HIPK3 Antibody - BSA Free

Western Blot: HIPK3 Antibody [NBP2-24521] - Analysis of HIPK3 in mouse testis lysate in the 1) absence, 2) presence of immunizing peptide and 3) rat testis using this antibody at 1 ug/ml. Goat anti-rabbit Ig HRP secondary antibody and PicoTect ECL substrate solution were used for this test.

Immunocytochemistry/Immunofluorescence: HIPK3 Antibody [NBP2-24521] - HeLa cells were fixed for 10 minutes using 10% formalin and then permeabilized for 5 minutes using 1X TBS + 0.5% Triton-X100. The cells were incubated with anti-HIPK3 [NBP2-24521] at a 1:200 dilution overnight at 4C and detected with an anti-rabbit Dylight 488 (Green) at a 1:500 dilution. Alpha tubulin (DM1A) NB100-690 was used as a co-stain at a 1:1000 dilution and detected with an anti-mouse Dylight 550 (Red) at a 1:500 dilution. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 40X objective.

Immunohistochemistry-Paraffin: HIPK3 Antibody [NBP2-24521] - Analysis of human testis using this antibody at 10 ug/ml.

Immunohistochemistry-Paraffin: HIPK3 Antibody [NBP2-24521] - Analysis of HIPK3 in formalin-fixed, paraffin-embedded mouse testis tissue using an isotype control (top) and this antibody (bottom) at 5 ug/ml.

• Reactivity: Hu, Mu, Rt, Ca, Pm, Pm, Pm, Pm-Or, RM
• Applications: WB, ICC/IF, IHC
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: BSA Free
• Concentration: 1 mg/ml

HIPK3 Antibody - BSA Free Summary

• Description: Novus Biologicals Rabbit HIPK3 Antibody - BSA Free (NBP2-24521) is a polyclonal antibody validated for use in IHC, WB and ICC/IF. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: A portion of amino acids 850-900 of human HIPK3 was used as the immunogen.
• Predicted Species: Monkey (94%), Orangutan (94%), Chimpanzee (100%), Rhesus Macaque (94%). Backed by our 100% Guarantee.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: HIPK3
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence 1:200
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin 5-10 ug/ml
  • Western Blot 0.5-2 ug/ml

Reactivity Notes

Immunogen displays the following percentage of sequence identity for non-tested species: Equine (83%).

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS
• Preservative: 0.05% Sodium Azide
• Concentration: 1 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for HIPK3 Antibody - BSA Free

• Androgen receptor-interacting nuclear protein kinase
• DYRK6Fas-interacting serine/threonine-protein kinase
• EC 2.7.11.1
• FIST
• FIST3ANPK
• homeodomain interacting protein kinase 3
• homeodomain-interacting protein kinase 3
• PKYHomolog of protein kinase YAK1
• YAK1

Background

Seems to negatively regulate apoptosis by promoting FADD phosphorylation. Enhances androgen receptor-mediated transcription. May act as a transcriptional corepressor for NK homeodomain transcription factors

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: HIPK3
• Uniprot:
  • Q9H422(Human)