TPK1 Antibody - BSA Free

Immunohistochemistry-Paraffin: TPK1 Antibody [NBP1-83151] - Staining of human tonsil shows strong cytoplasmic positivity in non-germinal center cells.

Immunohistochemistry-Paraffin: TPK1 Antibody [NBP1-83151] - Staining of human placenta shows strong cytoplasmic positivity in trophoblastic cells.

Immunohistochemistry-Paraffin: TPK1 Antibody [NBP1-83151] - Staining of human colon shows moderate to strong cytoplasmic positivity in glandular cells.

Immunohistochemistry: TPK1 Antibody [NBP1-83151] - Staining of human kidney shows weak to moderate cytoplasmic positivity in cells in tubules.

Immunohistochemistry-Paraffin: TPK1 Antibody [NBP1-83151] - Staining of human skin shows no positivity in squamous epithelial cells as expected.

• Reactivity: Hu
• Applications: IHC
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: BSA Free

TPK1 Antibody - BSA Free Summary

• Description: Novus Biologicals Rabbit TPK1 Antibody - BSA Free (NBP1-83151) is a polyclonal antibody validated for use in IHC. Anti-TPK1 Antibody: Cited in 1 publication. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: This antibody was developed against Recombinant Protein corresponding to amino acids: AFTPLEPLLSTGNLKYCLVILNQPLDNYFRHLWNKALLRACADGGANRLYDITEGERESFLPEFINGDFDSI
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: TPK1
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry 1:50 - 1:200
  • Immunohistochemistry-Paraffin 1:50 - 1:200
• Application Notes: For IHC-Paraffin, HIER pH 6 retrieval is recommended. IF fixation permeabilization: PFA/Triton X-100.
• Control Peptide:

  TPK1 Protein (NBP1-83151PEP)

Reactivity Notes

Immunogen displays the following percentage of sequence identity for non-tested species: Mouse (88%)

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS (pH 7.2) and 40% Glycerol
• Preservative: 0.02% Sodium Azide
• Purity: Immunogen affinity purified

Alternate Names for TPK1 Antibody - BSA Free

• EC 2.7.6.2
• HTPK1
• Placental protein 20
• PP20thiamine kinase
• thiamin pyrophosphokinase 1
• thiamine diphosphokinase
• Thiamine pyrophosphokinase 1

Background

TPK1 encodes a protein, that exists as a homodimer, which catalyzes the conversion of thiamine to thiamine pyrophosphate. Alternate transcriptional splice variants, encoding different isoforms, have been characterized.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for TPK1 Antibody (NBP1-83151)(1)

Publication using NBP1-83151

• Mayr JA, Freisinger P, Schlachter K et al. Thiamine pyrophosphokinase deficiency in encephalopathic children with defects in the pyruvate oxidation pathway. Am J Hum Genet 2011-12-01 [PMID: 22152682]

Bioinformatics

• Gene Symbol: TPK1