TRA-1-81 Antibody (TRA-1-81) [Alexa Fluor® 488]

BG01V human embryonic stem cells were stained with Mouse Anti-Human TRA-1-81 Alexa Fluor® 488-conjugated Monoclonal Antibody (Catalog # FAB8495G, filled histogram) or isotype control antibody (Catalog # IC015G, open histogram). View our protocol for Staining Membrane-associated Proteins.

TRA-1-81 was detected in immersion fixed BG01V human embryonic stem cells using Mouse Anti-Human TRA-1-81 Alexa Fluor® 488‑conjugated Monoclonal Antibody (green; Catalog # FAB8495G) at 10 µg/mL for 3 hours at room temperature. Cells were counterstained with DAPI (blue). Specific staining was localized to cell membranes. View our protocol for Fluorescent ICC Staining of Stem Cells on Coverslips.

Product Discontinued

TRA-1-81 Antibody (TRA-1-81) [Alexa Fluor® 488] Summary

• Immunogen: Human embryonal carcinoma cell line 2102Ep
• Specificity: Detects human TRA-1-81 in flow cytometry.
• Source: N/A
• Isotype: IgM
• Clonality: Monoclonal
• Host: Mouse
• Gene: PODXL
• Purity Statement: Protein A or G purified from hybridoma culture supernatant

Applications/Dilutions

• Dilutions:
  • Flow Cytometry 5 uL/10^6 cells
  • Immunocytochemistry 8-25 ug/mL

Packaging, Storage & Formulations

• Storage: Protect from light. Do not freeze.
  • 12 months from date of receipt, 2 to 8 °C as supplied.
• Buffer: Supplied in a saline solution containing BSA and Sodium Azide.
• Preservative: Sodium Azide

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for TRA-1-81 Antibody (TRA-1-81) [Alexa Fluor® 488]

• GCTM-2 antigen
• GP135
• gp200
• PC
• PCLP
• PCLP-1
• PDX
• Podocalyxin
• Podocalyxin-like protein 1
• PODXL1
• TRA181
• TRA-1-81

Background

The TRA-1-81 antibody reacts with a high-molecular-mass carbohydrate epitope on the surface of human embryonal carcinoma (EC), embryonic germ (EG), embryonic stem (ES), and induced pluripotent stem (iPS) cells.  TRA-1-81 is an epitope expressed on podocalyxin, also known as Podocalyxin-Like Protein-1 (PCLP1 or PODXL), a type I transmembrane glycoprotein.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: PODXL