TRAF-6 Inhibitor Peptide Set

Product Discontinued

TRAF-6 Inhibitor Peptide Set Summary

• Immunogen: Functions as a TRAF6 decoy by binding to the T6DP motif of RANK, thereby preventing binding of RANK to TRAF6.
• Specificity: The TRAF6 inhibitory peptide also contains a protein transduction (PTD) sequence (DRQIKIWFQNRRMKWKK) derived from antennapedia which renders the peptide cell permeable. The control peptide consists of only the PTD sequence.
• Preparation Method: Preparation of 5 mM Stock Solutions
  PBS* is added directly to the vials to prepare the stock solutions. Note: Bring the solution to room temperature and quick spin the tubes before opening the caps.
  
  TRAF6 Inhibitor Peptide: 1 mg of DRQIKIFQNRRMKWKKRKIPTEDEY
  Add 57.7 ul of PBS* to the vial to make a 5 mM stock solution. Mix by vortexing. Aliquot and store at -20C or -80C. Avoid repeated freeze thawing.
  
  Control Peptide: 1 mg of DRQIKIWFQNRRMKWKK
  Add 84.8 ul PBS* to the vial. Mix by vortexing. Aliquot and store at 20C or -80C. Avoid repeated freeze thawing.
  
  
  *Recipe for 1X PBS:
  1. Dissolve the following in 800ml distilled H2O.
  - 8g of NaCl
  - 0.2g of KCl
  - 1.44g of Na2HPO4
  - 0.24g of KH2PO4
  2. Adjust pH to 7.5 with HCl.
  3. Adjust volume to 1L with additional distilled H2O.
  4. Sterilize by autoclaving
• Content: TRAF6 Inhibitor peptide: 2 x 1 mg (lyophilized) DRQIKIWFQNRRMKWKKRKIPTEDEY (TRAF6 binding sequence: RKIPTEDEY). Molecular weight: 3494.
• Gene: TRAF6

Applications/Dilutions

• Dilutions:
  • Block/Neutralize
• Application Notes: Inhibition of RANKL mediated osteoclast differentiation. Researchers can study the effect of TRAF6 inhibitor peptide using a variety of methods, such as EMSA, NF-kB/p65 ELISA,IkBa phosphorylation ELISA, osteoclast differentiation assay. The Osteoclast formation assay protocol is a guideline which may need to be optimized for different cell types. Use in blocking/neutralizing reported in scientific literature (PMID 27624059).

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: Lyophilized white powder.
• Concentration: LYOPH
• Reconstitution Instructions: Please contact technical support for detailed reconstitution instructions.

Alternate Names for TRAF-6 Inhibitor Peptide Set

• E3 ubiquitin-protein ligase TRAF6
• EC 6.3.2.-
• Interleukin-1 signal transducer
• RING finger protein 85
• RNF85MGC:3310
• TNF receptor-associated factor 6
• TRAF6
• TRAF-6

Background

TRAF6 is an adapter protein that links RANK with downstream signaling molecules, and plays a key role in RANKL mediated osteoclast differentiation. RANK contains a novel TRAF6-binding motif (T6DP) through which it binds to TRAF6 upon activation by RANKL. The TRAF6 inhibitory peptide contains a sequence from the T6DP motif of mouse RANK. The peptide binds to RANK, blocking TRAF6 and RANK interaction and thereby inhibiting osteoclast differentiation. The TRAF6 inhibitory peptide also contains a protein transduction (PTD) sequence (DRQIKIWFQNRRMKWKK) derived from antennapedia which renders the peptide cell permeable.The control peptide consists of only the PTD sequence

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Inhibitors are guaranteed for 1 year from date of receipt.

Publications for TRAF-6 Inhibitor (NBP2-31223)(2)

Publications using NBP2-31223

• He LX, Tong X, Zeng J et al. Paeonol Suppresses Neuroinflammatory Responses in LPS-Activated Microglia Cells Inflammation 2016-09-13 [PMID: 27624059] (B/N, Mouse)
• Wu T, Mester T, Gupta S et al. TSH and CD40L Stimulate Interleukin-12 Expression in Fibrocytes: Implications for pathogenesis of Thyroid Associated Ophthalmopathy Thyroid 2016-09-09 [PMID: 27612658] (Control, B/N, Human)

Bioinformatics

• Gene Symbol: TRAF6