Home » HIPK3 » HIPK3 Antibodies » HIPK3 Antibody

HIPK3 Antibody

 Submit a Review

Images

 
(
Enlarge)
Western Blot: HIPK3 Antibody [NBP2-24521] - Analysis of HIPK3 in mouse testis lysate in the 1) absence, 2) presence of immunizing peptide and 3) rat testis using this antibody at 1 ug/ml. Goat anti-rabbit Ig HRP secondary antibody and PicoTect ECL substrate solution were used for this test.
Western Blot: HIPK3 Antibody [NBP2-24521] - Analysis of HIPK3 in mouse testis lysate in the 1) absence, 2) presence of immunizing peptide and 3) rat testis using this antibody at 1 ug/ml. Goat anti-rabbit Ig HRP ...read more
Immunocytochemistry/ Immunofluorescence: HIPK3 Antibody [NBP2-24521] - HeLa cells were fixed for 10 minutes using 10% formalin and then permeabilized for 5 minutes using 1X TBS + 0.5% Triton-X100. The cells were ...read more
Immunohistochemistry-Paraffin: HIPK3 Antibody [NBP2-24521] - Analysis of human testis using this antibody at 10 ug/ml.
Immunohistochemistry-Paraffin: HIPK3 Antibody [NBP2-24521] - Analysis of HIPK3 in formalin-fixed, paraffin-embedded mouse testis tissue using an isotype control (top) and this antibody (bottom) at 5 ug/ml.

Product Details

Summary
Reactivity Hu, Mu, Rt, Ca, PmSpecies Glossary
Applications WB, ICC/IF, IHC, IHC-P
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Concentration
1 mg/ml

Order Details

100% Guarantee on every product

HIPK3 Antibody Summary

Immunogen
A portion of amino acids 850-900 of human HIPK3 was used as the immunogen.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
HIPK3
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.
Learn about the Innovator's Reward

Applications/Dilutions

Dilutions
  • Western Blot 0.5-2 ug/ml
  • Immunocytochemistry/Immunofluorescence 1:200
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin 5-10 ug/ml
Control
HIPK3 Knockout HeLa Cell Lysate

Reactivity Notes

The amino acid sequence used as immunogen is 100% homologous in human (isoforms CRA_a and CRA_c) and chimpanzee, 94% homologous in orangutan and rhesus monkey, 83% homologous in horse and mouse, 77% homologous in cow.

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS
Preservative
0.05% Sodium Azide
Concentration
1 mg/ml
Purity
Immunogen affinity purified

Alternate Names for HIPK3 Antibody

  • Androgen receptor-interacting nuclear protein kinase
  • DYRK6Fas-interacting serine/threonine-protein kinase
  • EC 2.7.11.1
  • FIST
  • FIST3ANPK
  • homeodomain interacting protein kinase 3
  • homeodomain-interacting protein kinase 3
  • PKYHomolog of protein kinase YAK1
  • YAK1

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Customers Who Viewed This Item Also Viewed...

HIPK2 Antibody
(1F10)
H00028996-M03
Western Blot: HIPK2 Antibody (1F10) [H00028996-M03] - Analysis of HIPK2 expression in MES-SA/Dx5 (Cat # L021V1).
Immunohistochemistry-Paraffin: HIPK2 Antibody (1F10) [H00028996-M03] - Analysis of monoclonal antibody to HIPK2 on formalin-fixed paraffin-embedded human esophagus. Antibody concentration 1 ug/ml
Western Blot: HIPK2 Antibody (1F10) [H00028996-M03] - Analysis of HIPK2 expression in RIN-m5F.
Species: Hu, Rt
Applications: WB, ELISA, IHC, IHC-P
Androgen R/NR3C4
[p Ser213, p Ser210] ...
NB100-56603
Western Blot: Androgen R/NR3C4 [p Ser213, p Ser210] Antibody (156C135.2) [NB100-56603] - Analysis using Azide Free version of NB100-56603. LNCaP cells (passage number 38) were serum-starved for 2 days. After serum starvation, cells were (A) left untreated, (B) treated with 100 ng/ml IGF-1 for 4h, or (C) incubated with 20 um LY294002 for 30 mi
(12 Publications)
Species: Hu, Mu, Pm
Applications: WB, IHC, IHC-P
JNK1 Antibody
(228601) [Unconjugate
MAB17761
JNK1 was detected in immersion fixed MCF‑7 human breast cancer cell line using Mouse Anti-Human/Mouse/Rat JNK1 Monoclonal Antibody (Catalog # MAB17761) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # NL007) and counterstained with DAPI (blue). Specific staining was localized to cell surfaces. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.
Western blot shows lysates of CHP-100 human neuroblastoma cell line, C6 rat glioma cell line, and C2C12 mouse myoblast cell line. PVDF membrane was probed with 1 µg/mL Mouse Anti-Human/Mouse/Rat JNK1 Monoclonal Antibody (Catalog # MAB17761) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band for JNK1 was detected at approximately 46 kDa and 54 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 4.
Western blot shows recombinant human JNK1, JNK2, and JNK3 (1 ng/lane). PVDF membrane was probed with 1 µg/mL Mouse Anti-Human/Mouse/Rat JNK1 Monoclonal Antibody (Catalog # MAB17761) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band for JNK1 was detected at approximately 46 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 4.
(2 Reviews)
(3 Publications)
Species: Hu, Mu, Rt
Applications: WB, ICC
PIAS2 Antibody
NBP2-19819
Western Blot: PIAS2 Antibody [NBP2-19819] - Sample (30 ug of whole cell lysate) A: NIH-3T3 B: JC C: BCL-1 7. 5% SDS PAGE gel, diluted at 1:10000.
Immunohistochemistry-Paraffin: PIAS2 Antibody [NBP2-19819] - Immunohistochemical analysis of paraffin-embedded U87 xenograft, using antibody at 1:500 dilution.
Western Blot: PIAS2 Antibody [NBP2-19819] - Sample (30 ug of whole cell lysate) A: Jurkat B: Raji C: K562 D: THP-1 E: HL-60 F: NCI-H929 7. 5% SDS PAGE gel, diluted at 1:1000.
(1 Review)
Species: Hu, Mu
Applications: WB, IHC, IHC-P
Daxx Antibody
NB100-56136
Immunohistochemistry-Paraffin: Daxx Antibody - Unpurified [NB100-56136] - Daxx Antibody [NB100-56136] - Tissue sections of human prostate cancer (A and B) stained for Daxx expression using this antibody at 1:2000. Hematoxylin-eosin counterstain.
Species: Hu, Mu, Rt
Applications: WB, IHC, IHC-P, IP
c-jun Antibody
(5B1)
NBP2-37576
Western Blot: c-jun Antibody (5B1) [NBP2-37576] - Analysis using c-Jun mouse mAb against NIH/3T3 (1) and Cos7 (2) cell lysate.
Immunocytochemistry/Immunofluorescence: c-jun Antibody (5B1) [NBP2-37576] - Analysis of PC-2 cells using c-Jun mouse mAb (green). Red: Actin filaments have been labeled with Alexa Fluor-555 phalloidin.
Immunohistochemistry-Paraffin: c-jun Antibody (5B1) [NBP2-37576] - Analysis of human rectum cancer tissues using c-Jun mouse mAb with DAB staining.
Species: Hu, Mu, Pm
Applications: WB, ELISA, Flow, ICC/IF, IHC, IHC-P

SF-1/NR5A1/Steroidogen ...
NBP1-52823
Western Blot: SF-1/NR5A1/Steroidogenic Factor 1 Antibody [NBP1-52823] - Titration: 0.2-1 ug/ml, Positive Control: THP-1 cell lysate.
Immunohistochemistry-Paraffin: SF-1/NR5A1/Steroidogenic Factor 1 Antibody [NBP1-52823] - Human adrenal tissue at an antibody concentration of 4-8ug/ml.
(3 Publications)
Species: Hu
Applications: WB, ICC/IF, IHC, IHC-P
IL-2 [Unconjugated]
202-IL
1 μg/lane of Recombinant Human IL-2 was resolved with SDS-PAGE under reducing (R) conditions and visualized by silver staining, showing a single band at 13 kDa.
Recombinant Human IL-2 (Catalog # 202-IL) stimulates cell proliferation of the CTLL‑2 mouse cytotoxic T cell line. The ED50 for this effect is 0.05-0.25 ng/mL.
(18 Reviews)
(292 Publications)
Species: Hu
TPK1 Antibody
NBP1-83152
Western Blot: TPK1 Antibody [NBP1-83152] - Analysis in control (vector only transfected HEK293T lysate) and TPK1 over-expression lysate (Co-expressed with a C-terminal myc-DDK tag (3.1 kDa) in mammalian HEK293T cells).
Immunocytochemistry/Immunofluorescence: TPK1 Antibody [NBP1-83152] - Staining of human cell line CACO-2 shows localization to vesicles. Antibody staining is shown in green.
Immunohistochemistry-Paraffin: TPK1 Antibody [NBP1-83152] - Staining of human heart muscle shows moderate cytoplasmic positivity in myocytes.
Species: Hu
Applications: WB, ICC/IF, IHC, IHC-P
Ferredoxin
Reductase Antibody
NBP2-38530
Western Blot: Ferredoxin Reductase Antibody [NBP2-38530] - Analysis using Anti-FDXR antibody NBP2-38530 (A) shows similar pattern to independent antibody NBP2-38706 (B).
Immunocytochemistry/Immunofluorescence: Ferredoxin Reductase Antibody [NBP2-38530] - Staining of human cell line U-2 OS shows positivity in mitochondria. Antibody staining is shown in green.
Immunohistochemistry-Paraffin: Ferredoxin Reductase Antibody [NBP2-38530] - Staining of human ovary.
Species: Hu
Applications: WB, ICC/IF, IHC, IHC-P
FADD [p Ser194]
Antibody (709736)
MAB7047
Western blot shows lysates of HT‑29 human colon adenocarcinoma cell line and HeLa human cervical epithelial carcinoma cell line untreated (-) or treated (+) with 1 µg/mL nocodazole or 4 mM hydroxyurea for 20 hours. PVDF membrane was probed with 1 µg/mL of Mouse Anti-Human Phospho-FADD (S194) Monoclonal Antibody (Catalog # MAB7047) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band was detected for FADD at approximately 28 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
FADD phosphorylated at S194 was detected in immersion fixed HeLa human cervical epithelial carcinoma cell line stimulated with nocadazole using Mouse Anti-Human Phospho-FADD (S194) Monoclonal Antibody (Catalog # MAB7047) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # NL007) and counterstained with DAPI (blue). Specific staining was localized to nuclei. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.
Species: Hu
Applications: WB, ICC
HIPK1 Antibody
AF5617
Western blot shows lysates of 293T human embryonic kidney cell line and HeLa human cervical epithelial carcinoma cell line. PVDF membrane was probed with 1 µg/mL of Human HIPK1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5617) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). A specific band was detected for HIPK1 at approximately 130 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Species: Hu
Applications: WB
CYP11A1 Antibody
NBP1-85368
Western Blot: CYP11A1 Antibody [NBP1-85368] - protein extract from: 1) human liver (negative), 2) human testis (positve), 3) MW marker (drawn with special ECL reagent marker), 4) human testis, 5) H295R adrenal cell line. Image submitted by a verified customer review.
Immunocytochemistry/Immunofluorescence: CYP11A1 Antibody [NBP1-85368] - CYP11A1 expression (Leydig cells) present in early pubertal Rhesus monkey. Secondary antibody: Alexa Fluor488 anti rabbit. Image submitted by a verified customer review.
Immunohistochemistry-Paraffin: CYP11A1 Antibody [NBP1-85368] - Staining of human cerebral cortex shows low expression as expected.
(4 Reviews)
(1 Publication)
Species: Hu, RM
Applications: WB, ICC/IF, IHC, IHC-P
MYLIP Antibody
NBP1-54903
Western Blot: MYLIP Antibody [NBP1-54903] - Titration: 0.2-1 ug/ml, Positive Control: SK-MEL-2 cell lysate.
Species: Hu
Applications: WB
Supplier Logo

Publications for HIPK3 Antibody (NBP2-24521) (0)

There are no publications for HIPK3 Antibody (NBP2-24521).
By submitting your publication information earn gift cards and discounts for future purchases.

Reviews for HIPK3 Antibody (NBP2-24521) (0)

There are no reviews for HIPK3 Antibody (NBP2-24521). By submitting a review you will receive an Amazon e-Gift Card or Novus Product Discount.
  • Review with no image -- $10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen
  • Review with an image -- $25/€18/£15/$25 CAD/¥150 Yuan/¥2500 Yen

Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for HIPK3 Antibody (NBP2-24521) (0)

There are no specific FAQs related to this product. Read our general customer & technical service FAQs.

Control Lysate(s)

HIPK3 Knockout HeLa Cell Lysate

Secondary Antibodies

 

Isotype Controls

Other Available Formats

Learn about Custom Antibody Labeling

Additional HIPK3 Products

Array NBP2-24521

Bioinformatics Tool for HIPK3 Antibody (NBP2-24521)

Discover related pathways, diseases and genes to HIPK3 Antibody (NBP2-24521). Need help? Read the Bioinformatics Tool Guide for instructions on using this tool.
Visit Tool

Diseases for HIPK3 Antibody (NBP2-24521)

Discover more about diseases related to HIPK3 Antibody (NBP2-24521).
 

Pathways for HIPK3 Antibody (NBP2-24521)

View related products by pathway.

PTMs for HIPK3 Antibody (NBP2-24521)

Learn more about PTMs related to HIPK3 Antibody (NBP2-24521).
 

Research Areas for HIPK3 Antibody (NBP2-24521)

Find related products by research area.

Blogs on HIPK3

There are no specific blogs for HIPK3, but you can read our latest blog posts.
Recombinant Monoclonal Antibodies

Customers Who Bought This Also Bought

Contact Information

Learn the difference between western blot and simple western

Product PDFs

  SDS
  PDF Datasheet
  Request CofA

Calculators

Concentration Calculator

The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.

=
÷

Review this Product

Be the first to review our HIPK3 Antibody and receive a gift card or discount.


Bioinformatics

Gene Symbol HIPK3
Uniprot
COVID-19 update