TRA-1-60® Antibody (TRA-1-60) [NL557]

The stem cell marker TRA-1-60(R) was visualized in live BG01V human embryonic stem cells using a GloLIVE NL557-conjugated Mouse Anti-Human TRA-1-60(R) Monoclonal Antibody (Catalog # NLLC4770R; red). The nuclei were counterstained with Hoechst 33342 (blue). Positive staining for TRA-1-60(R) in human stem cells is an indicator of pluripotency.

• Reactivity: Hu
• Applications: ICC/IF
• Clone: TRA-1-60
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: NL557

TRA-1-60® Antibody (TRA-1-60) [NL557] Summary

• Additional Information: 50X Concentration, Azide Free
• Specificity: Detects human TRA‑1‑60(R).
• Isotype: IgM
• Clonality: Monoclonal
• Host: Mouse
• Gene: PODXL

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry

Packaging, Storage & Formulations

• Storage: Store the unopened product at 2 - 8° C. Do not use past expiration date. Protect from light.
• Buffer: Supplied in a saline solution containing BSA and Sodium Azide.
• Preservative: Sodium Azide

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for TRA-1-60(R) Antibody (TRA-1-60) [NL557]

• GCTM-2 antigen
• GP135
• gp200
• PC
• PCLP
• PCLP-1
• PDX
• Podocalyxin
• Podocalyxin-like protein 1
• PODXL1
• TRA160(R)
• TRA-1-60(R)

Background

TRA-1-60 is a monoclonal antibody raised against a cell surface antigen of human embryonal carcinoma (EC) cells (1). The TRA-1-60 epitope is also found on human embryonic stem (ES) cells and primordial germ cells, and TRA-1-60 serves as a serum marker in patients with germ cell tumors (2‑4). Investigation into the identity of the TRA-1-60 epitope demonstrated that it is a carbohydrate carried by a cell surface, sialylated, keratan sulfate proteoglycan (5). Subsequent evidence implicated podocalyxin as a carrier for the TRA-1-60 epitope (6).

1. Andrews, P. et al. (1984) Hybridoma 3:347.
2. Thomson, J. et al. (1998) Science 282:1145.
3. Giwercman, A. et al. (1993) Cancer 72:1308.
4. Marrink, J. et al. (1991) Int. J. Cancer 49:368.
5. Badcock, G. et al. (1999) Cancer Res. 59:4715.
6. Schopperle, W. and W. DeWolf (2007) Stem Cells 25:723.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for TRA-1-60(R) Antibody (NLLC4770R)(2)

Publications using NLLC4770R

• T Okumura, Y Horie, CY Lai, HT Lin, H Shoda, B Natsumoto, K Fujio, E Kumaki, T Okano, S Ono, K Tanita, T Morio, H Kanegane, H Hasegawa, F Mizoguchi, K Kawahata, H Kohsaka, H Moritake, H Nunoi, H Waki, SI Tamaru, T Sasako, T Yamauchi, T Kadowaki, H Tanaka, S Kitanaka, K Nishimura, M Ohtaka, M Nakanishi, M Otsu Robust and highly efficient hiPSC generation from patient non-mobilized peripheral blood-derived CD34+ cells using the auto-erasable Sendai virus vector Stem Cell Res Ther, 2019-06-24;10(1):185. 2019-06-24 [PMID: 31234949] (ICC, Human)
• S Thekkepara, S Sriram, S Subramania, S Cheng, WK Ong, S Rozen, NHA Kasim, S Sugii Amenable epigenetic traits of dental pulp stem cells underlie high capability of xeno-free episomal reprogramming Stem Cell Res Ther, 2018-03-20;9(1):68. 2018-03-20 [PMID: 29559008] (Human)

Bioinformatics

• Gene Symbol: PODXL