TRAILR1/TNFRSF10A Antibody (32A242) - BSA Free

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Western Blot: TRAIL R1/TNFRSF10A Antibody (32A242) [NB100-56528] - Analysis using Azide/BSA FREE version of NB100-56528. Analysis of 20 ug of total cell lysate from HeLa cells with DR4 antibody at 2 ug/ml. Goat ...read more
Western Blot: TRAIL R1/TNFRSF10A Antibody (32A242) [NB100-56528] - Analysis of 20 ug of total cell lysate from HeLa cells with DR4 antibody at 2 ug/ml. Goat anti-mouse Ig HRP secondary antibody and PicoTect ECL ...read more

Product Details

Summary
Reactivity Hu, Mu, RtSpecies Glossary
Applications WB, IB, ICC/IF
Clone
32A242
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Format
BSA Free
Concentration
1.0 mg/ml

Order Details

TRAILR1/TNFRSF10A Antibody (32A242) - BSA Free Summary

Immunogen
This monoclonal antibody was raised against a peptide corresponding to amino acid 2 to 21 of human DR4.
Specificity
In HeLa, a 57 kDa band should be observed.
Isotype
IgG1
Clonality
Monoclonal
Host
Mouse
Gene
TNFRSF10A
Purity
Protein G purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Immunoblotting
  • Immunocytochemistry/Immunofluorescence
  • Western Blot 1 - 3 ug/ml
Application Notes
Use in Immunocytochemistry/immunofluorescence reported in scientific literature (PMID 23988408). Use in immunoblotting reported in scientific literature (PMID: 31534206).
Control
HeLa Whole Cell Lysate
Publications
Read Publications using
NB100-56528 in the following applications:

  • IB
    1 publication
  • 1 publication
  • WB
    3 publications

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS
Preservative
0.05% Sodium Azide
Concentration
1.0 mg/ml
Purity
Protein G purified

Alternate Names for TRAILR1/TNFRSF10A Antibody (32A242) - BSA Free

  • APO2
  • CD261 antigen
  • CD261
  • cytotoxic TRAIL receptor
  • Death receptor 4
  • DR4 TRAIL receptor 1
  • DR4
  • TNF-related apoptosis inducing ligand receptor 1
  • TNF-related apoptosis-inducing ligand receptor 1
  • TNFRSF10A
  • TRAIL R1
  • TRAILR1
  • TRAIL-R1
  • TRAILR-1
  • TRAILR1MGC9365
  • tumor necrosis factor receptor superfamily member 10A
  • tumor necrosis factor receptor superfamily, member 10a

Background

Cells undergo programmed cell death (Apoptosis) in response to various stimuli. Apoptosis is essential for morphogenesis, tissue homeostasis, and host defense. Apoptosis is induced by certain cytokines including TNF and Fas ligand in the TNF family through their death domain containing receptors, TNFR1 and Fas. A novel death domain containing receptor was recently identified and designated DR4 (for death receptor 4). The ligand for this novel death receptor has been identified and termed TRAIL, which is a new member in the TNF family. DR4 is also called TRAIL receptor-1 (TRAIL-R1). DR4 is expressed in most human tissues including spleen, peripheral blood leukocytes, small intestine and thymus. Like TNFR1, Fas and DR3, DR4 mediates apoptosis and NF-kB activation in many tissues and cells.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Supplier Logo

Publications for TRAILR1/TNFRSF10A Antibody (NB100-56528)(8)

We have publications tested in 1 confirmed species: Human.

We have publications tested in 3 applications: IB, ICC/IF, WB.


Filter By Application
IB
(1)
ICC/IF
(1)
WB
(3)
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Filter By Species
Human
(2)
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Showing Publications 1 - 8 of 8.
Publications using NB100-56528 Applications Species
Lee SR, Quan KT, Byun HS et al. Accelerated degradation of cFLIPL and sensitization of the TRAIL DISC-mediated apoptotic cascade by pinoresinol, a lignan isolated from Rubia philippinensis Sci Rep Sep 18 2019 [PMID: 31534206] (IB, Human) IB Human
Wen Q, Wang W, Luo J et al. CGP57380 enhances efficacy of RAD001 in non-small cell lung cancer through abrogating mTOR inhibition-induced phosphorylation of eIF4E and activating mitochondrial apoptotic pathway. Oncotarget. Mar 30 2016 [PMID: 27050281] (WB, Human) WB Human
Di Xu, Zhang Guofeng, Zhang Yaqin et al. Accumulation of autophagosomes in breast cancer cells induces TRAIL resistance through downregulation of surface expression of death receptors 4 and 5. Oncotarget. 2013 Sep [PMID: 23988408] (ICC/IF) ICC/IF
Brooks AD, Jacobsen KM, Li W et al. Bortezomib sensitizes human renal cell carcinomas to TRAIL apoptosis through increased activation of caspase-8 in the death-inducing signaling complex. Mol Cancer Res. 2010 May [PMID: 20442297]
Laguinge LM, Samara RN, Wang W et al. DR5 receptor mediates anoikis in human colorectal carcinoma cell lines. Cancer Res. 2008 Feb 1 [PMID: 18245494]
Xu J, Zhou JY, Wu GS. Tumor necrosis factor-related apoptosis-inducing ligand is required for tumor necrosis factor alpha-mediated sensitization of human breast cancer cells to chemotherapy. Cancer Res. 2006 Oct 15 [PMID: 17047073]
Cisternas P, Moreno RD. Comparative analysis of apoptotic pathways in rat, mouse, and hamster spermatozoa. Mol Reprod Dev. 2006 Oct [PMID: 16868928] (WB)

Details:
DR4/TRAILR1 (NT) (IMG-275). WB: Mouse, rat, and hamster spermatozoa, Fig 3A. Note: The DR4/TRAIL1 antibody was detected in mouse and rat spermatozoa but not in hamster, Fig 3A.
WB
Jin F, Liu X, Zhou Z et al. Activation of nuclear factor-kappaB contributes to induction of death receptors and apoptosis by the synthetic retinoid CD437 in DU145 human prostate cancer cells. Cancer Res. 2005 Jul 15 [PMID: 16024638] (WB)

Details:
WB: 1. Caspase-3 mAb, clone 31A1067 (IMG-144A): Fig 3C, DU145 prostate adenocarcinoma cells.
WB

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Product General Protocols

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Video Protocols

WB Video Protocol
ICC/IF Video Protocol

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Control Lysate(s)

Secondary Antibodies

 

Isotype Controls

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Bioinformatics

Gene Symbol TNFRSF10A
Entrez
Uniprot