TRAILR2/TNFRSF10B Antibody (71908) [Allophycocyanin]

MDA-MB-231 human breast cancer cell line was stained with Mouse Anti-Human TRAIL R2/TNFRSF10B APC-conjugated Monoclonal Antibody (Catalog # FAB6311A, filled histogram) or isotype control antibody (Catalog # IC0041A, open histogram). View our protocol for Staining Membrane-associated Proteins.

pHe influences the expression of TRAIL death receptors. (A,B) PDAC cells were grown for 24 h, then stained with APC-conjugated anti-TRAIL-Rs antibodies to measure their cell surface expression by flow cytometry. (C,D) PDAC cells were grown for 24 h, then either not treated (−) or treated (+) with 200 ng/ml TRAIL for 24 h, lysed, and subjected to western blot analyses for TRAIL-R1 and TRAIL-R2. Blots are representative and (E,F) show densitometric quantification normalized to loading control and the respective level of untreated cells cultured under pHe 7.4 conditions. Data are shown as mean with S.E.M error bars, of at least three independent experiments per cell line. **<0.01, ***<0.001, and ****<0.0001: significant difference between untreated and treated cells using two-way ANOVA with Sidak’s multiple-comparison test or between pHe conditions using two-way ANOVA with Tukey’s multiple-comparison test. Image collected and cropped by CiteAb from the following open publication (//pubmed.ncbi.nlm.nih.gov/35281089), licensed under a CC-BY license. Not internally tested by R&D Systems.

pHe influences the expression of TRAIL death receptors. (A,B) PDAC cells were grown for 24 h, then stained with APC-conjugated anti-TRAIL-Rs antibodies to measure their cell surface expression by flow cytometry. (C,D) PDAC cells were grown for 24 h, then either not treated (−) or treated (+) with 200 ng/ml TRAIL for 24 h, lysed, and subjected to western blot analyses for TRAIL-R1 and TRAIL-R2. Blots are representative and (E,F) show densitometric quantification normalized to loading control and the respective level of untreated cells cultured under pHe 7.4 conditions. Data are shown as mean with S.E.M error bars, of at least three independent experiments per cell line. **<0.01, ***<0.001, and ****<0.0001: significant difference between untreated and treated cells using two-way ANOVA with Sidak’s multiple-comparison test or between pHe conditions using two-way ANOVA with Tukey’s multiple-comparison test. Image collected and cropped by CiteAb from the following open publication (//pubmed.ncbi.nlm.nih.gov/35281089), licensed under a CC-BY license. Not internally tested by R&D Systems.

• Reactivity: Hu
• Applications: Flow
• Clone: 71908
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Allophycocyanin

TRAILR2/TNFRSF10B Antibody (71908) [Allophycocyanin] Summary

• Immunogen: Mouse myeloma cell line NS0-derived recombinant human TRAIL R2/TNFRSF10B
  Ile56-Glu182
  Accession # O14763
• Specificity: Detects human TRAIL R2 in direct ELISAs and Western blots. In direct ELISAs, does not cross-react with recombinant human (rh) TRAIL R1, rhTRAIL R3, rhTRAIL R4, or recombinant mouse TRAIL R2.
• Source: N/A
• Isotype: IgG2b
• Clonality: Monoclonal
• Host: Mouse
• Gene: TNFRSF10B
• Purity Statement: Protein A or G purified from hybridoma culture supernatant

Applications/Dilutions

• Dilutions:
  • Flow Cytometry 10 uL/10^6 cells

Packaging, Storage & Formulations

• Storage: Protect from light. Do not freeze.
  • 12 months from date of receipt, 2 to 8 °C as supplied.
• Buffer: Supplied in a saline solution containing BSA and Sodium Azide.
• Preservative: Sodium Azide

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for TRAILR2/TNFRSF10B Antibody (71908) [Allophycocyanin]

• CD262 antigen
• CD262
• death domain containing receptor for TRAIL/Apo-2L
• Death receptor 5
• DR5
• DR5TRICK2B
• Fas-like protein
• KILLER/DR5
• KILLERapoptosis inducing protein TRICK2A/2B
• TNF-related apoptosis-inducing ligand receptor 2
• TNFRSF10B
• TRAIL R2
• TRAIL receptor 2
• TRAILR2
• TRAIL-R2apoptosis inducing receptor TRAIL-R2
• TRAILR2cytotoxic TRAIL receptor-2
• TRICK2
• TRICK2A
• TRICKB
• tumor necrosis factor receptor superfamily member 10B
• tumor necrosis factor receptor superfamily, member 10b
• tumor necrosis factor receptor-like protein ZTNFR9
• ZTNFR9

Background

Human TRAIL R2, also known as DR5 and TRICK 2, is a type 1, TNF R family, membrane protein which is a receptor for TRAIL (APO2 ligand). In the new TNF superfamily nomenclature, TRAIL R2 is referred to as TNFRSF10B. TRAIL R2 cDNA encodes a 440 amino acid residue precursor protein containing extracellular cysteine-rich domains, a transmembrane domain and a cytoplasmic death domain. Among TNF receptor family proteins, TRAIL R2 is most closely related to TRAIL R1/DR4, sharing 55% amino acid sequence identity. Binding of trimeric TRAIL to TRAIL R2 induces apoptosis. The induction of apoptosis likely requires oligomerization of the receptor. The human TRAIL R2/Fc chimera neutralizes the ability of TRAIL to induce apoptosis. Besides TRAIL R2, an additional TRAIL R1/DR4, which tranduces apoptosis signaling, and two TRAIL decoy receptors, which antagonize TRAIL-induced apoptosis, have been reported.

1. Chaudhary, P.M. et al. (1997) Immunity 7:821.
2. Walczak, H. et al. (1997) EMBO J. 16:5386.
3. Golstein, P. (1997) Curr. Biol. 7:R750.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for TRAILR2/TNFRSF10B Antibody (FAB6311A)(5)

Publications using FAB6311A

• Sofie Hagelund, Anna Trauzold Impact of Extracellular pH on Apoptotic and Non-Apoptotic TRAIL-Induced Signaling in Pancreatic Ductal Adenocarcinoma Cells Frontiers in Cell and Developmental Biology 2022-02-24 [PMID: 35281089]
• KS Fakiruddin, MN Lim, N Nordin, R Rosli, Z Zakaria, S Abdullah Targeting of CD133+ Cancer Stem Cells by Mesenchymal Stem Cell Expressing TRAIL Reveals a Prospective Role of Apoptotic Gene Regulation in Non-Small Cell Lung Cancer Cancers (Basel), 2019-08-28;11(9):. 8/28/2019 [PMID: 31466290]
• U Mert, A Adawy, E Scharff, P Teichmann, A Willms, V Haselmann, C Colmorgen, J Lemke, S von Karste, J Fritsch, A Trauzold TRAIL Induces Nuclear Translocation and Chromatin Localization of TRAIL Death Receptors Cancers (Basel), 2019-08-14;11(8):. 2019-08-14 [PMID: 31416165] (Flow Cytometry, Human)
• A Willms, H Schittek, S Rahn, J Sosna, U Mert, D Adam, A Trauzold Impact of p53 status on TRAIL-mediated apoptotic and non-apoptotic signaling in cancer cells PLoS ONE, 2019-04-04;14(4):e0214847. 2019-04-04 [PMID: 30947287] (Flow Cytometry, Human)
• Meng X, Koh B, Zhang J, Flatten K, Schneider P, Billadeau D, Hess A, Smith B, Karp J, Kaufmann S Poly(ADP-ribose) polymerase inhibitors sensitize cancer cells to death receptor-mediated apoptosis by enhancing death receptor expression. J Biol Chem, 2014-07-25;289(30):20543-58. 2014-07-25 [PMID: 24895135] (Flow Cytometry, Human)

Bioinformatics

• Gene Symbol: TNFRSF10B
• Uniprot:
  • O14763()