TOM1 Antibody (PSH03-38)

Western Blot: TOM1 Antibody (PSH03-38) [NBP3-33035] - Western blot analysis of TOM1 on different lysates with Rabbit anti-TOM1 antibody (NBP3-33035) at 1/2,000 dilution. Lane 1: 293T cell lysate (20 ug/Lane) Lane 2: U-2 OS cell lysate (20 ug/Lane) Lane 3: A431 cell lysate (20 ug/Lane) Lane 4: K-562 cell lysate (20 ug/Lane) Lane 5: HepG2 cell lysate (20 ug/Lane) Lane 6: HeLa cell lysate (20 ug/Lane) Lane 7: JAR cell lysate (20 ug/Lane) Lane 8: THP-1 cell lysate (20 ug/Lane) Lane 9: C2C12 cell lysate (20 ug/Lane) Lane 10: PC-12 cell lysate (20 ug/Lane) Lane 11: Mouse heart tissue lysate (40 ug/Lane) Lane 12: Mouse skeletal muscle tissue lysate (40 ug/Lane) Lane 13: Rat liver tissue lysate (40 ug/Lane) Predicted band size: 54 kDa Observed band size: 54 kDa Exposure time: 3 minutes; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-33035) at 1/2,000 dilution was used in 5% NFDM/TBST at 4C overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.

Immunohistochemistry: TOM1 Antibody (PSH03-38) [NBP3-33035] - Immunofluorescence analysis of paraffin-embedded human stomach carcinoma tissue labeling TOM1 with Rabbit anti-TOM1 antibody (NBP3-33035) at 1/200 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 10% negative goat serum for 1 hour at room temperature, washed with PBS, and then probed with the primary antibody (NBP3-33035, green) at 1/200 dilution overnight at 4 ℃, washed with PBS. Goat Anti-Rabbit IgG H&L (iFluor™ 488) was used as the secondary antibody at 1/1,000 dilution. Nuclei were counterstained with DAPI (blue).

Immunohistochemistry: TOM1 Antibody (PSH03-38) [NBP3-33035] - Immunohistochemical analysis of paraffin-embedded human kidney tissue with Rabbit anti-TOM1 antibody (NBP3-33035) at 1/200 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (NBP3-33035) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

• Reactivity: Hu, Mu, Rt
• Applications: WB, IHC
• Clone: PSH03-38
• Clonality: Monoclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Concentration: 1 mg/ml

TOM1 Antibody (PSH03-38) Summary

• Description: Novus Biologicals Rabbit TOM1 Antibody (PSH03-38) (NBP3-33035) is a recombinant monoclonal antibody validated for use in IHC and WB. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Additional Information: Recombinant Monoclonal Antibody
• Immunogen: Recombinant protein within human TOM1 aa 300-450. (Uniprot: O60784)
• Localization: Cytoplasm, Endosome membrane, Early endosome membrane.
• Source: HEK293
• Isotype: IgG
• Clonality: Monoclonal
• Host: Rabbit
• Gene: TOM1
• Purity: Protein A purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin 1:200-1:1000
  • Western Blot 1:2000
• Theoretical MW: 54 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS (pH7.4), 0.1% BSA and 40% Glycerol
• Preservative: 0.05% Sodium Azide
• Concentration: 1 mg/ml
• Purity: Protein A purified

Alternate Names for TOM1 Antibody (PSH03-38)

• ADP-ribosylation factor-binding protein GGA3
• golgi associated, gamma adaptin ear containing, ARF binding protein 3
• golgi-associated, gamma adaptin ear containing, ARF binding protein 3
• KIAA0154Golgi-localized, gamma ear-containing, ARF-binding protein 3

Background

The specific function of this gene has not yet been determined, yet it may involve the translocation of growth factor receptor complexes to the lysosome for degradation. This gene is localized to 22q13.1, with HMOX1 and MCM5 distally and HMG2L1 proximally positioned.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: TOM1