TR alpha/NR1A1/Thyroid Hormone Receptor alpha Antibody (JE40-49)

Western Blot: TR alpha/NR1A1/Thyroid Hormone Receptor alpha Antibody (JE40-49) [NBP3-33015] - Western blot analysis of TR alpha/NR1A1/Thyroid Hormone Receptor alpha on different lysates with Rabbit anti-TR alpha/NR1A1/Thyroid Hormone Receptor alpha antibody (NBP3-33015) at 1/1,000 dilution. Lane 1: A431 cell lysate Lane 2: SW480 cell lysate Lysates/proteins at 20 ug/Lane. Predicted band size: 55 kDa Observed band size: 55 kDa Exposure time: 3 minutes; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-33015) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:100,000 dilution was used for 1 hour at room temperature.

• Reactivity: Hu
• Applications: WB
• Clone: JE40-49
• Clonality: Monoclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Concentration: 1 mg/ml

TR alpha/NR1A1/Thyroid Hormone Receptor alpha Antibody (JE40-49) Summary

• Description: Novus Biologicals Rabbit TR alpha/NR1A1/Thyroid Hormone Receptor alpha Antibody (JE40-49) (NBP3-33015) is a recombinant monoclonal antibody validated for use in WB. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Additional Information: Recombinant Monoclonal Antibody
• Immunogen: Synthetic peptide within Human TR alpha/NR1A1/Thyroid Hormone Receptor alpha aa 416-465 / 490. (Uniprot: P10827)
• Localization: Nucleus; Cytoplasm, Nucleus.
• Source: HEK293
• Isotype: IgG
• Clonality: Monoclonal
• Host: Rabbit
• Gene: THRA
• Purity: Protein A purified

Applications/Dilutions

• Dilutions:
  • Western Blot 1:1000
• Theoretical MW: 55 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: 1*TBS (pH7.4), 0.05% BSA and 40% Glycerol
• Preservative: 0.05% Sodium Azide
• Concentration: 1 mg/ml
• Purity: Protein A purified

Alternate Names for TR alpha/NR1A1/Thyroid Hormone Receptor alpha Antibody (JE40-49)

• AR7
• c-ERBA-1
• c-erbA-alpha
• EAR7
• EAR-7
• EAR-7.1/EAR-7.2
• ERBA
• ERBA1thyroid hormone receptor, alpha (avian erythroblastic leukemia viral (v-erb-a)oncogene homolog)
• ERBA-related 7
• MGC000261
• MGC43240
• NR1A 1
• NR1A1
• NR1A1THRA3
• Nuclear receptor subfamily 1 group A member 1
• THRA
• THRA1ERB-T-1
• THRA2
• thyroid hormone receptor alpha
• thyroid hormone receptor, alpha (erythroblastic leukemia viral (v-erb-a)oncogene homolog, avian)
• TR alpha
• triiodothyronine receptor
• v-ErbA1
• V-erbA-related protein 7

Background

Thyroid Hormone Receptor alpha is encoded by this gene is a nuclear hormone receptor for triiodothyronine. It is one of the several receptors for thyroid hormone, and has been shown to mediate the biological activities of thyroid hormone. Knockout studies in mice suggest that the different receptors, while having certain extent of redundancy, may mediate different functions of thyroid hormone. Alternatively spliced transcript variants encoding distinct isoforms have been reported.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: THRA