TIMP-4 Antibody (SB30c) - Azide and BSA Free

Western Blot: TIMP-4 Antibody (SB30c) [NBP1-28619] - 2 ug Mouse Anti-Human TIMP-4- on pancreatic cancer cell lysate with Goat Anti-Mouse IgG2a, human adsorbed-HRP

Immunocytochemistry/Immunofluorescence: TIMP-4 Antibody (SB30c) [NBP1-28619] - 5 ug/mL Mouse Anti-Human TIMP-4- was stained on cold methanol fixed human pancraetic carcinoma cells with DAPI and Goat Anti-Mouse Ig, human adsorbed-FITC

Immunohistochemistry-Paraffin: TIMP-4 Antibody (SB30c) [NBP1-28619] - Human gastric cancer tissue was stained with Mouse Anti-Human TIMP-4-UNLB (right) followed by followed by HRP conjugated Anti-Mouse Ig secondary antibody, DAB, and hematoxylin.

Product Discontinued

TIMP-4 Antibody (SB30c) - Azide and BSA Free Summary

• Immunogen: Recombinant human TIMP-4 (a.a. 35-224)
• Specificity: Human/Mouse/Rat TIMP-4
• Isotype: IgG2a Kappa
• Clonality: Monoclonal
• Host: Mouse
• Gene: TIMP4
• Purity: Protein A or G purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence
  • Immunohistochemistry 1:10-1:500
  • Immunohistochemistry-Paraffin
  • Western Blot 1:100-1:2000
• Application Notes: Use in IHC-P reported in scientific literature (PMID:31677819)
• Theoretical MW: 25.5 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Reactivity Notes

Mouse reactivity reported in scientific literature (PMID: 31677819).

Packaging, Storage & Formulations

• Storage: Store at 4C. Do not freeze.
• Buffer: 0.1M BBS (pH 8.2)
• Preservative: No Preservative
• Concentration: 0.5 mg/ml
• Purity: Protein A or G purified

Alternate Names for TIMP-4 Antibody (SB30c) - Azide and BSA Free

• metalloproteinase inhibitor 4
• TIMP metallopeptidase inhibitor 4
• TIMP4
• TIMP-4
• tissue inhibitor of metalloproteinase 4
• Tissue inhibitor of metalloproteinases 4

Background

Tissue inhibitors of matrix metalloproteinases (TIMPs) constitute a family of secreted multifunctional proteins which are classified based on structural similarity to each other as well as their ability to inhibit matrix metalloproteinases (MMPs). TIMPs therefore play a critical role in ECM homeostasis but have also been shown to have erythroid-potentiating and cell growth-promoting activities. TIMP-4 (approx. 23kDa), unlike TIMP-1, TIMP-2 and TIMP-3, shows restricted localization to cardiac, neural, breast and muscle tissue. Cardiospecific TIMP-4 seems to play a significant role in regulating the normal cell phenotype since reduced levels elicit cellular transformation. It has been shown to be an inhibitor of MMP-1, MMP-2, MMP-3, MMP-7 and MMP-9 with a preference for MMP-2 and MMP-7. (1-9)

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for TIMP-4 Antibody (NBP1-28619)(1)

Publication using NBP1-28619

• Solga R, Behrens J, Ziemann A et al. CRN2 binds to TIMP4 and MMP14 and promotes perivascular invasion of glioblastoma cells European Journal of Cell Biology 2019-10-01 [PMID: 31677819] (IHC-P, Mouse)

Bioinformatics

• Gene Symbol: TIMP4
• Entrez:
  • 7079(Human)
• Uniprot:
  • Q99727()