Transglutaminase 1/TGM1 Antibody

Product Discontinued

Transglutaminase 1/TGM1 Antibody Summary

• Immunogen: A 15 residue synthetic peptide derived from a domainfrom human Transglutaminase 1 protein.
• Specificity: This reacts specifically with human Transglutaminase 1 .
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: TGM1
• Purity: Unpurified

Applications/Dilutions

• Dilutions:
  • ELISA
  • Immunocytochemistry/ Immunofluorescence 1:10-1:500
  • Immunohistochemistry 1:100 - 1:1000
  • Immunohistochemistry-Paraffin 1:100 - 1:1000
  • Western Blot 1:200-1:1000
• Application Notes: Transglutaminase 1/TGM1 antibody validated for ICC/IF from a verified customer review.
• Theoretical MW: 89.8 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
• Control:

  Transglutaminase 1/TGM1 Overexpression Lysate

Reactivity Notes

Cynomolgus Monkey reactivity reported in scientific literature (PMID: 22620270).

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: Antiserum
• Preservative: No Preservative
• Concentration: LYOPH
• Purity: Unpurified
• Reconstitution Instructions: Lyophilized powder, reconstituted in distilled water.

Alternate Names for Transglutaminase 1/TGM1 Antibody

• Epidermal TGase
• ICR2
• KTG
• LI
• LI1
• LI1EC 2.3.2.13
• protein-glutamine gamma-glutamyltransferase K
• protein-glutamine-gamma-glutamyltransferase)
• TG(K)
• TGASE
• TGase-1
• TGK
• TGKTGase K
• TGM1
• transglutaminase 1 (K polypeptide epidermal type I
• Transglutaminase 1
• Transglutaminase K
• transglutaminase, keratinocyte
• transglutaminase-1

Background

Tissue transglutaminase is a calcium activated enzyme which catalyzes the cross linking of proteins and the conjugation of polyamines to proteins. The identification of transglutaminase as the main antigen of endomysium antibodies allows a new diagnostic approach to celiac disease (CD). CD, a genetic, immunologically mediated small bowel enteropathy that causes malabsorption, is one of the more common disorders in Western countries.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Transglutaminase 1/TGM1 Antibody (NB100-1844)(4)

Publications using NB100-1844

• Izquierdo-Altarejos P, Cabrera-Pastor A, Gonzalez-King H et Al. Extracellular Vesicles from Hyperammonemic Rats Induce Neuroinflammation and Motor Incoordination in Control Rats Cells 2020-02-28 [PMID: 32121257] (WB, Rat)
• Terazawa S, Mori S, Nakajima H et al. The UVB-Stimulated Expression of Transglutaminase 1 Is Mediated Predominantly via the NFkB Signaling Pathway: New Evidence of Its Significant Attenuation through the Specific Interruption of the p38/MSK1/NFkBp65 Ser276 Axis. PLoS ONE 2015-08-26 [PMID: 26305102] (WB, Human)
• Yuki T, Komiya A, Kusaka A et al. Impaired Tight Junctions Obstruct Stratum Corneum Formation by Altering Polar Lipid and Profilaggrin Processing Journal of Dermatological Science 2012-01-01 [PMID: 23273645] (WB, Human)
• Miller A D, Westmoreland S V, Evangelous N R et al. Acute traumatic spinal cord injury induces glial activation in the cynomolgus macaque (Macaca fascicularis). Journal of Medical Primatology 2012-01-01 [PMID: 22620270] (IF/IHC, Cynomolgus Monkey)

Review for Transglutaminase 1/TGM1 Antibody (NB100-1844) (1)

Average Rating: 4

(Based on 1 review)

We have 1 review tested in 1 species: Human.

reviewed by: Verified Customer ICC Human 09/10/2018

Summary

• Application: Immunocytochemistry
• Sample Tested: Vaginal epithelial cells
• Species: Human
• Lot: 16G10

Bioinformatics

• Gene Symbol: TGM1
• Entrez:
  • 7051(Human)
• Uniprot:
  • P22735(Human)