TRA-1-81 Antibody (TRA-1-81) [DyLight 650]

Product Discontinued

TRA-1-81 Antibody (TRA-1-81) [DyLight 650] Summary

• Immunogen: Human embryonal carcinoma cell line 2102Ep.
• Localization: Cell Membrane
• Marker: Embryonic Stem Cell Marker, Tumor Vessel Marker
• Isotype: IgM
• Clonality: Monoclonal
• Host: Mouse
• Purity: IgM purified

Applications/Dilutions

• Dilutions:
  • Flow Cytometry
  • Immunocytochemistry/ Immunofluorescence
  • Immunohistochemistry
  • Immunohistochemistry-Frozen
  • Immunohistochemistry-Paraffin
  • Immunoprecipitation
  • Western Blot
• Application Notes: This TRA-1-81 antibody is useful for Immunofluorescence/Immunocytochemistry, Immunohistochemistry-Frozen, Immunohistochemistry-Paraffin, Immunoprecipitation and Western Blot. Flow Cytometry was reported in scientific literature.

Reactivity Notes

Human. This antibody recognizes the TRA-1-81 antigen that is expressed upon the surface of human teratocarcinoma stem cells (EC), human embryonic germ cells (EG) and human embryonic stem cells (ES). No immunoreactivity is seen with murine EC, EG or ES cells. This antibody recognizes antigens that are associated with a pericellular matrix proteoglycan.

Packaging, Storage & Formulations

• Storage: Store at 4C in the dark.
• Buffer: 50mM Sodium Borate
• Preservative: 0.05% Sodium Azide
• Purity: IgM purified

Notes

Dylight (R) is a trademark of Thermo Fisher Scientific Inc. and its subsidiaries.

Alternate Names for TRA-1-81 Antibody (TRA-1-81) [DyLight 650]

• GCTM-2 antigen
• GP135
• gp200
• PC
• PCLP
• PCLP-1
• PDX
• Podocalyxin
• Podocalyxin-like protein 1
• PODXL1
• TRA181
• TRA-1-81

Background

TRA-1-81 is a cell surface protein involved in cell differentiation. Cell surface antigens are powerful tools for characterizing the function and expression of developmentally regulated cells during both embryogenesis and oncogenesis.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for TRA-1-81 Antibody (NB100-1833C)(2)

Publications using NB100-1833C

• Rossbach B, Hildebrand L, El-Ahmad L. Generation of a human induced pluripotent stem cell line from urinary cells of a healthy donor using an integration free vector. Stem Cell Res 2016-06-28 [PMID: 27345993]
• Hildebrand L, Seemann P, Kurtz A et al. Selective cell targeting and lineage tracing of human induced pluripotent stem cells using recombinant avian retroviruses Cell. Mol Life Sci 2015-06-25 [PMID: 26109426] (ICC/IF, Human)
  
  Details:
  This publication used the DL650 conjugated form of this antibody (NB100-1833C)