Transglutaminase 2/TGM2 Assay Kit (Colorimetric)

ELISA: Transglutaminase 2/TGM2 Assay Kit (Colorimetric) [NBP1-37008] - Standard Curve image from a verified customer review.

• Reactivity: All
• Applications: Func
• Conjugate: HRP

Transglutaminase 2/TGM2 Assay Kit (Colorimetric) Summary

• Description: The Transglutaminase 2/TGM2 Assay Kit uses biotinylated T26 peptide (Biotin-pepT26) as the first substrate (amine-acceptor/acyl-donor) and an amine-donor/acyl-acceptor as a second substrate.
  
  Samples suspected of containing TG2 are incubated with calcium, dithiothreitol (DTT) and Biotin-pepT26 in the wells of microtiter plates containing the amine substrate. In the presence of TG2, the gamma carboxamide of the glutaminyl residue of the biotin-pepT26 is incorporated into the amine substrate to form biotinylated isopetide bound.
  
  The system is coupled to Streptavidin labelled peroxidase (SAv-HRP). SAv-HRP is revealed using H2O2 as HRP substrate and tetramethyl benzidine as electron acceptor (chromogen).
• Specificity: Measurement of tissue Transglutaminase (tTG/TG2) with low or no interference with other TGs isoforms.
• Kit Type: Assay Kit (Colorimetric)
• Gene: TGM2

Applications/Dilutions

• Dilutions:
  • Functional

Reactivity Notes

Use in Porcine reported in secitific publication PMID: 32431691

Packaging, Storage & Formulations

• Storage: Storage of components varies. See protocol for specific instructions.

Notes

The kit is shipped on ice. Upon arrival, the DTT (R1), the Reaction Buffer (R3) and Enzyme Tracer (R4) should be stored at -20C. All the other components of the kit should be kept at 4C. When stored properly, these stock solutions are stable for at least 24 months. OTHER SUPPLIES REQUIRED: - Recombinant human TG2 - TG2 inhibitor. This is recommended as negative control for screening of TG2 inhibitors.

Alternate Names for Transglutaminase 2/TGM2 Assay Kit (Colorimetric)

• C polypeptide
• EC 2.3.2.13
• G-ALPHA-h
• protein-glutamine gamma-glutamyltransferase 2
• protein-glutamine-gamma-glutamyltransferase
• TG(C)
• TG2
• TGase C
• TGase H
• TGase-2
• TGase-H
• TGC
• TGCGNAH
• TGM2
• Tissue transglutaminase
• transglutaminase 2 (C polypeptide, protein-glutamine-gamma-glutamyltransferase)
• Transglutaminase 2
• Transglutaminase C
• Transglutaminase H
• transglutaminase-2
• tTG

Background

Transglutaminases (EC. 2.3.213, R- glutamyl-peptide amine g-glutamyl-transferase) are a family of calcium dependent enzymes which catalyse an acyl transferase reaction between the gamma-carboxamide group of peptide bound glutamine and various primary amines. In mammals, at least eight active transglutaminase (TGs) isoenzymes have been described so far. They are widely distributed in various organs, tissues and body fluids. Among them, transglutaminase type 2 (TG2) is distinguished from others TGs by its functional versatility and ubiquitous expression pattern in mammalian tissues. This isoenzyme is involved in a variety of roles including stabilization of intra and extracellular matrices and crosslinking of cell envelopes in apoptosis. It has also been associated with a large number of pathological conditions such as fibrosis, celiac disease, neurodegenerative disorders, inflammatory processes in sepsis, and in carcinogenesis of hepatocellular and ovarian carcinoma. This consideration has led to the development of several tests for either research or clinical purpose. Among the various strategies developed to measure TG activity, the most sensitive and accurate quantification methods, although non-discriminatory, have been the radioactive and fluorimetric assays. Those methods detect the incorporation of labeled molecules, such as [3H/14C]-putrescine or monodansyl-cadaverine respectively, into glutamyl substrates such as casein or synthetic peptides. Solid phase assays developed so far in similar principles have been compromise by a high background signal, low sensitivity compare to radiolabeling methods and the lack of the specificity. This test kit overcomes these problems and constitutes a highly sensitive and specific TG2 solid-phase microassay. The TG2-Covtest uses a biotinylated preferred first substrate of TG2 (biotin-pepT26) as amine-acceptor, and spermine as second substrate (amine-donor) of the enzyme. Samples suspected of containing TG2 are incubated with calcium, dithiothreitol (DTT) and biotin-pepT26 in the wells of microtiter plates to which spermine has been covalently coupled. In the presence of TG2, spermine is incorporated into the g carboxamide of the glutaminyl residue of biotin-pepT26 to form a biotin-pepT26- g-glutamyl spermine. Enzymatic reaction is determined by its interaction with Streptavidin labelled peroxidase (SAv-HRP). Following a wash step to remove any unbound enzyme reagent, a substrate solution for SAv-HRP containing H2O2 and tetramethyl benzidine as electron acceptor (chromogen) is added and color developed. The color intensity is directly proportional to the TG2 activity in the sample.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Kits are guaranteed for 6 months from date of receipt.

Publications for Transglutaminase 2/TGM2 Kit (NBP1-37008)(9)

Publications using NBP1-37008

• Doki Y, Nakazawa Y, Sukegawa M et al. Piezo1 channel causes lens sclerosis via transglutaminase 2 activation Experimental eye research 2023-12-01 [PMID: 37951336]
• Panes-Fernandez J, Godoy PA, Gavilan J et al. TG2 promotes amyloid beta aggregates: Impact on ER-mitochondria crosstalk, calcium homeostasis and synaptic function in Alzheimer's disease Biomedicine & pharmacotherapy = Biomedecine & pharmacotherapie 2023-03-27 [PMID: 36989728] (Func, All)
• Thomas K, Zondler L, Ludwig N et al. Glutamine prevents acute kidney injury by modulating oxidative stress and apoptosis in tubular epithelial cells JCI insight 2022-11-08 [PMID: 36107633] (Func, All)
• Han MS, Jung YK, Kim GW, Han S Transglutaminase-2 regulates Wnt and FoxO3a signaling to determine the severity of osteoarthritis Sci Rep 2020-08-06 [PMID: 32764573]
• Chen C, Smith AD, Cheung L et al. Potentiation of IL-4 Signaling by Retinoic Acid in Intestinal Epithelial Cells and Macrophages-Mechanisms and Targets Front Immunol 2020-05-05 [PMID: 32431691] (Porcine)
• Jung HJ, Han EH, Jang IK et al. Effect of Tissue Transglutaminase on Steroid Resistance in T-Cell Acute Lymphoblastic Leukemia Anticancer Res. 2019-11-01 [PMID: 31704844]
• Jung HJ, Chen Z, Wang M et al. Calcium blockers decrease the bortezomib resistance in mantle cell lymphoma via manipulation of tissue transglutaminase activities Blood 2012-03-15 [PMID: 22294726]
• Perez Alea M, Kitamura M, Martin G, Thomas V, Hitomi K, El Alaoui S. Development of an isoenzyme-specific colorimetric assay for tissue transglutaminase 2 cross-linking activity. Anal Biochem. 389(2):150-6. 2009-06-15 [PMID: 19318081]
• Thomas V, El Alaoui S, Massignon D, Clement S, Simonet F, Quash G. Development and evaluation of a modified colorimetric solid-phase microassay for measuring the activity of cellular and plasma (Factor XIII) transglutaminases. Biotechnol Appl Biochem. 43(Pt 3):171-9. 2006-03-01 [PMID: 16313237]

Review for Transglutaminase 2/TGM2 Kit (NBP1-37008) (1)

Average Rating: 5

(Based on 1 review)

reviewed by: Verified Customer 05/16/2025

Summary

• Lot: 24F88

FAQs for Transglutaminase 2/TGM2 Kit (NBP1-37008). (Showing 1 - 3 of 3 FAQs).

1. Does this kit contain recombinants using baculovirus expression system? If there are such recombinants, could you let us know the component name?
   • To my knowledge, this kit does not contain any recombinant proteins. See below for the component list: Microtiter strips with covalently bound spermine (12x 8-wells strips), DTT (0.2 mL), EDTA (Negative Control) (0.5 mL), Reaction Buffer (Biotin-pepT26/CaCl2) 2 vials (Lyophilized powder), Enzyme Tracer (SAv-HRP) (50 uL), Wash Buffer 10X (30 mL), Diluent Buffer 10X (10 mL), HRP Substrate (12 mL), Blocking Reagent (6 mL).
2. I would like to use the Transglutaminase 2 Kit to detect TGM2 activity in cell lysate from mouse tissue. The tissue protein is in RIPA buffer, I am not sure whether RIPA buffer is OK for using this Kit?
   • It is recommended to use lysate samples in Triton buffer with freezing/thawing cycles. Due to the denaturating properties of RIPA, the use of sample in RIPA buffer is not recommended. However, you may perform a cascade dilution of your samples up to 1/100 at least.
3. Does the Transglutaminase 2/TGM2 kit (NBP1-37008) measure total Transglutaminase 2/TGM2 or just active?
   • The Transglutaminase 2/TGM2 kit (NBP1-37008) only detects active TGM2.

Bioinformatics

• Gene Symbol: TGM2