TMEM70 Overexpression Lysate (Native)

Images

 
Western Blot: TMEM70 Overexpression Lysate (Adult Normal) [NBL1-17101] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for TMEM70.

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB

Order Details

TMEM70 Overexpression Lysate (Native) Summary

Immunogen
The lysate was created in HEK293T cells, using plasmid ID RC201060 and based on accession number NM_017866. The protein contains a C-terminal DDK tag.
Specificity
Homo sapiens transmembrane protein 70 (TMEM70), transcript variant 1, mRNA.
Gene
TMEM70

Applications/Dilutions

Application Notes
This product is intended for use as a positive control in Western Blot. You will receive the lysate (100ug), and an empty vector negative control (100 ug).
Theoretical MW
28.8 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

Storage
Store at -80C. Avoid freeze-thaw cycles.
Buffer
RIPA buffer

Lysate Details for TMEM70

Type
Overexpression
Protein State
Native

Notes

HEK293T cells in 10-cm dishes were transiently transfected with a non-lipid polymer transfection reagent specially designed and manufactured for large volume DNA transfection. Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4, and then centrifuged to clarify the lysate. Protein concentration was measured by BCA protein assay kit.This product is manufactured by and sold under license from OriGene Technologies and its use is limited solely for research purposes.

Alternate Names for TMEM70 Overexpression Lysate (Native)

  • FLJ20533
  • transmembrane protein 70
  • transmembrane protein 70, mitochondrial

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Lysates are guaranteed for 6 months from date of receipt.

Customers Who Viewed This Item Also Viewed...

NBP1-42805
Species: Hu, Mu, Rt, Po, Bv, Xp, Dr(-)
Applications: WB
NBP1-33633
Species: Hu, Rt
Applications: WB, ICC/IF, IHC, IHC-P, IP
NBP1-88888
Species: Hu
Applications: WB, ICC/IF, IHC, IHC-P
NBP1-88885
Species: Hu, Mu, Rt
Applications: WB, ICC/IF, IHC, IHC-P
NBP2-50179
Species: Hu
Applications: WB, Flow, Func, ICC/IF, IP, CyTOF-ready
NBP1-88886
Species: Hu, Mu
Applications: WB, IHC, IHC-P
NBP1-89489
Species: Hu, Mu, Rt
Applications: WB, ICC/IF, IHC, IHC-P
NBP1-33015
Species: Hu, Mu
Applications: WB, ICC/IF, IHC, IHC-P
NB300-543
Species: Hu, Mu, Rt, Am, Bv, Ca, Ft, Fi, Pm, Rb, Sh
Applications: WB, B/N, IHC, IHC-Fr, IHC-P, IP, IF
NB600-335
Species: Hu, Mu, Ec, NA
Applications: WB, ELISA, ICC/IF, IHC-Fr, IP
NB100-2736
Species: Hu, Rt
Applications: WB, IHC, IHC-Fr, IHC-P, IP
NBP2-01860
Species: Hu, Mu, Rt, Ca, Mk
Applications: WB, Flow, ICC/IF, IHC
NBP1-82432
Species: Hu
Applications: WB, ICC/IF, IHC, IHC-P
NBP2-58862
Species: Hu
Applications: ICC/IF
H00011059-M01
Species: Hu
Applications: WB, ELISA, ICC/IF, IHC, IHC-P, IP
NBP1-77274
Species: Hu
Applications: WB, ELISA, ICC/IF, IHC, IHC-P, IF
NBP2-32840
Species: Hu, Mu, Rt, Pm
Applications: WB, Flow, ICC/IF, IHC-P, IP

Publications for TMEM70 Lysate (NBL1-17101) (0)

There are no publications for TMEM70 Lysate (NBL1-17101).
By submitting your publication information earn gift cards and discounts for future purchases.

Reviews for TMEM70 Lysate (NBL1-17101) (0)

There are no reviews for TMEM70 Lysate (NBL1-17101). By submitting a review you will receive an Amazon e-Gift Card or Novus Product Discount.
  • Review with no image -- $10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen
  • Review with an image -- $25/€18/£15/$25 CAD/¥150 Yuan/¥2500 Yen
  • 1st to review with an image -- $50/€35/£30/$50 CAD/¥300 Yuan/¥5000 Yen

Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol

FAQs for TMEM70 Lysate (NBL1-17101) (0)

There are no specific FAQs related to this product. Read our general customer & technical service FAQs.

Additional TMEM70 Products

Bioinformatics Tool for TMEM70 Lysate (NBL1-17101)

Discover related pathways, diseases and genes to TMEM70 Lysate (NBL1-17101). Need help? Read the Bioinformatics Tool Guide for instructions on using this tool.
Visit Tool

Diseases for TMEM70 Lysate (NBL1-17101)

Discover more about diseases related to TMEM70 Lysate (NBL1-17101).
 

Pathways for TMEM70 Lysate (NBL1-17101)

View related products by pathway.

PTMs for TMEM70 Lysate (NBL1-17101)

Learn more about PTMs related to TMEM70 Lysate (NBL1-17101).

Blogs on TMEM70

There are no specific blogs for TMEM70, but you can read our latest blog posts.

Customers Who Bought This Also Bought

c-Myc Antibody
NB600-335
TMEM70 Antibody
NBP1-77131

Contact Information

Product PDFs

Calculators

Concentration Calculator

The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.

=
÷

Review this Product

Be the first to review our TMEM70 Overexpression Lysate (Native) and receive a gift card or discount.

Bioinformatics

Gene Symbol TMEM70