TRAF-4 Antibody (433709) [Alexa Fluor® 647]

• Reactivity: Hu
• Applications: WB
• Clone: 433709
• Clonality: Monoclonal
• Host: Rat
• Conjugate: Alexa Fluor 647

TRAF-4 Antibody (433709) [Alexa Fluor® 647] Summary

• Immunogen: E. coli-derived recombinant human TRAF-4
  Met1-Ser470
  Accession # Q9BUZ4
• Specificity: Detects endogenous human TRAF-4. In Western blots, this antibody does not cross-react with rhTRAF-1, -2, -3, -5, or -6 in Western blots.
• Isotype: IgG2a
• Clonality: Monoclonal
• Host: Rat
• Purity Statement: Protein A or G purified

Applications/Dilutions

• Dilutions:
  • Western Blot

Packaging, Storage & Formulations

• Storage: Protect from light. Do not freeze. 12 months from date of receipt, 2 to 8 degreesC as supplied
• Buffer: Supplied 0.2mg/ml in 1X PBS with RDF1 and 0.09% Sodium Azide

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for TRAF-4 Antibody (433709) [Alexa Fluor® 647]

• CART1
• CART1RING finger protein 83
• Cysteine-rich domain associated with RING and Traf domains protein 1
• Metastatic lymph node gene 62 protein
• MLN 62
• MLN62
• MLN62cysteine-rich domain associated with ring and TRAF domain
• RNF83malignant 62
• TNF receptor-associated factor 4
• TRAF4
• TRAF-4
• tumor necrosis receptor-associated factor 4A

Background

Tumor Necrosis Factor (TNF) Receptor-Associated Factors (TRAFs) are a family of adaptor proteins that interact with a wide range of cell surface receptors and participate in the regulation of cell survival, proliferation, differentiation, and stress response. TRAFs were identified by their ability to form complexes with TNF receptor superfamily members but more recently are reported to also bind to Toll/IL-1 receptor family members and mediate cellular signaling. Seven members of the TRAF family have been identified. All TRAF proteins have a homologous C-terminal TRAF domain that can bind the cytoplasmic domain of receptors as well as other TRAFs. TRAFs 2-6 have N-terminal RING and zinc finger domains that are involved in signaling downstream events. TRAF-4, also known as CART1 and MLN62, is a 470 amino acid, 53 kDa protein. A second isoform has been identified and lacks amino acids 157-428. TRAF-4 is unique member of the TRAF family that localizes to the nucleus and does not regulate signaling through cell surface receptors. TRAF-4 is highly expressed in human breast carcinomas and during embryogenisis.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Uniprot:
  • Q9BUZ4()