TRAILR1/TNFRSF10A Antibody (32A242) - Azide and BSA Free

Western Blot: TRAILR1/TNFRSF10A Antibody (32A242) - Azide Free [NBP2-27415] - Analysis of 20 ug of total cell lysate from HeLa cells with DR4 antibody at 2 ug/ml. Goat anti-mouse Ig HRP secondary antibody and PicoTect ECL substrate solution were used for this test.

• Reactivity: Hu, Mu, Rt
• Applications: WB
• Clone: 32A242
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Format: Azide and BSA Free

TRAILR1/TNFRSF10A Antibody (32A242) - Azide and BSA Free Summary

• Description: Novus Biologicals Mouse TRAILR1/TNFRSF10A Antibody (32A242) - Azide and BSA Free (NB100-56528) is a monoclonal antibody validated for use in WB. Anti-TRAILR1/TNFRSF10A Antibody: Cited in 4 publications. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: This monoclonal antibody was raised against a peptide corresponding to amino acid 2 to 21 of human DR4.
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Mouse
• Gene: TNFRSF10A
• Purity: Protein G purified

Applications/Dilutions

• Dilutions:
  • Western Blot 1-3ug/ml
• Application Notes: In HeLa, a 57 kDa band should be observed.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: Sterile - filtered PBS
• Preservative: No Preservative
• Purity: Protein G purified

Alternate Names for TRAILR1/TNFRSF10A Antibody (32A242) - Azide and BSA Free

• APO2
• CD261 antigen
• CD261
• cytotoxic TRAIL receptor
• Death receptor 4
• DR4 TRAIL receptor 1
• DR4
• TNF-related apoptosis inducing ligand receptor 1
• TNF-related apoptosis-inducing ligand receptor 1
• TNFRSF10A
• TRAIL R1
• TRAILR1
• TRAIL-R1
• TRAILR-1
• TRAILR1MGC9365
• tumor necrosis factor receptor superfamily member 10A
• tumor necrosis factor receptor superfamily, member 10a

Background

Cells undergo programmed cell death in response to various stimuli. Apoptosis is essential for morphogenesis, tissue homeostasis, and host defense (5). It is induced by certain cytokines including TNF and Fas ligand in the TNF family through their death domain containing receptors, TNFR1 and Fas. A death domain containing receptor was identified and designated DR4 (4). The ligand for this death receptor has been identified and termed TRAIL (2,3), which is a new member in the TNF family. DR4 is also called TRAIL receptor-1 (TRAILR1) (1). DR4 is expressed in most human tissues including spleen, peripheral blood leukocytes, small intestine and thymus. Like TNFR1, Fas and DR3, DR4 mediates apoptosis and NF-kB activation in many tissues and cells.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for TRAILR1/TNFRSF10A Antibody (NBP2-27415)(4)

Publications using NBP2-27415

• Brooks AD, Jacobsen KM, Li W et al. Bortezomib sensitizes human renal cell carcinomas to TRAIL apoptosis through increased activation of caspase-8 in the death-inducing signaling complex. Mol Cancer Res. 2010-05-01 [PMID: 20442297] (WB)
• Laguinge LM, Samara RN, Wang W et al. DR5 receptor mediates anoikis in human colorectal carcinoma cell lines. Cancer Res. 2008-02-01 [PMID: 18245494] (WB, Human)
  
  Details:
  WB [human colerectal cancer (CRC) cells], Fig. 4A.
• Cisternas P, Moreno RD. Comparative analysis of apoptotic pathways in rat, mouse, and hamster spermatozoa. Mol Reprod Dev. 2006-10-01 [PMID: 16868928]
  
  Details:
  DR4/TRAILR1 (NT) (IMG-275). WB: Mouse, rat, and hamster spermatozoa, Fig 3A. Note: The DR4/TRAIL1 antibody was detected in mouse and rat spermatozoa but not in hamster, Fig 3A.
• Jin F, Liu X, Zhou Z et al. Activation of nuclear factor-kappaB contributes to induction of death receptors and apoptosis by the synthetic retinoid CD437 in DU145 human prostate cancer cells. Cancer Res. 2005-07-15 [PMID: 16024638] (WB)
  
  Details:
  IMGENEX antibodies cited for WB: 1. Caspase-3 mAb, clone 31A1067 (IMG-144A): Fig 3C, DU145 prostate adenocarcinoma cells. 2. DcR1 pAb (IMG-245-1/IMG-245-2): Fig 6C, DcR1 overexpressing DU145 cells. Note: The specificity of the DcR1 pAb was validated in Dc

Bioinformatics

• Gene Symbol: TNFRSF10A