TIMP-3 Antibody (183551) [Alexa Fluor® 750]

• Reactivity: Hu
• Applications: WB, IHC, IP, ELISA(Cap), ELISA(Det)
• Clone: 183551
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Alexa Fluor 750

TIMP-3 Antibody (183551) [Alexa Fluor® 750] Summary

• Immunogen: Mouse myeloma cell line NS0-derived recombinant human TIMP-3
  Cys24-Pro211
  Accession # P35625
• Specificity: Detects human TIMP-3 in ELISAs and Western blots. In Western blots, no cross-reactivity with recombinant human TIMP-1, -2, or -4 is observed.
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Mouse
• Purity Statement: Protein A or G purified

Applications/Dilutions

• Dilutions:
  • ELISA Capture (Matched Antibody Pair)
  • ELISA Detection (Matched Antibody Pair)
  • Immunohistochemistry
  • Immunoprecipitation
  • Western Blot

Packaging, Storage & Formulations

• Storage: Protect from light. Do not freeze. 12 months from date of receipt, 2 to 8 °C as supplied
• Buffer: Supplied 0.2mg/ml in 1X PBS with RDF1 and 0.09% Sodium Azide

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for TIMP-3 Antibody (183551) [Alexa Fluor® 750]

• HSMRK222
• K222
• K222TA2
• metalloproteinase inhibitor 3
• MIG-5 protein
• Protein MIG-5
• pseudoinflammatory)
• SFD
• TIMP metallopeptidase inhibitor 3
• TIMP3
• TIMP-3
• tissue inhibitor of metalloproteinase 3 (Sorsby fundus dystrophy
• Tissue inhibitor of metalloproteinases 3

Background

Tissue inhibitors of metalloproteinases (TIMPs) are a family of proteins that regulate the activation and proteolytic activity of the zinc enzymes known as matrix metalloproteinases (MMPs). There are four members of the family, TIMP-1, TIMP-2, TIMP-3 and TIMP-4. TIMP-3 is a glycoprotein with a molecular mass of 30 kDa produced by a wide range of cell types. TIMP-3 inhibits active MMP-mediated proteolysis by forming a non-covalent binary complex with the MMP active site through its N-terminal domain. In addition, TIMP-3 is the only known member of the TIMP family that is an effective inhibitor of ADAMs such as TACE (1).

TIMP-3 is unique among the TIMPs because of its high affinity for binding to the extracellular matrix (2). Point mutations in the TIMP-3 C-terminal domain have been reported to result in Sorsby's fundus dystrophy, a disease leading to macular degeneration and loss of vision.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.