Transcription factor E3 Antibody (JE60-60)

Western Blot: Transcription factor E3 Antibody (JE60-60) [NBP3-33005] - Western blot analysis of Transcription factor E3 on different lysates with Rabbit anti-Transcription factor E3 antibody (NBP3-33005) at 1/1,000 dilution. Lane 1: U-2 OS cell lysate (20 ug/Lane) Lane 2: 293T cell lysate (20 ug/Lane) Lane 3: C2C12 cell lysate (20 ug/Lane) Lane 4: Mouse testis tissue lysate (40 ug/Lane) Lane 5: A-172 cell lysate (30 ug/Lane) Lane 6: NIH/3T3 cell lysate (30 ug/Lane) Lane 7: C6 cell lysate (30 ug/Lane) Lane 8: PC-12 cell lysate (30 ug/Lane) Predicted band size: 61 kDa Observed band size: 70/80 kDa Exposure time: 3 minutes; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-33005) at 1/1,000 dilution was used in 5% NFDM/TBST at 4C overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.

Immunohistochemistry: Transcription factor E3 Antibody (JE60-60) [NBP3-33005] - Immunohistochemical analysis of paraffin-embedded human testis tissue with Rabbit anti-Transcription factor E3 antibody (NBP3-33005) at 1/800 dilution. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (NBP3-33005) at 1/800 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

Flow Cytometry: Transcription factor E3 Antibody (JE60-60) [NBP3-33005] - Flow cytometric analysis of U-2 OS cells labeling Transcription factor E3. Cells were fixed and permeabilized. Then stained with the primary antibody (NBP3-33005, 1μg/mL) (red) compared with Rabbit IgG Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a iFluor™ 488 conjugate-Goat anti-Rabbit IgG Secondary antibody at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black).

• Reactivity: Hu, Mu, Rt
• Applications: WB, Flow, IHC
• Clone: JE60-60
• Clonality: Monoclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Concentration: 1 mg/ml

Transcription factor E3 Antibody (JE60-60) Summary

• Description: Novus Biologicals Rabbit Transcription factor E3 Antibody (JE60-60) (NBP3-33005) is a recombinant monoclonal antibody validated for use in IHC, WB and Flow. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Additional Information: Recombinant Monoclonal Antibody
• Immunogen: Recombinant protein within Human Transcription factor E3 aa 150-499 / 575. (Uniprot: P19532)
• Localization: Cytoplasm, Nucleus.
• Source: HEK293
• Isotype: IgG
• Clonality: Monoclonal
• Host: Rabbit
• Gene: TFE3
• Purity: Protein A purified

Applications/Dilutions

• Dilutions:
  • Flow Cytometry 1:1000
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin 1:800
  • Western Blot 1:1000
• Theoretical MW: 61 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: 1*TBS (pH7.4), 0.05% BSA and 40% Glycerol
• Preservative: 0.05% Sodium Azide
• Concentration: 1 mg/ml
• Purity: Protein A purified

Alternate Names for Transcription factor E3 Antibody (JE60-60)

• BHLHE33
• bHLHe33transcription factor E3
• Class E basic helix-loop-helix protein 33
• RCCP2
• TFEA
• transcription factor binding to IGHM enhancer 3
• transcription factor E family, member A
• Transcription factor for IgH enhancer

Background

TFE3, a member of the helix-loop-helix family of transcription factors, binds to the mu-E3 motif of the immunoglobulin heavy-chain enhancer and is expressed in many cell types (Henthorn et al., 1991 [PubMed 1685140]).[supplied by OMIM]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: TFE3