Transcription factor E3 Antibody (1O9E9)

Western Blot: Transcription factor E3 Antibody (7V8M1) [NBP3-15276] - Western blot analysis of extracts of various cell lines, using Transcription factor E3 Rabbit mAb (NBP3-15276) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s.

Immunohistochemistry-Paraffin: Transcription factor E3 Antibody (7V8M1) [NBP3-15276] - Immunohistochemistry of paraffin-embedded human esophageal cancer using Transcription factor E3 Rabbit mAb (NBP3-15276) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Western Blot: Transcription factor E3 Antibody (7V8M1) [NBP3-15276] - Western blot analysis of extracts of HeLa cells, using Transcription factor E3 Rabbit mAb (NBP3-15276) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.

Immunohistochemistry: Transcription factor E3 Antibody (1O9E9) [Transcription factor E3] - Immunohistochemistry analysis of paraffin-embedded Human acinar soft tissue sarcoma using Transcription factor E3 Rabbit mAb at dilution of 1:100 (40x lens). High pressure antigen retrieval performed with 0.01M Tris/EDTA Buffer (pH 9.0) prior to IHC staining.

• Reactivity: Hu, Mu, Rt
• Applications: WB, IHC
• Clone: 1O9E9
• Clonality: Monoclonal
• Host: Rabbit
• Conjugate: Unconjugated

Transcription factor E3 Antibody (1O9E9) Summary

• Description: Novus Biologicals Rabbit Transcription factor E3 Antibody (1O9E9) (NBP3-15276) is a recombinant monoclonal antibody validated for use in IHC and WB. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Additional Information: Recombinant Monoclonal Antibody
• Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 191-440 of human Transcription factor E3 (P19532). LQQARRQQVKQYLSTTLGPKLASQALTPPPGPASAQPLPAPEAAHTTGPTGSAPNSPMALLTIGSSSEKEIDDVIDEIISLESSYNDEMLSYLPGGTTGLQLPSTLPVSGNLLDVYSSQGVATPAITVSNSCPAELPNIKREISETEAKALLKERQKKDNHNLIERRRRFNINDRIKELGTLIPKSSDPEMRWNKGTILKASVDYIRKLQKEQQRSKDLESRQRSLEQANRSLQLRIQELELQAQIHGLP
• Source: HEK293
• Isotype: IgG
• Clonality: Monoclonal
• Host: Rabbit
• Gene: TFE3
• Purity: Affinity purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry 1:50 - 1:200
  • Immunohistochemistry-Paraffin
  • Western Blot 1:500 - 1:1000

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: PBS (pH 7.3), 50% glycerol, 0.05% BSA
• Preservative: 0.02% Sodium Azide
• Purity: Affinity purified

Alternate Names for Transcription factor E3 Antibody (1O9E9)

• BHLHE33
• bHLHe33transcription factor E3
• Class E basic helix-loop-helix protein 33
• RCCP2
• TFEA
• transcription factor binding to IGHM enhancer 3
• transcription factor E family, member A
• Transcription factor for IgH enhancer

Background

TFE3, a member of the helix-loop-helix family of transcription factors, binds to the mu-E3 motif of the immunoglobulin heavy-chain enhancer and is expressed in many cell types (Henthorn et al., 1991 [PubMed 1685140]).[supplied by OMIM]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: TFE3