TPT1/TCTP Antibody (2C4)

Western Blot: TPT1/TCTP Antibody (2C4) [H00007178-M03] - Analysis of TPT1 expression in MCF-7 (Cat # L046V1).

Western Blot: TPT1/TCTP Antibody (2C4) [H00007178-M03] - Analysis of TPT1 expression in PC-12 (Cat # L012V1).

Western Blot: TPT1/TCTP Antibody (2C4) [H00007178-M03] - Analysis of TPT1 expression in Raw 264.7 (Cat # L024V1).

Western Blot: TPT1/TCTP Antibody (2C4) [H00007178-M03] - Analysis of TPT1 expression in NIH/3T3 (Cat # L018V1).

ELISA: TPT1/TCTP Antibody (2C4) [H00007178-M03] - Detection limit for recombinant GST tagged TPT1 is approximately 0.3ng/ml as a capture antibody.

• Reactivity: Hu, Mu, Rt
• Applications: WB, ELISA
• Clone: 2C4
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated

TPT1/TCTP Antibody (2C4) Summary

• Description: Quality control test: Antibody Reactive Against Recombinant Protein.
• Immunogen: TPT1 (AAH22436, 35 a.a. ~ 138 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. GVDIVMNHHLQETSFTKEAYKKYIKDYMKSIKGKLEEQRPERVKPFMTGAAEQIKHILANFKNYQFFIGENMNPDGMVALLDYREDGVTPYMIFFKDGLEMEKC
• Specificity: tumor protein, translationally-controlled 1
• Isotype: IgG2a Kappa
• Clonality: Monoclonal
• Host: Mouse
• Gene: TPT1
• Purity: IgG purified

Applications/Dilutions

• Dilutions:
  • ELISA
  • Western Blot
• Application Notes: Antibody reactivity against cell lysate and recombinant protein for WB. It has also been used for ELISA.

Reactivity Notes

Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Additional Mouse on Mouse blocking steps may be required for IHC and ICC experiments. Please contact Technical Support for more information.

Packaging, Storage & Formulations

• Storage: Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
• Buffer: In 1x PBS, pH 7.4
• Preservative: No Preservative
• Purity: IgG purified

Notes

This product is produced by and distributed for Abnova, a company based in Taiwan.

Alternate Names for TPT1/TCTP Antibody (2C4)

• FLJ27337
• Fortilin
• Histamine-releasing factor
• HRF
• p02
• p23
• TCTP
• TCTPHRFfortilin
• TPT1
• translationally-controlled tumor protein
• tumor protein, translationally-controlled 1

Background

Histamine is an inflammatory mediator that is ubiquitously expressed and has a broad range of pharmacologic effects. Specifically, it plays a role in the central nervous, gastrointestinal, respiratory and immune systems. Histamine release is mediated by the stimulation of mast cells and basophils. Histamine-releasing factor (HRF) is a cytokine-like molecule that causes the release of histamine, IL-4 and IL-13 from basophils as well as the secretion of IL-8 and a calcium response in eosinophils. HRF belongs to the translationally controlled tumor protein (TCTP) family and is detected as a protein of 21 kDa in mice and 23 kDa in human. It is expressed in several healthy and tumoral cells, including erythrocytes, hepatocytes, macrophages, platelets, keratinocytes, erythroleukemia cells, gliomas, melanomas, hepatoblastomas and lymphomas, and it is localized in the cytoplasm. HRF plays a pivotal role in allergic diseases, and due to its wide distribution in brain, is thought to be involved in neurodegenerative disorders, such as Alzheimer's disease and Down Syndrome.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for TPT1/TCTP Antibody (H00007178-M03)(3)

Publications using H00007178-M03

• Kazumi K, Yu K, Alain J et al. Histamine-Releasing Factor Is a Novel Alarmin Induced by House Dust Mite Allergen, Cytokines, and Cell Death. J Immunol. 2022-10-12 [PMID: 36223930]
• Nusair SD, Joukhan AN, Rashaid AB, Rababa'h AM et al. Methomyl induced effect on fortilin and S100A1 in serum and cardiac tissue: Potential biomarkers of toxicity. Hum Exp Toxicol. 2018-11-25 [PMID: 30472887]
• Sinthujaroen P, Wanachottrakul N, Pinkaew D et al. Elevation of serum fortilin levels is specific for apoptosis and signifies cell death in vivo. BBA Clinical. 2014-01-01 [PMID: 25558447]

Bioinformatics

• Gene Symbol: TPT1
• Entrez:
  • 7178(Human)
• OMIM:
  • 600763(Human)
• Uniprot:
  • AAH22436(Human)