TIMP-4 Overexpression Lysate

Images

 
Western Blot: TIMP4 Overexpression Lysate (Adult Normal) [NBL1-16927] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for TIMP4.
Western Blot: TIMP4 Overexpression Lysate (Adult Normal) [NBL1-16927] - Western Blot experiments. Left-Control; Right -Over-expression Lysate for TIMP4

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB

Order Details

TIMP-4 Overexpression Lysate Summary

Description
Transient overexpression lysate of TIMP metallopeptidase inhibitor 4 (TIMP4) The lysate was created in HEK293T cells, using Plasmid ID RC203772 and based on accession number NM_003256. The protein contains a C-MYC/DDK Tag.
Gene
TIMP4

Applications/Dilutions

Dilutions
  • Western Blot
Application Notes
This product is intended for use as a positive control in Western Blot.

You will receive 1 vial of lysate (100ug), 1 vial of empty vector negative control (100ug), and 1 vial of 2xSDS sample buffer (250ul).
Theoretical MW
22.6 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

Storage
Store at -80C. Avoid freeze-thaw cycles.
Buffer
RIPA buffer

Lysate Details for Array

Type
Overexpression

Notes

HEK293T cells in 10-cm dishes were transiently transfected with a non-lipid polymer transfection reagent specially designed and manufactured for large volume DNA transfection. Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4, and then centrifuged to clarify the lysate. Protein concentration was measured by BCA protein assay kit.This product is manufactured by and sold under license from OriGene Technologies and its use is limited solely for research purposes.

Alternate Names for TIMP-4 Overexpression Lysate

  • metalloproteinase inhibitor 4
  • TIMP metallopeptidase inhibitor 4
  • TIMP4
  • TIMP-4
  • tissue inhibitor of metalloproteinase 4
  • Tissue inhibitor of metalloproteinases 4

Background

This gene belongs to the TIMP gene family. The proteins encoded by this gene family are inhibitors of the matrix metalloproteinases, a group of peptidases involved in degradation of the extracellular matrix. The secreted, netrin domain-containing protein encoded by this gene is involved in regulation of platelet aggregation and recruitment and may play role in hormonal regulation and endometrial tissue remodeling. [provided by RefSeq]. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Entrez Gene record to access additional publications.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Lysates are guaranteed for 6 months from date of receipt.

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Publications for TIMP-4 Lysate (NBL1-16927) (0)

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Product General Protocols

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Video Protocols

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Bioinformatics

Gene Symbol TIMP4