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TOM1L2 293T Cell Transient Overexpression Lysate

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TOM1L2 transfected lysate.
TOM1L2 transfected lysate.
Lane 1: TOM1L2 transfected lysate (50.38 KDa) Lane 2: Non-transfected lysate.

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB

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TOM1L2 293T Cell Transient Overexpression Lysate Summary

Description
Plasmid: pCMV-TOM1L2 full-length
Specificity
TOM1L2 293T Cell Transient Overexpression Lysate(Denatured)
Gene
TOM1L2

Applications/Dilutions

Dilutions
  • Western Blot

Packaging, Storage & Formulations

Storage
Store at -80C. Avoid freeze-thaw cycles.
Buffer
1X Sample Buffer (50 mM Tris-HCl, 2% SDS, 10% glycerol, 300 mM 2-mercaptoethanol, 0.01% Bromophenol blue)

Lysate Details for Array

Type
293T Cell Transient Overexpression
Protein State
Denatured

Notes

Quality control test: Transient overexpression cell lysate was tested with Anti-TOM1L2 antibody by Western Blots.

This product is produced by and distributed for Abnova, a company based in Taiwan.

Alternate Names for TOM1L2 293T Cell Transient Overexpression Lysate

  • FLJ21501
  • FLJ32746
  • target of myb1 (chicken) homolog-like 1
  • target of myb1-like 2 (chicken)
  • Target of Myb-like protein 2
  • TOM1-like protein 2

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Lysates are guaranteed for 6 months from date of receipt.

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NBP1-86170
Western Blot: TOM1 Antibody [NBP1-86170] - Lane 1: Marker [kDa] 207, 110, 79, 49, 32, 25, 17. Lane 2: Human cell line RT-4. Lane 3: Human cell line EFO-21. Lane 4: Human cell line A-431. Lane 5: Human liver tissue. Lane 6: Human tonsil tissue
Immunohistochemistry-Paraffin: TOM1 Antibody [NBP1-86170] - Staining of human kidney shows moderate cytoplasmic positivity in cells in tubules.
Immunohistochemistry-Paraffin: TOM1 Antibody [NBP1-86170] - Staining of human endometrium shows moderate cytoplasmic positivity in glandular cells.
Species: Hu
Applications: IHC, IHC-P, WB
TOM1L1 Antibody
NBP2-20680
Western Blot: TOM1L1 Antibody [NBP2-20680] - Non-transfected (-) and transfected (+) 293T whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with TOM1L1 Antibody diluted at 1:5000. HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
Western Blot: TOM1L1 Antibody [NBP2-20680] - Sample (30 ug of whole cell lysate) A: 293T 10% SDS PAGE gel, diluted at 1:10000.
Immunohistochemistry-Paraffin: TOM1L1 Antibody [NBP2-20680] - Immunohistochemical analysis of paraffin-embedded Hepatoma, using antibody at 1:500 dilution.
Species: Hu
Applications: IHC, IHC-P, WB
HGS Antibody (6D11)
H00009146-M01
Immunocytochemistry/Immunofluorescence: HGS Antibody (6D11) [H00009146-M01] - Analysis of monoclonal antibody to HGS on HeLa cell. Antibody concentration 10 ug/ml.
Western Blot: HGS Antibody (6D11) [H00009146-M01] - Analysis of HGS over-expressed 293 cell line, cotransfected with HGS Validated Chimera RNAi ( Cat # H00009146-R01V ) (Lane 2) or non-transfected control (Lane 1). Blot probed with HGS monoclonal antibody (M01), clone 6D11 (Cat # H00009146-M01 ). GAPDH ( 36.1 kDa ) used as specificity and loading control.
Sandwich ELISA: HGS Antibody (6D11) [H00009146-M01] - Detection limit for recombinant GST tagged HGS is approximately 0.03ng/ml as a capture antibody.
(2 Publications)
Species: Hu, Mu
Applications: ELISA, Flow, ICC/IF, KD, S-ELISA, WB
STAM-1 Antibody
NBP2-38514
Immunohistochemistry-Paraffin: STAM-1 Antibody [NBP2-38514] - Staining of human cerebral cortex shows moderate cytoplasmic positivity in neurons.
Immunohistochemistry-Paraffin: STAM-1 Antibody [NBP2-38514] - Staining of human testis shows moderate cytoplasmic positivity in cells in seminiferous ducts and leydig cells.
Immunohistochemistry-Paraffin: STAM-1 Antibody [NBP2-38514] - Staining of human small intestine shows moderate cytoplasmic positivity in glandular cells.
Species: Hu
Applications: ICC/IF, IHC, IHC-P, WB
SFRS5 Antibody
H00006430-B01P
Immunohistochemistry: SFRS5 Antibody [H00006430-B01P] - Representative images from immune-histochemical staining of Ac-SRSF5, SRSF5, and Smurf1 in three serial sections of the same tumor and matched adjacent tissue. Scale bar, 50um. Image collected and cropped by CiteAb from the following publication (nature.com/articles/s41467-018-04815-3), licensed under a CC-BY licence.
Immunocytochemistry/Immunofluorescence: SFRS5 Antibody [H00006430-B01P] - Analysis of purified antibody to SFRS5 on HeLa cell. (antibody concentration 10 ug/ml)
Immunohistochemistry-Paraffin: SFRS5 Antibody [H00006430-B01P] - Analysis of purified antibody to SFRS5 on formalin-fixed paraffin-embedded human ovary. (antibody concentration 1.5 ug/ml)
(1 Review)
(2 Publications)
Species: Hu
Applications: ICC/IF, IHC, IHC-P, WB
ATN1 Antibody
NBP1-90044
Immunocytochemistry/Immunofluorescence: ATN1 Antibody [NBP1-90044] - Staining of human cell line U-251 MG shows localization to nucleoplasm. Antibody staining is shown in green.
Immunohistochemistry-Paraffin: ATN1 Antibody [NBP1-90044] - Analysis in human cerebral cortex and liver tissues. Corresponding ATN1 RNA-seq data are presented for the same tissues.
Immunohistochemistry-Paraffin: ATN1 Antibody [NBP1-90044] - Staining of human placenta shows strong nuclear positivity in trophoblastic cells.
(1 Publication)
Species: Hu
Applications: ICC/IF, IHC, IHC-P, WB
HARS Antibody
NBP1-89490
Immunocytochemistry/Immunofluorescence: HARS Antibody [NBP1-89490] - Staining of human cell line A-431 shows localization to cytosol. Antibody staining is shown in green.
Immunohistochemistry-Paraffin: HARS Antibody [NBP1-89490] - Staining of human cerebral cortex shows cytoplasmic positivity in neuronal cells.
Immunohistochemistry-Paraffin: HARS Antibody [NBP1-89490] - Staining of human skin shows positivity in epidermal cells.
Species: Hu, Mu, Rt
Applications: ICC/IF, IHC, IHC-P, KD, WB
TSG101 Antibody
(4A10)
NB200-112
Western Blot: TSG101 Antibody (4A10) [NB200-112] - Lane 1: MWM, Lane 2: TSG101 in RAW 264.7 Whole Cell Lysate, Lane 3: Exosome Lysate. Used primary antibody at 1:500 and Donkey Anti-Mouse at 0.5 ug/mL (this could be titrated down to reduce background). Used picogram sensitivity ECL reagents. Image from verified customer review.
Flow Cytometry: TSG101 Antibody (4A10) [NB200-112] - An intracellular stain was performed on HeLa cells with TSG101 Antibody (4A10) NB200-112F (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeabilized with 0.1% saponin. Cells were incubated in an antibody dilution of 5 ug/mL for 30 minutes at room temperature. Both antibodies were conjugated to FITC.
Flow Cytometry: TSG101 Antibody (4A10) [NB200-112] - An intracellular stain was performed on Hek293 cells with TSG101 Antibody [4A10] NB200-112PE (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeablized with 0.1% saponin. Cells were incubated in an antibody dilution of 2.5 ug/mL for 30 minutes at room temperature. Both antibodies were conjugated to Phycoerythrin.
(2 Reviews)
(89 Publications)
Species: Ca, Ha, Hu, Pm, Mu, Po, Pm, Rt
Applications: EM, ELISA, Flow, ICC/IF, IHC-FrFl, IHC, IHC-P, IP, KD, WB
GLYAT Antibody
NBP2-58917
Western Blot: GLYAT Antibody [NBP2-58917] - Western blot analysis in human cell line RT-4, human cell line U-251 MG, human plasma and human liver tissue.
Immunohistochemistry-Paraffin: GLYAT Antibody [NBP2-58917] - Staining of human duodenum shows low expression as expected.
Immunohistochemistry-Paraffin: GLYAT Antibody [NBP2-58917] - Staining of human kidney shows high expression.
Species: Hu
Applications: IHC, IHC-P, WB
c-Myc Antibody
(9E10) - BSA Free
NB600-302
Flow Cytometry: c-Myc Antibody (9E10) [NB600-302] - An intracellular stain was performed on U-937 cells with c-Myc Antibody (9E10) NB600-302AF488 (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeabilized with 0.1% saponin. Cells were incubated in an antibody dilution of 10 ug/mL for 30 minutes at room temperature. Both antibodies were conjugated to Alexa Fluor 488.
Flow Cytometry: c-Myc Antibody (9E10) [NB600-302] - An intracellular stain was performed on Jurkat cells with c-Myc Antibody (9E10) NB600-302R (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeabilized with 0.1% saponin. Cells were incubated in an antibody dilution of 5 ug/mL for 30 minutes at room temperature. Both antibodies were conjugated to DyLight 550.
Western Blot: c-Myc Antibody (9E10) [NB600-302] - CtIP is phosphorylated by CDKs at multiple sites. Myc-BRCA1 and/or HA-CtIP WT or indicated mutants were expressed in 293T cells and co-immunoprecipitation was performed. Image collected and cropped by CiteAb from the following publication (//doi.org/10.1371/journal.pgen.1003277) licensed under a CC-BY licence.
(4 Reviews)
(38 Publications)
Species: Bv, Dr, Hu, Mu
Applications: ChIP, ELISA, Flow-IC, Flow, IB, ICC/IF, IHC, IHC-Fr, IHC-P, IP, PLA, S-ELISA, Simple Western, WB
NCOR1 Antibody
NB100-58824
Western Blot: NCOR1 Antibody [NB100-58824] - Whole cell lysate (50 ug) from HEK293T, K-562, and NIH 3T3 cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-NCoR antibodyused for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 3minutes.
Immunoprecipitation: NCOR1 Antibody [NB100-58824] - Detection of Human NCOR on HeLa whole cell lysate using NB100-5882. NCOR was also immunoprecipitated by rabbit anti-NCOR antibody NB100-58825.
Chromatin Immunoprecipitation Sequencing: NCOR1 Antibody [NB100-58824] - Localization of NCoR Binding Sites by ChIP-sequencing. Chromatin from K562 cells was immunoprecipitated with anti-NCoR antibody NB100-58824 and analyzed by DNA sequencing. The figure illustrates the peak distribution of NCoR binding within a 650 Kb region of the human X chromosome as detected using anti-NCoR antibody NB100-58824. ChIP-seq validation performed by Diogenode, Denville, NJ.
(3 Publications)
Species: Hu, Mu
Applications: CHIP-SEQ, ChIP, IP, WB
Tollip Antibody
NBP1-81597
Western Blot: Tollip Antibody [NBP1-81597] - Analysis in human cerebral cortex tissue.
Immunocytochemistry/Immunofluorescence: Tollip Antibody [NBP1-81597] - Immunofluorescent staining of human cell line U-2 OS shows localization to plasma membrane & cytosol.
Western Blot: Tollip Antibody [NBP1-81597] - Analysis in mouse cell line NIH-3T3 and rat cell line NBT-II.
(2 Publications)
Species: Hu, Rt
Applications: ICC/IF, IHC, IHC-P, KD, WB
SREBP1 Antibody
(2A4) - BSA Free
NB600-582
Western Blot: SREBP1 Antibody (2A4) [NB600-582] - Hepatic SREBP1c and SREBP2 protein abundance in C57BL/6 mice assigned to a high-fat (HF) or a high-fat/exercise (HF + EX) group for 8 weeks. Cytosolic and nuclear SREBP1c abundance. Image collected and cropped by CiteAb from the following publication (http://www.hindawi.com/journals/jl/2013/908048/), licensed under a CC-BY licence.
Knockout Validated: SREBP1 Antibody (2A4) [NB600-582] - KO and OE of SREBP1 influenced lipid metabolism in Bel-7402 cells. The protein level of lipid-associated genes in SREBP1-KO, SREBP1-OE, and WT Bel-7402 cells. Data were expressed as mean -/+ standard error of the mean. *P < 0.05, **P < 0.01, and ***P < 0.001 vs. WT group, respectively.
Simple Western: SREBP1 Antibody (2A4) [NB600-582] - Lane view shows a specific band for SREBP1 in 1.0 mg/ml of HeLa lysate. This experiment was performed under reducing conditions using the 12-230 kDa separation system.
(46 Publications)
Species: Ca, Ch, SyHa, Ha, Hu, Pm, Mu, Rt
Applications: IHC-Fr, KO, Simple Western, WB
Fyn Antibody
(FYN-01) - BSA Free
NB500-517
Immunoprecipitation: Fyn Antibody (FYN-01) [NB500-517] - Immunoprecipitation of Fyn from the lysate of T cells isolated from fresh buffy coats. Western blot was immunostained with anti-Fyn (FYN-01). Lane 1: original lysate of T cells Lane 2-3: Immunoprecipitated material eluted from affinity sorbent (FYN-01 coupled to Sepharose beads). Lanes differ in amount of T cell lysate loaded on the immunosorbent.
Immunocytochemistry/Immunofluorescence: Fyn Antibody (FYN-01) [NB500-517] - Staining of Fyn in human primary fibroblasts using anti-Fyn (FYN-01; green). Actin cytoskeleton was decorated by phalloidin (red) and cell nuclei stained with DAPI (blue).
Immunocytochemistry/Immunofluorescence: Fyn Antibody (FYN-01) [NB500-517] - Staining of Fyn in human HeLa cell line using anti-Fyn (FYN-01; green). Actin cytoskeleton was decorated by phalloidin (red) and cell nuclei stained with DAPI (blue).
(1 Review)
(4 Publications)
Species: Hu, Mu
Applications: ICC/IF, IHC, IHC-P, IP, WB
p53 Antibody (PAb
240) - BSA Free
NB200-103
Flow Cytometry: p53 Antibody (PAb 240) [NB200-103] - An intracellular stain was performed on HeLa cells with p53 (PAb240) NB200-103AF647 (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeablized with 0.1% saponin. Cells were incubated in an antibody dilution of 2.5 ug/mL for 30 minutes at room temperature. Both antibodies were conjugated to Alexa Fluor 647.
Flow Cytometry: p53 Antibody (PAb 240) [NB200-103] - An intracellular stain was performed on A549 cells with p53 (PAb240) NB200-103AF488 (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeablized with 0.1% saponin. Cells were incubated in an antibody dilution of 10 ug/mL for 30 minutes at room temperature. Both antibodies were conjugated to Alexa Fluor 488.
Flow (Intracellular): p53 Antibody (PAb 240) [NB200-103] - An intracellular stain was performed on HeLa cells with p53 (PAb240) NB200-103 (blue) and a matched isotype control NBP2-27287 (orange). Cells were fixed with 4% PFA and then permeablized with 0.1% saponin. Cells were incubated in an antibody dilution of 2.5 ug/mL for 30 minutes at room temperature, followed by APC-conjugated anti-mouse IgG secondary antibody F0101B.
(3 Reviews)
(33 Publications)
Species: Hu, Mu, Rt, Xp(-), Ye
Applications: CyTOF-ready, ELISA, Flow-IC, Flow, ICC/IF, IHC, IHC-Fr, IHC-P, IP, WB
COP9 Antibody
(JE47-48)
NBP2-80456
Western Blot: COP9 Antibody (JE47-48) [NBP2-80456] - Analysis of COPS3/CSN3 on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (1:500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:5,000 dilution was used for 1 hour at room temperature. Positive control: Lane 1: Human skeletal muscle tissue lysate Lane 2: Rat bone marrow lysate.
Immunocytochemistry/Immunofluorescence: COP9 Antibody (JE47-48) [NBP2-80456] - ICC staining of COPS3/CSN3 in A431 cells (green). Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 1% Blocker BSA for 15 minutes at room temperature. Cells were probed with the primary antibody (1:500) for 1 hour at room temperature, washed with PBS. Alexa Fluor 488 Goat anti-Rabbit IgG was used as the secondary antibody at 1:100 dilution. The nuclear counter stain is DAPI (blue).
Immunocytochemistry/Immunofluorescence: COP9 Antibody (JE47-48) [NBP2-80456] - ICC staining of COPS3/CSN3 in HCT116 cells (green). Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 1% Blocker BSA for 15 minutes at room temperature. Cells were probed with the primary antibody (1:500) for 1 hour at room temperature, washed with PBS. Alexa Fluor 488 Goat anti-Rabbit IgG was used as the secondary antibody at 1:100 dilution. The nuclear counter stain is DAPI (blue).
Species: Hu, Mu, Rt
Applications: Flow, ICC/IF, IHC, IHC-P, WB
PXMP2
Overexpression Lysate
NBL1-15012
Western Blot: PXMP2 Overexpression Lysate (Adult Normal) [NBL1-15012] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for PXMP2.
Species: Hu
Applications: WB
ABCA1 Antibody -
BSA Free
NB400-105
Dual RNAscope ISH-IHC: ABCA1 Antibody [NB400-105] - Formalin-fixed paraffin-embedded tissue sections of human prostate cancer were probed for ABCA1 mRNA (ACD RNAScope Probe, catalog # 432291; Fast Red chromogen, ACD catalog # 322360). Adjacent tissue section was processed for immunohistochemistry using rabbit polyclonal (Novus Biologicals catalog # NB400-105) at 1.5ug/mL with overnight incubation at 4 degrees Celsius followed by incubation with anti-rabbit IgG VisUCyte HRP Polymer Antibody (Catalog # VC003) and DAB chromogen (yellow-brown). Tissue was counterstained with hematoxylin (blue). Specific staining was localized to glandular cells.
Immunohistochemistry-Paraffin: ABCA1 Antibody [NB400-105] - Detection of ABCA1 in human prostate epithelium showing luminal and membrane staining.
Western Blot: ABCA1 Antibody [NB400-105] - Analysis of ABCA1 in total cell lysates of RAW264.9 cells treated with vehicle (-) or 9-cisretinoic acid and 22Rhydroxycholesterol (+). Samples used for this testing were 40 ug of total cell post-nuclear lysate from each group.
(7 Reviews)
(372 Publications)
Species: Ca, Ch, ChHa, Eq, Ha, Hu, Mu, Md, Po, Pm, Rb, Rt
Applications: B/N, ChIP, Dual ISH-IHC, ELISA, Flow, GS, IB, ICC/IF, IHC, IHC-Fr, IHC-P, IP, KD, KO, PCR, Simple Western, WB
MTCH1 Antibody
NBP2-93766
Western Blot: MTCH1 Antibody [NBP2-93766] - Analysis of extracts of various cell lines, using MTCH1 .Exposure time: 30s.
Immunohistochemistry-Paraffin: MTCH1 Antibody [NBP2-93766] - Paraffin-embedded human breast cancer using MTCH1 .
Species: Hu, Mu, Rt
Applications: IHC, IHC-P, WB
CASK Antibody -
BSA Free
NBP2-41181
Western Blot: CASK Antibody [NBP2-41181] - Western blot analysis of CASK in mouse brain tissue lysate with CASK antibody at 1 ug/ml in (A) the absence and (B) the presence of blocking peptide.
Simple Western: CASK Antibody [NBP2-41181] - 0.6 mg/ml protein lysates from differentiating human neuroepithelial stem cells run on Simple Western with 1:500 CASK.Ab (blue) and 1:1000 CASK.Ab (pink) and an actin antibody as positive control. The CASK peak is located at 116 kDa and actin at 38 kDa. Image is courtesy of customer.
Immunocytochemistry/Immunofluorescence: CASK Antibody [NBP2-41181] - Immunofluorescence of CASK in human brain tissue with CASK antibody at 20 ug/mL.
(1 Review)
(2 Publications)
Species: Hu, Mu, Rt
Applications: ELISA, ICC/IF, Simple Western, WB

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Dual RNAscope ISH-IHC: ABCA1 Antibody [NB400-105] - Formalin-fixed paraffin-embedded tissue sections of human prostate cancer were probed for ABCA1 mRNA (ACD RNAScope Probe, catalog # 432291; Fast Red chromogen, ACD catalog # 322360). Adjacent tissue section was processed for immunohistochemistry using rabbit polyclonal (Novus Biologicals catalog # NB400-105) at 1.5ug/mL with overnight incubation at 4 degrees Celsius followed by incubation with anti-rabbit IgG VisUCyte HRP Polymer Antibody (Catalog # VC003) and DAB chromogen (yellow-brown). Tissue was counterstained with hematoxylin (blue). Specific staining was localized to glandular cells.
ABCA1 Antibody - BSA Free
NB400-105
Western Blot: TSG101 Antibody (4A10) [NB200-112] - Lane 1: MWM, Lane 2: TSG101 in RAW 264.7 Whole Cell Lysate, Lane 3: Exosome Lysate. Used primary antibody at 1:500 and Donkey Anti-Mouse at 0.5 ug/mL (this could be titrated down to reduce background). Used picogram sensitivity ECL reagents. Image from verified customer review.
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Bioinformatics

Gene Symbol TOM1L2