TRA-1-60® Antibody (TRA-1-60) [Alexa Fluor® 488]

Product Discontinued

TRA-1-60® Antibody (TRA-1-60) [Alexa Fluor® 488] Summary

• Immunogen: Human embryonal carcinoma cell line 2102Ep.
• Localization: Cell Membrane
• Marker: Embryonic Stem Cell Marker
• Specificity: Specific for the neuraminidase resistant form of the TRA-1-60 antigen that is expressed upon the surface of human teratocarcinoma stem cells (EC), human embryonic germ cells (EG) and human embryonic stem cells (ES). No immunoreactivity is seen with murine EC, EG or ES cells. TRA-1-60 reacts with a sialidase-sensitive epitope.
• Isotype: IgM
• Clonality: Monoclonal
• Host: Mouse
• Purity: IgM purified

Applications/Dilutions

• Dilutions:
  • ELISA
  • Flow Cytometry
  • Immunocytochemistry/ Immunofluorescence
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin
  • Immunoprecipitation
  • Western Blot
• Application Notes: This TRA-1-60 (R) antibody is useful for Flow Cytometry, Immunocytochemistry/Immunofluorescence, Immunohistochemistry, Immunoprecipitation and Western blot.

Reactivity Notes

Human. No immunoreactivity is seen with murine EC, EG or ES cells.

Packaging, Storage & Formulations

• Storage: Store at 4C in the dark.
• Buffer: 50mM Sodium Borate
• Preservative: 0.05% Sodium Azide
• Purity: IgM purified

Notes

Alexa Fluor (R) products are provided under an intellectual property license from Life Technologies Corporation. The purchase of this product conveys to the buyer the non-transferable right to use the purchased product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). The sale of this product is expressly conditioned on the buyer not using the product or its components, or any materials made using the product or its components, in any activity to generate revenue, which may include, but is not limited to use of the product or its components: (i) in manufacturing; (ii) to provide a service, information, or data in return for payment; (iii) for therapeutic, diagnostic or prophylactic purposes; or (iv) for resale, regardless of whether they are resold for use in research. For information on purchasing a license to this product for purposes other than as described above, contact Life Technologies Corporation, 5791 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@lifetech.com. This conjugate is made on demand. Actual recovery may vary from the stated volume of this product. The volume will be greater than or equal to the unit size stated on the datasheet.

Alternate Names for TRA-1-60(R) Antibody (TRA-1-60) [Alexa Fluor® 488]

• GCTM-2 antigen
• GP135
• gp200
• PC
• PCLP
• PCLP-1
• PDX
• Podocalyxin
• Podocalyxin-like protein 1
• PODXL1
• TRA160(R)
• TRA-1-60(R)

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for TRA-1-60(R) Antibody (NB100-730AF488)(2)

Publications using NB100-730AF488

• Smullen M, Olson MN, Reichert JM et al. Reliable multiplex generation of pooled induced pluripotent stem cells Cell reports methods 2023-09-25 [PMID: 37751688] (FLOW, Human)
• Smullen M, Reichert J, Dawes P et al. Reliable Multiplex Generation of Pooled Induced Pluripotent Stem Cells for Genetic Testing SSRN Electronic Journal 2022-07-14 (FLOW, Human)