TNIP2 Antibody - BSA Free

Western Blot: TNIP2 Antibody [NBP1-32689] - Sample (30 ug of whole cell lysate)A: A431 B: Hela S310% SDS PAGE, antibody diluted at 1:1000.

Immunocytochemistry/Immunofluorescence: TNIP2 Antibody [NBP1-32689] - Paraformaldehyde-fixed HeLa, using antibody at 1:200 dilution.

Immunohistochemistry-Paraffin: TNIP2 Antibody [NBP1-32689] - Paraffin-embedded SAS xenograft, using antibody at 1:500 dilution.

Western Blot: TNIP2 Antibody [NBP1-32689] - A region of PTPN13 containing the PDZ domains is sufficient for association with Flexi cloned Halo-TNIP2. Part of the PTPN13 ORF coding for a 903 aa region, which included the five PDZ domains, was subcloned into FLAG-pcDNA5/FRT and coexpressed in HEK293T cells with or without Halo-TNIP2 (with the valine cloning scar) as indicated. Lysates were subjected to Halo affinity purification and the resulting eluates analysed by SDS-PAGE and Western blotting. Proteins were detected using anti-FLAG(R) M2 mouse monoclonal and anti-TNIP2 rabbit polyclonal primary antibodies, and with IRDye(R) 680LT labeled anti-mouse (red) and IRDye(R) 800CW anti-rabbit (green) secondary antibodies. Proteins were visualized using a LI-COR(R) Odyssey(R) infrared imaging system. Image collected and cropped by CiteAb from the following publication (//www.nature.com/articles/srep08530) licensed under a CC-BY license.

• Reactivity: Hu
• Applications: WB, ICC/IF, IHC
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: BSA Free

TNIP2 Antibody - BSA Free Summary

• Immunogen: Recombinant protein encompassing a sequence within the center region of human TNIP2. The exact sequence is proprietary.
• Localization: Cytoplasm
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: TNIP2
• Purity: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence 1:100-1:1000
  • Immunohistochemistry 1:100-1:1000
  • Immunohistochemistry-Paraffin 1:100-1:1000
  • Western Blot 1:500-1:3000
• Theoretical MW: 49 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Reactivity Notes

Immunogen displays the following percentage of sequence identity for non-tested species: Mouse (82%), Rat (80%).

Packaging, Storage & Formulations

• Storage: Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
• Buffer: 0.1M Tris (pH 7), 0.1M Glycine, 10% Glycerol
• Preservative: 0.01% Thimerosal
• Purity: Antigen Affinity-purified

Alternate Names for TNIP2 Antibody - BSA Free

• A20-binding inhibitor of NF-kappa-B activation 2
• A20-binding inhibitor of NF-kappaB activation-2
• ABIN-2
• ABIN2KLIP
• Fetal liver LKB1-interacting protein
• FLIP1DKFZp434J1313
• MGC4289
• TNFAIP3 interacting protein 2
• TNFAIP3-interacting protein 2

Background

ABIN-2, TNFAIP3 interacting protein 2, binds to the COOH-terminal zinc finger domain of A20. NF-kB activation induced by TNF and interleukin-1 is inhibited by overexpression of ABIN-2. The latter also inhibits NF-kB activation induced by overexpression of receptor-interacting protein or TNF receptor-associated factor 2. A-20 binding Inhibitor of Nuclear Factor-kappaB (NF-kB)Activation (ABIN-2) binds to A-20. ABIN-2 interferes with NF-kB activation upstream of the IKK complex and might contribute to NF-kB inhibitory function of A-20.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

⚠ WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause reproductive toxicity with developmental effects.  For more information go to www.P65Warnings.ca.gov.

Publications for TNIP2 Antibody (NBP1-32689)(2)

Publications using NBP1-32689

• Banks C, Boanca G, Lee Z et al. TNIP2 is a Hub Protein in the NF-B Network with Both Protein and RNA Mediated Interactions. Molecular & Cellular Proteomics 2016-09-08 [PMID: 27609421]
• Banks CA, Boanca G, Lee ZT et al. Proteins interacting with cloning scars: a source of false positive protein-protein interactions Sci Rep 2015-02-23 [PMID: 25704442] (WB, Human)

Bioinformatics

• Gene Symbol: TNIP2
• Entrez:
  • 79155(Human)
• Uniprot:
  • Q8NFZ5(Human)