly18-Cys178
Accession # NP_082039
Specificity
Detects mouse TREML1/TLT‑1 in direct ELISAs and Western blots. In direct ELISAs and Western blots, approximately 10% cross-reactivity with recombinant human TREML1 is observed.
Source
N/A
Isotype
IgG
Clonality
Polyclonal
Host
Goat
Gene
TREML1
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 0.1 ug/mL

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
Preservative
No Preservative
Concentration
LYOPH
Purity
Immunogen affinity purified
Reconstitution Instructions
Reconstitute at 0.2 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for TREML1/TLT-1 Antibody

  • dJ238O23.3
  • GLTL1825
  • PRO3438
  • TLT1
  • TLT-1
  • TLT1MGC119173
  • TREML1
  • trem-like transcript 1 protein
  • triggering receptor expressed on myeloid cells-like 1
  • Triggering receptor expressed on myeloid cells-like protein 1

Background

Triggering receptor expressed on myeloid cells-like protein 1 (TREML1)/TREM-like transcript-1 (TLT‑1) is a 40 kDa type I transmembrane receptor and member of the TREM family of proteins (1). Mouse TREML1/TLT-1 is synthesized as a 317 amino acid (aa) precursor that contains a 20 aa signal sequence, a 155 aa extracellular domain (ECD), a 21 aa transmembrane region, and a 121 aa cytoplasmic domain (SwissProt #: Q8K558). The ECD contains an Ig-like V-type domain (aa 21‑122), and the cytoplasmic region contains a proline-rich region (aa 245‑275) and an immunoreceptor tyrosine-based inhibition motif (ITIM) characterized by the residues S/I/V/LXYXXV/L (1). A splicing variant produces a second isoform that has a six aa substitution for aa 173 in isoform 1. Mature mouse TREML1/TLT-1 shares 65% aa sequence identity with mature human TREML1/TLT-1. TREML1/TLT-1 is expressed exclusively in megakaryocytes and platelets where it colocalizes with CD62P in alpha ‑granules in resting platelets and on the cell surface of platelets activated by thrombin (1‑3). The receptor’s expression is up‑regulated dramatically upon platelet activation (2). Antibodies to single-chain Fv fragments specific for TLT-1 inhibited thrombin-mediated human platelet aggregation suggesting its role in the regulation of aggregation (5). In addition, soluble fragments including the ECD can be released into serum by proteolysis (3). The phosphorylated TREML1/TLT-1 is able to interact with both SHP-1 and SHP-2 through ITIM (1, 4). SHP-2 interaction enhances Fc epsilon RI-mediated calcium signaling in rat basophilic leukemia cells (1). It is hypothesized that TREML1/TLT-1 plays a role in the innate and adaptive immune responses.
  1. Barrow, A.D. et al. (2004) J. Immunol. 172:5838.
  2. Washington, A.V. et al. (2004) Blood 104:1042.
  3. Gattis, J.L. et al. (2006) J. Biol. Chem. 281:13396.
  4. Washington, A.V. et al. (2002) Blood 100:3822.
  5. Giomarelli B. et al. (2007) Thromb. Haemost. 97:955.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Customers Who Viewed This Item Also Viewed...

MAB1278
Species: Hu
Applications: Flow, AgAct, CyTOF-ready
AF1729
Species: Mu
Applications: WB, ICC
AF1213
Species: Mu
Applications: WB, IHC, Block, ICC
NBP2-67321
Species: Hu, Mu, Rt
Applications: WB, Flow, ICC/IF, IHC, IHC-P, IP
NB200-193
Species: Hu, Mu, Rt, Po, Bv, Ch, Eq
Applications: WB, Simple Western, Flow, ICC/IF, IHC, IHC-Fr, IHC-P
MAB5240
Species: Hu
Applications: WB, CyTOF-ready, ICFlow
NB400-144
Species: Hu, Mu, Rt, Po, Bv, Pm
Applications: WB, ICC/IF, IHC, IHC-Fr, IHC-P
AF6354
Species: Mu
Applications: WB, Flow, CyTOF-ready
NBP2-67584
Species: Hu, Mu, Rt
Applications: WB, Flow, ICC/IF, IHC, IHC-P, IP
NBP1-81858
Species: Hu
Applications: WB, IHC, IHC-P
MAB6758
Species: Mu
Applications: Flow, CyTOF-ready, ICC
MAB2774
Species: Hu
Applications: Flow, CyTOF-ready, ICC
NBP2-59376
Species: Hu, Mu, Rt
Applications: WB, ICC/IF, IHC, IP
AF887
Species: Hu, Mu, Rt
Applications: WB, Simple Western, IHC, CyTOF-ready, ICFlow
AF2800
Species: Mu
Applications: WB, IHC, Block
AF2424
Species: Mu
Applications: WB

Publications for TREML1/TLT-1 Antibody (AF2424) (0)

There are no publications for TREML1/TLT-1 Antibody (AF2424).
By submitting your publication information earn gift cards and discounts for future purchases.

Reviews for TREML1/TLT-1 Antibody (AF2424) (0)

There are no reviews for TREML1/TLT-1 Antibody (AF2424). By submitting a review you will receive an Amazon e-Gift Card or Novus Product Discount.
  • Review with no image -- $10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen
  • Review with an image -- $25/€18/£15/$25 CAD/¥150 Yuan/¥2500 Yen
  • 1st to review with an image -- $50/€35/£30/$50 CAD/¥300 Yuan/¥5000 Yen

Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol

FAQs for TREML1/TLT-1 Antibody (AF2424) (0)

There are no specific FAQs related to this product. Read our general customer & technical service FAQs.

Secondary Antibodies

 

Isotype Controls

Additional Array Products

Bioinformatics Tool for TREML1/TLT-1 Antibody (AF2424)

Discover related pathways, diseases and genes to TREML1/TLT-1 Antibody (AF2424). Need help? Read the Bioinformatics Tool Guide for instructions on using this tool.
Visit Tool

Diseases for TREML1/TLT-1 Antibody (AF2424)

Discover more about diseases related to TREML1/TLT-1 Antibody (AF2424).
 

Pathways for TREML1/TLT-1 Antibody (AF2424)

View related products by pathway.

PTMs for TREML1/TLT-1 Antibody (AF2424)

Learn more about PTMs related to TREML1/TLT-1 Antibody (AF2424).

Blogs on TREML1/TLT-1

There are no specific blogs for TREML1/TLT-1, but you can read our latest blog posts.

Customers Who Bought This Also Bought

Contact Information

Product PDFs

Calculators

Concentration Calculator

The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.

=
÷

Review this Product

Be the first to review our TREML1/TLT-1 Antibody and receive a gift card or discount.

Bioinformatics

Gene Symbol TREML1
Uniprot