Transgelin/TAGLN/SM22 alpha Antibody (SR1369) - BSA Free

Western Blot: Transgelin/TAGLN/SM22 alpha Antibody (SR1369) [NBP3-21936] - Western blot analysis using NBP3-21936 in Human stomach lysate.

• Reactivity: Hu
• Applications: WB, IHC
• Clone: SR1369
• Clonality: Monoclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: BSA Free

Transgelin/TAGLN/SM22 alpha Antibody (SR1369) - BSA Free Summary

• Description: Novus Biologicals Rabbit Transgelin/TAGLN/SM22 alpha Antibody (SR1369) - BSA Free (NBP3-21936) is a recombinant monoclonal antibody validated for use in IHC and WB. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Additional Information: Recombinant Monoclonal Antibody
• Immunogen: A synthesized peptide derived from human Transgelin/TAGLN/SM22 alpha (Uniprot #: Q01995)
• Specificity: Detects endogenous levels of total Transgelin/TAGLN/SM22 alpha
• Source: HEK293
• Isotype: IgG
• Clonality: Monoclonal
• Host: Rabbit
• Gene: TAGLN
• Purity: Affinity purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry 1:50-1:200
  • Western Blot 1:500-1:2000
• Theoretical MW: 23 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS, pH 7.4, 150mM NaCl, 50% glycerol.
• Preservative: 0.02% Sodium Azide
• Purity: Affinity purified

Alternate Names for Transgelin/TAGLN/SM22 alpha Antibody (SR1369) - BSA Free

• DKFZp686P11128
• Protein WS3-10
• SM22 alpha
• SM2222 kDa actin-binding protein
• SMCCDKFZp686B01212
• Smooth muscle protein 22-alpha
• TAGLN
• TAGLN1
• transgelin variant 2
• Transgelin
• WS3-10
• WS3-10SM22-alpha

Background

In contrast to fast and slow skeletal muscle cells that fuse and terminally differentiate, smooth muscle cells are able to simultaneously proliferate and express lineage-restricted proteins. One of these proteins, expressed exclusively in smooth muscles, has been referred to as SM22-alpha, a 22 kDa protein with structural similarity to the vertebrate thin filament myofibrillar regulatory protein calponin and the Drosophila muscle protein mp20, neither of which play a direct role in the contractile apparatus.The protein is a transformation and shape-change sensitive actin cross-linking/geling protein found in fibroblasts and smooth muscle. Its expression is down-regulated in many cell lines, and this down-regulation may be an early and sensitive marker for the onset of transformation. A functional role of this protein is unclear.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: TAGLN