TPT1/TCTP Antibody

Western blot shows lysates of Jurkat human acute T cell leukemia cell line and Nalm-6 human Pre-B acute lymphocytic leukemia cell line. PVDF membrane was probed with 1 µg/mL of Goat Anti-Human TPT1/TCTP Antigen Affinity-purified Polyclonal Antibody (Catalog # AF4648) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF019). A specific band was detected for TPT1/TCTP at approximately 22 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 8.

• Reactivity: Hu
• Applications: WB
• Clonality: Polyclonal
• Host: Goat
• Conjugate: Unconjugated
• Concentration: LYOPH

TPT1/TCTP Antibody Summary

• Immunogen: E. coli-derived recombinant human TPT1/TCTP
  Met1-Cys172
  Accession # P13693
• Specificity: Detects human TPT1/TCTP in direct ELISAs and Western blots. In direct ELISAs, approximately 50% cross-reactivity with recombinant mouse TPT1 is observed.
• Source: N/A
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Gene: TPT1
• Purity Statement: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • Western Blot 1 ug/mL

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Preservative: No Preservative
• Concentration: LYOPH
• Reconstitution Instructions: Reconstitute at 0.2 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for TPT1/TCTP Antibody

• FLJ27337
• Fortilin
• Histamine-releasing factor
• HRF
• p02
• p23
• TCTP
• TCTPHRFfortilin
• TPT1
• translationally-controlled tumor protein
• tumor protein, translationally-controlled 1

Background

TPT-1 (translationally controlled tumor protein 1; also TCTP, HRF and fortilin) is a 22-23 kDa member of the TCTP family of calcium-binding proteins. It is widely expressed (but not in kidney), and apparently serves as a transcriptional activator (Oct4), calcium transporter, histamine inducer and antiapoptotic, caspase-3 inhibitor. Human TPT-1 is 172 amino acids (aa) in length. It contains little in the way of structural motifs. There are multiple potential splice variants. There is an alternate start site at Met35 that may be accompanied by a deletion of aa 99-172. In addition, there may be either a 25 aa or 16 aa C-terminal extension after Cys172. Over aa 1-172, human TPT-1 is 96% aa identical to mouse TPT-1.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: TPT1
• Uniprot:
  • P13693()