TNPO3 Antibody (JG38-77)

Western Blot: TNPO3 Antibody (JG38-77) [NBP2-75685] - Analysis of TNPO3 on K562 cell (1), HL-60 cell (2) and mouse pancreas tissue (3) lysate using anti-TNPO3 antibody at 1/1,000 dilution.

Immunocytochemistry/Immunofluorescence: TNPO3 Antibody (JG38-77) [NBP2-75685] - Staining TNPO3 in SiHa cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

Immunohistochemistry-Paraffin: TNPO3 Antibody (JG38-77) [NBP2-75685] - Analysis of paraffin-embedded mouse kidney tissue using anti-TNPO3 antibody. Counter stained with hematoxylin.

Immunocytochemistry/Immunofluorescence: TNPO3 Antibody (JG38-77) [NBP2-75685] - Staining TNPO3 in A549 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

Immunocytochemistry/Immunofluorescence: TNPO3 Antibody (JG38-77) [NBP2-75685] - Staining TNPO3 in SH-SY-5Y cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

Immunohistochemistry-Paraffin: TNPO3 Antibody (JG38-77) [NBP2-75685] - Analysis of paraffin-embedded human liver cancer tissue using anti-TNPO3 antibody. Counter stained with hematoxylin.

Immunohistochemistry-Paraffin: TNPO3 Antibody (JG38-77) [NBP2-75685] - Analysis of paraffin-embedded human small intestine tissue using anti-TNPO3 antibody. Counter stained with hematoxylin.

Immunohistochemistry-Paraffin: TNPO3 Antibody (JG38-77) [NBP2-75685] - Analysis of paraffin-embedded mouse lung tissue using anti-TNPO3 antibody. Counter stained with hematoxylin.

Product Discontinued

TNPO3 Antibody (JG38-77) Summary

• Additional Information: Recombinant Monoclonal Antibody.
• Immunogen: Recombinant protein within Human TNPO3 aa 830-923 / 923. (SwissProt: Q9Y5L0 Human; SwissProt: Q6P2B1 Mouse)
• Localization: Nucleus. Cytoplasm.
• Isotype: IgG
• Clonality: Monoclonal
• Host: Rabbit
• Gene: TNPO3
• Purity: Protein A purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence 1:50-1:200
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin 1:50-1:200
  • Western Blot 1:500-1:2000
• Theoretical MW: 104 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: TBS (pH7.4), 0.05% BSA, 40% Glycerol
• Preservative: 0.05% Sodium Azide
• Concentration: 1 mg/ml
• Purity: Protein A purified

Alternate Names for TNPO3 Antibody (JG38-77)

• imp12
• MTR10A
• transportin 3
• transportin-3

Background

TNPO3 is a nuclear import receptor for serine/arginine-rich (SR) proteins, which are essential precursor-mRNA splicing factors (Kataoka et al., 1999 [PubMed 10366588]).[supplied by OMIM]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: TNPO3