TLK2 Antibody (OTI2D10)

Immunohistochemistry: TLK2 Antibody (2D10) [NBP2-46141] - Analysis of Human endometrium tissue. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120C for 3 min)

Immunohistochemistry: TLK2 Antibody (2D10) [NBP2-46141] - Analysis of Carcinoma of Human kidney tissue. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120C for 3 min)

Immunohistochemistry: TLK2 Antibody (2D10) [NBP2-46141] - Analysis of Human liver tissue. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120C for 3 min)(1:150)

Immunohistochemistry: TLK2 Antibody (2D10) [NBP2-46141] - Analysis of Human lung tissue. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120C for 3 min)(1:150)

Immunohistochemistry: TLK2 Antibody (2D10) [NBP2-46141] - Analysis of Carcinoma of Human lung tissue.(Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120C for 3 min)(1:150)

Immunohistochemistry: TLK2 Antibody (2D10) [NBP2-46141] - Analysis of Adenocarcinoma of Human ovary tissue. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120C for 3 min)

Immunohistochemistry: TLK2 Antibody (2D10) [NBP2-46141] - Analysis of Human pancreas tissue. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120C for 3 min)

Immunohistochemistry: TLK2 Antibody (2D10) [NBP2-46141] - Analysis of Carcinoma of Human pancreas tissue. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120C for 3 min)

Immunohistochemistry: TLK2 Antibody (2D10) [NBP2-46141] - Analysis of Carcinoma of Human thyroid tissue. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120C for 3 min)(1:150)

Immunohistochemistry: TLK2 Antibody (2D10) [NBP2-46141] - Analysis of Adenocarcinoma of Human endometrium tissue. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120C for 3 min)

• Reactivity: Hu, Mu, Rt
• Applications: IHC
• Clone: OTI2D10
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Concentration: 1 mg/ml

TLK2 Antibody (OTI2D10) Summary

• Description: Novus Biologicals Mouse TLK2 Antibody (OTI2D10) (NBP2-46141) is a monoclonal antibody validated for use in IHC. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: Human recombinant protein fragment corresponding to amino acids 1-296 of human TLK2(NP_006843) produced in E.coli.
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Mouse
• Gene: TLK2
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry 1:150
  • Immunohistochemistry-Paraffin
• Theoretical MW: 85.3 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Reactivity Notes

Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Mouse-On-Mouse blocking reagent may be needed for IHC and ICC experiments to reduce high background signal. You can find these reagents under catalog numbers PK-2200-NB and MP-2400-NB. Please contact Technical Support if you have any questions.

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: PBS (pH 7.3), 1.0% BSA and 50% Glycerol
• Preservative: 0.02% Sodium Azide
• Concentration: 1 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for TLK2 Antibody (OTI2D10)

• EC 2.7.11
• EC 2.7.11.1
• MGC44450
• PKU-ALPHA
• serine/threonine-protein kinase tousled-like 2
• tousled-like kinase 2serine/threonine kinase

Background

TLK2 (Tousled-like kinase 2) is a nuclear serine/threonine kinase. It is cell cycle checkpoint and ATM-pathway dependent, with maximal activity in S-phase. It is rapidly and transiently inhibited by phosphorylation following the generation of DNA double-stranded breaks during S-phase. TLK2 is potentially involved in the regulation of chromatin assembly during DNA replication.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: TLK2