TopBP1 Antibody [Alexa Fluor® 594]

• Reactivity: Hu
• Applications: IHC
• Clonality: Polyclonal
• Host: Sheep
• Conjugate: Alexa Fluor 594

TopBP1 Antibody [Alexa Fluor® 594] Summary

• Immunogen: E. coli-derived recombinant human TopBP1
  Met88-Asp194
  Accession # Q92547
• Specificity: Detects human TopBP1 in direct ELISAs.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Sheep
• Purity Statement: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry

Packaging, Storage & Formulations

• Storage: Protect from light. Do not freeze. 12 months from date of receipt, 2 to 8 °C as supplied
• Buffer: Supplied 0.2mg/ml in 1X PBS with RDF1 and 0.09% Sodium Azide

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for TopBP1 Antibody [Alexa Fluor® 594]

• DNA topoisomerase II-beta-binding protein 1
• DNA topoisomerase II-binding protein 1
• KIAA0259DNA topoisomerase 2-binding protein 1
• TOP2BP1
• TopBP1
• topoisomerase (DNA) II binding protein 1

Background

TopBP1 (DNA topoisomerase 2-binding protein 1) is a 175-180 kDa member of a critical DNA replication repair complex. It is widely expressed, and appears to serve as an intermediary in DNA replication. When a replication fork is stalled during S phase, DNA continues to unwind ahead of the stall. This becomes associated with RPA, which is essential for ATR and subsequent Chk1 activation. TopBP1 is key to ATR activation, and appears to first bind to DNA:pol-alpha , followed by a 9-1-1 complex and Rad17 interaction that leads to ATR stimulation. Human TopBP1 is 1522 amino acids (aa) in length. It contains eight BRCT domains (aa 101-1486) that interact with distinct binding partners. BRCT1 and 2 binds to Rad9 (aa 101-294), while BRCT 6 and 7 are involved in ATR activation. There are 12 utilized phosphorylation sites and two nuclear localization signals. Three isoform variants have been described. One contains an alternative start site at Met88, a second shows an 18 aa substitution for aa 1198-1522, and a third possesses a six aa substitution for aa 1292-1522. Over aa 88-194, human TopBP1 shares 88% aa identity with mouse TopBP1.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.