TOM70 Antibody [HRP]

Product Discontinued

TOM70 Antibody [HRP] Summary

• Immunogen: A synthetic peptide made to a region within residues 1-100 of the human TOM70 protein. [Swiss-Prot# O94826]
• Localization: Mitochondrial; multi-pass membrane.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: TOMM70
• Purity: Protein A purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence
  • Western Blot
• Application Notes: This TOM70 antibody is useful for Immunocytochemistry/Immunofluorescence and Western blot, where a band is seen at ~67 kDa. In ICC/IF mitochondrial staining was observed in MCF7 cells.
  
  The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
• Theoretical MW: 67 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
• Readout System:
  • NovaLume Plus Chemiluminescent Reagent LUM-555

Reactivity Notes

Rabbit reactivity reported in scientific literature (PMID: 22292033).

Packaging, Storage & Formulations

• Storage: Store at 4C in the dark.
• Buffer: PBS
• Preservative: No Preservative
• Purity: Protein A purified

Alternate Names for TOM70 Antibody [HRP]

• FLJ90470
• KIAA0719
• mitochondrial import receptor subunit TOM70
• Mitochondrial precursor proteins import receptor
• TOM70
• Translocase of outer membrane 70 kDa subunit
• translocase of outer mitochondrial membrane 70 (yeast) homolog A
• translocase of outer mitochondrial membrane 70 homolog A (S. cerevisiae)
• translocase of outer mitochondrial membrane 70 homolog A (yeast)

Background

Functional mitochondria require up to 1000 proteins to function properly, with 99% synthesized as precursors in the cytoplasm and transported into the mitochondria with the aid of cytosolic chaperones and mitochondrial translocators (import components). Proteins to be imported are chaperoned to the mitochondria by the cytosolic heat shock protein (cHSP70) and are immediately pursued by Translocators of the Outer Membrane (TOMs), followed by transient interactions of the unfolded proteins with Translocators of the Inner Membrane (TIMs). TOMM70A is ubiquitously expressed in human tissues and localizes in the mitochondria. TOMM70A could play a significant role in the import of nuclear-encoded mitochondrial proteins with internal targeting sites such as ADP/ATP carriers and the uncoupling proteins.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: TOMM70