TOP2A Antibody (rTOP2A/8339) - Azide and BSA Free

Formalin-fixed, paraffin-embedded human tonsil stained with TOP2A Antibody (rTOP2A/8339) - Azide and BSA Free. Inset: PBS instead of primary antibody; secondary only negative control.

• Reactivity: Hu
• Applications: IHC
• Clone: rTOP2A/8339
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Format: Azide and BSA Free

TOP2A Antibody (rTOP2A/8339) - Azide and BSA Free Summary

• Description: Prepared in 10mM PBS WITHOUT BSA & azide at 1.0mg/ml. Also available with 0.05% BSA & 0.05% azide.
  
  Antibody with azide - store at 2 to 8 C. Antibody without azide - store at -20 to -80 C. Antibody is stable for 24 months. Non-hazardous.
• Additional Information: Recombinant Monoclonal Antibody
• Immunogen: Recombinant human Topoisomerase II alpha fragment (aa1300-1500) (exact sequence is proprietary) (Uniprot: P11388)
• Localization: Nucleus
• Specificity: It recognizes a 170kDa protein, which is identified as topoisomerase II. It shows no cross-reaction with Topoisomerase II or I.
• Isotype: IgG1 Kappa
• Clonality: Monoclonal
• Host: Mouse
• Gene: TOP2A
• Purity: Protein A or G purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry
• Theoretical MW: 170 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at -20 to -80C. Avoid freeze-thaw cycles.
• Buffer: 10 mM PBS
• Preservative: No Preservative
• Purity: Protein A or G purified

Alternate Names for TOP2A Antibody (rTOP2A/8339) - Azide and BSA Free

• DNA topoisomerase (ATP-hydrolyzing)
• DNA topoisomerase 2-alpha
• DNA topoisomerase II, 170 kD
• DNA topoisomerase II, alpha isozyme
• EC 5.99.1.3
• TOP2
• TOP2A
• TOP2DNA gyrase
• topoisomerase (DNA) II alpha (170kD)
• topoisomerase (DNA) II alpha 170kDa
• TP2A

Background

Topo II Alpha encodes a DNA topoisomerase, an enzyme that controls and alters the topologic states of DNA during transcription. This nuclear enzyme is involved in processes such as chromosome condensation, chromatid separation, and the relief of torsional stress that occurs during DNA transcription and replication. It catalyzes the transient breaking and rejoining of two strands of duplex DNA which allows the strands to pass through one another, thus altering the topology of DNA. Two forms of this enzyme exist as likely products of a gene duplication event. The gene encoding this form, alpha, is localized to chromsome 17 and the beta gene is localized to chromosome 3. The gene encoding this enzyme functions as the target for several anticancer agents and a variety of mutations in this gene have been associated with the development of drug resistance. Reduced activity of this enzyme may also play a role in ataxia-telangiectasia.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: TOP2A