TOP2A Antibody (1E2) - Azide and BSA Free

Western Blot: TOP2A Antibody (1E2) [H00007153-M01] - TOP2A monoclonal antibody (M01), clone 1E2 Analysis of TOP2A expression in Hela S3 NE.

Immunocytochemistry/Immunofluorescence: TOP2A Antibody (1E2) [H00007153-M01] - Analysis of monoclonal antibody to TOP2A on HeLa cell. Antibody concentration 10 ug/ml.

Immunohistochemistry-Paraffin: TOP2A Antibody (1E2) [H00007153-M01] - Analysis of monoclonal antibody to TOP2A on formalin-fixed paraffin-embedded human testis. Antibody concentration 3 ug/ml.

ELISA: TOP2A Antibody (1E2) [H00007153-M01] - Detection limit for recombinant GST tagged TOP2A is approximately 0.3ng/ml as a capture antibody.

• Reactivity: Hu
• Applications: WB, ELISA, ICC/IF, IHC
• Clone: 1E2
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Format: Azide and BSA Free

TOP2A Antibody (1E2) - Azide and BSA Free Summary

• Description: Quality control test: Antibody Reactive Against Recombinant Protein.
• Immunogen: TOP2A (NP_001058, 1435 a.a. ~ 1531 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. RAAPKGTKRDPALNSGVSQKPDPAKTKNRRKRKPSTSDDSDSNFEKIVSKAVTSKKSKGESDDFHMDFDSAVAPRAKSVRAKKPIKYLEESDEDDLF
• Localization: Cytoplasmic and Nuclear; nucleoplasm. Note: Generally located in the nucleoplasm.
• Specificity: TOP2A - topoisomerase (DNA) II alpha 170kDa
• Isotype: IgG1 Kappa
• Clonality: Monoclonal
• Host: Mouse
• Gene: TOP2A
• Purity: IgG purified

Applications/Dilutions

• Dilutions:
  • ELISA
  • Immunocytochemistry/ Immunofluorescence
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin
  • Western Blot
• Application Notes: Antibody reactivity against cell lysate and recombinant protein for WB. It has also been used for IF, IHC-P and ELISA.

Packaging, Storage & Formulations

• Storage: Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
• Buffer: In 1x PBS, pH 7.4
• Preservative: No Preservative
• Purity: IgG purified

Notes

This product is produced by and distributed for Abnova, a company based in Taiwan.

Alternate Names for TOP2A Antibody (1E2) - Azide and BSA Free

• DNA topoisomerase (ATP-hydrolyzing)
• DNA topoisomerase 2-alpha
• DNA topoisomerase II, 170 kD
• DNA topoisomerase II, alpha isozyme
• EC 5.99.1.3
• TOP2
• TOP2A
• TOP2DNA gyrase
• topoisomerase (DNA) II alpha (170kD)
• topoisomerase (DNA) II alpha 170kDa
• TP2A

Background

This gene encodes a DNA topoisomerase, an enzyme that controls and alters the topologic states of DNA during transcription. This nuclear enzyme is involved in processes such as chromosome condensation, chromatid separation, and the relief of torsional stress that occurs during DNA transcription and replication. It catalyzes the transient breaking and rejoining of two strands of duplex DNA which allows the strands to pass through one another, thus altering the topology of DNA. Two forms of this enzyme exist as likely products of a gene duplication event. The gene encoding this form, alpha, is localized to chromsome 17 and the beta gene is localized to chromosome 3. The gene encoding this enzyme functions as the target for several anticancer agents and a variety of mutations in this gene have been associated with the development of drug resistance. Reduced activity of this enzyme may also play a role in ataxia-telangiectasia.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for TOP2A Antibody (H00007153-M01)(1)

Publication using H00007153-M01

• Poonperm R, Takata H, Hamano T et al. Chromosome Scaffold is a Double-Stranded Assembly of Scaffold Proteins. Sci Rep. 2015-07-03 [PMID: 26132639]

Bioinformatics

• Gene Symbol: TOP2A
• Entrez:
  • 7153(Human)
• OMIM:
  • 126430(Human)
• Uniprot:
  • NP_001058(Human)