Trappin-2/Elafin/Skalp Antibody (TRAB2O)

• Reactivity: Hu
• Applications: WB, ELISA, IHC
• Clone: TRAB2O
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Concentration: 0.1 mg/ml

Trappin-2/Elafin/Skalp Antibody (TRAB2O) Summary

• Immunogen: TRAB2O recognises all known Trappins via the GQDPVK epitope.
• Epitope: TRAB2O recognises all known Trappins via the GQDPVK epitope.
• Specificity: TRAB2O shows strong reaction with human native full-length Elafin/SKALP and a weaker reaction with fully processed Elafin/SKALP (C-terminal 57 aa).
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Mouse
• Gene: PI3
• Purity: Protein G purified

Applications/Dilutions

• Dilutions:
  • Immunoassay
  • Immunohistochemistry-Paraffin
  • Western Blot
• Application Notes: For Immunohistochemistry and Western blotting dilutions to be used depend on detection system applied. It is recommended that users test the reagent and determine their own optimal dilutions. The typical starting working dilution is 1:10. For Western blotting it is recommended to use non-reducing conditions.

Reactivity Notes

The antibody is cross reactive with Trappin family members of other species with GQDPVK epitope.

Packaging, Storage & Formulations

• Storage: Store at 4C.
• Buffer: 0.2 um filtered solution in PBS, 0.1% BSA
• Preservative: 0.02% Sodium Azide
• Concentration: 0.1 mg/ml
• Purity: Protein G purified

Alternate Names for Trappin-2/Elafin/Skalp Antibody (TRAB2O)

• cementoin
• Elafin
• Elastase-specific inhibitor
• ESI
• ESIWAP four-disulfide core domain protein 14
• Peptidase inhibitor 3
• peptidase inhibitor 3, skin-derived
• PI3
• PI-3
• pre-elafin
• protease inhibitor 3, skin-derived (SKALP)
• Protease inhibitor WAP3
• SKALP
• SKALPELAFIN
• Skin-derived antileukoproteinase
• Trappin2
• Trappin-2
• WAP four-disulfide core domain 14
• WAP3MGC13613
• WFDC14elafin

Background

Elafin is an epithelial proteinase inhibitor also known under various other names such as Skin-derived Anti leukoproteinase (SKALP) and Elastase-Specific Inhibitor (ESI). Elafin belongs to the Trappin gene family and was given the systematic name Trappin-2. The Trappin family is defined by a N-terminal transglutaminase substrate domain and a C-terminal four disulphide core. Trappins have been suggested to play a role in the regulation of inflammation and in protection against tissue damage in stratified epithelia. Elafin is an inhibitor of leukocyte elastase and proteinase-3 and is a substrate for transglutaminases. The protein is constitutively expressed in various epithelia including hair follicles, oesophagus, vagina and oral cavity. Elafin is not present in normal human skin but is strongly induced during inflammation as in psoriasis and wound healing. Antibodies to Elafin can be used to evaluate the effects of treatment of psoriasis since its expression is significantly correlated with clinical scores. Antibodies to Elafin have also been succesfully used to study differentiation in squamous cell carcinoma of the head-and-neck region, oesophagus and skin. It is also shown that Elafin possesses antimicrobial activity against gram-positive and gram-negative bacteria. TRAB2O recognises all known Trappins via the GQDPVK epitope. TRAB2O shows strong reaction with human native full-length Elafin/SKALP and a weaker reaction with fully processed Elafin/SKALP (C-terminal 57 aa). For detection of fully processed Elafin/SKALP (C-terminal 57 aa) we advise to use TRAB2F. The antibody is cross reactive with Trappin family members of other species with GQDPVK epitope.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: PI3